TY - JOUR
T1 - Early hemopoietic progenitors in the peripheral blood of patients with severe aplastic anemia (SAA) after treatment with antilymphocyte globulin (ALG), cyclosporin-A and G-CSF
AU - Bacigalupo, Andrea
AU - Piaggio, Giovanna
AU - Podestà, Marina
AU - Tong, Jie
AU - Pitto, Anna
AU - Figari, Osvaldo
AU - Benvenuto, Federica
AU - Vassallo, Franca
AU - Tedone, Elisabetta
AU - Grassia, Lidia
AU - Van Lint, Maria Teresa
AU - Gualandi, Francesca
AU - Hoffman, Ronald
PY - 1997/3
Y1 - 1997/3
N2 - Background and Objective. We previously reported that patients with acquired severe aplastic anemia (SAA) treated with antilymphocyte globulin (ALG), 6-methylprednisolone, cyclosporin A (CyA) and granulocyte colony- stimulating factor (G-CSF) can mobilize peripheral blood hemopoietic progenitors (PBHP). The aim of the present study was to assess phenotypic and functional properties of these PBHP. Methods. We studied seven patients who underwent 43 leukophereses (median 5) between day +30 and +80 following ALG, while in treatment with CyA and G-CSF. Mobilized peripheral blood hemopoietic progenitors were analyzed using surface markers conventional assays for clonogenic cells (CFU-GM, BFU-E, CFU-GEMM) as well as the recently developed assay for long-term culture initiating cells (LTC-ICs). Results. The proportion of CD34+ cells ranged between 0% and 5.4% median 0.3%) CD34+DR- between 0% and 3.5% (median 0.1%) and CD8+ cells between 3.3% and 56% (median 31%). When light density mononuclear cells (MNC) were plated in vitro, we could grow colony-forming units-granulo-macrophage (CFU-GM) (range 0-45/105 MNC; norma controls 21-200/105 MNC), burst-forming units-erythroid (BFU-E)(range 0-5/105 MNC; normal controls 0-6/105 MNC), multipotent colonies (CFU-GEMM)(range 0-3/105 MNC; normal controls 0-6/105 MNC) and high proliferative potential colony-forming cells (HPP-CFC) (range 03.4/105 MNC). We studied long-term culture-initiating cells (LTC-ICs) in 18 leukophereses from 4 patients; in 7/18 samples LTC-ICs were grown at low frequency (range 0.4-2/106 MNC) (normal controls 5-130/106 MNC), and in one patient in the absence of CFU-GM growth. The total yield of LTC-ICs in two patients was 7.64 and 10.5x102/kg of body weight. Interpretation and Conclusions. This study suggests that cells with the phenotype and in vitro function of early hemopoietic progenitors are found, though in small numbers, in the peripheral blood of patients with SAA after treatment with immunosuppressants and prolonged G-CSF administration. Whether G-CSF- mobilized progenitors contribute to hemopoietic recovery in these patients remains to be determined.
AB - Background and Objective. We previously reported that patients with acquired severe aplastic anemia (SAA) treated with antilymphocyte globulin (ALG), 6-methylprednisolone, cyclosporin A (CyA) and granulocyte colony- stimulating factor (G-CSF) can mobilize peripheral blood hemopoietic progenitors (PBHP). The aim of the present study was to assess phenotypic and functional properties of these PBHP. Methods. We studied seven patients who underwent 43 leukophereses (median 5) between day +30 and +80 following ALG, while in treatment with CyA and G-CSF. Mobilized peripheral blood hemopoietic progenitors were analyzed using surface markers conventional assays for clonogenic cells (CFU-GM, BFU-E, CFU-GEMM) as well as the recently developed assay for long-term culture initiating cells (LTC-ICs). Results. The proportion of CD34+ cells ranged between 0% and 5.4% median 0.3%) CD34+DR- between 0% and 3.5% (median 0.1%) and CD8+ cells between 3.3% and 56% (median 31%). When light density mononuclear cells (MNC) were plated in vitro, we could grow colony-forming units-granulo-macrophage (CFU-GM) (range 0-45/105 MNC; norma controls 21-200/105 MNC), burst-forming units-erythroid (BFU-E)(range 0-5/105 MNC; normal controls 0-6/105 MNC), multipotent colonies (CFU-GEMM)(range 0-3/105 MNC; normal controls 0-6/105 MNC) and high proliferative potential colony-forming cells (HPP-CFC) (range 03.4/105 MNC). We studied long-term culture-initiating cells (LTC-ICs) in 18 leukophereses from 4 patients; in 7/18 samples LTC-ICs were grown at low frequency (range 0.4-2/106 MNC) (normal controls 5-130/106 MNC), and in one patient in the absence of CFU-GM growth. The total yield of LTC-ICs in two patients was 7.64 and 10.5x102/kg of body weight. Interpretation and Conclusions. This study suggests that cells with the phenotype and in vitro function of early hemopoietic progenitors are found, though in small numbers, in the peripheral blood of patients with SAA after treatment with immunosuppressants and prolonged G-CSF administration. Whether G-CSF- mobilized progenitors contribute to hemopoietic recovery in these patients remains to be determined.
KW - Hematopoiesis
KW - Immunosuppressive therapy
KW - Severe aplastic anemia
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=0031468425&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031468425&partnerID=8YFLogxK
M3 - Article
C2 - 9175313
AN - SCOPUS:0031468425
VL - 82
SP - 133
EP - 137
JO - Haematologica
JF - Haematologica
SN - 0390-6078
IS - 2
ER -