Early prediction of treatment outcome in acute myeloid leukemia by measurement of WT1 transcript levels in peripheral blood samples collected after chemotherapy

Daniela Cilloni, Francesca Messa, Francesca Arruga, Ilaria Defilippi, Enrico Gottardi, Milena Fava, Sonia Carturan, Renata Catalano, Enrico Bracco, Emanuela Messa, Paolo Nicoli, Daniela Diverio, Miguel A. Sanz, Giovanni Martinelli, Francesco Lo-Coco, Giuseppe Saglio

Research output: Contribution to journalArticle

Abstract

The Wilms' tumor gene WT1 is a reliable marker for minimal residual disease assessment in acute leukemia patients. The study was designed to demonstrate the potential use of WT1 to establish quality of remission in acute leukemia patients for early identification of patients at high risk of relapse. A prospective study based on a quantitative Real-Time PCR (TaqMan) assay in 562 peripheral blood samples collected from 82 acute leukemia patients at diagnosis and during follow-up was established. The evaluation of WT1 in peripheral blood samples after induction chemotherapy can distinguish the continuous complete remission patients from those who obtain only an "apparent" complete remission and who could relapse within a few months. WT1 helps identify patients at high risk of relapse soon after induction chemotherapy allowing post-induction therapy in high risk patients to be intensified.

Original languageEnglish
Pages (from-to)921-924
Number of pages4
JournalHaematologica
Volume93
Issue number6
DOIs
Publication statusPublished - Jun 2008

Keywords

  • Acute leukemia
  • Minimal residual disease
  • RQ-PCR
  • WT1

ASJC Scopus subject areas

  • Hematology

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    Cilloni, D., Messa, F., Arruga, F., Defilippi, I., Gottardi, E., Fava, M., Carturan, S., Catalano, R., Bracco, E., Messa, E., Nicoli, P., Diverio, D., Sanz, M. A., Martinelli, G., Lo-Coco, F., & Saglio, G. (2008). Early prediction of treatment outcome in acute myeloid leukemia by measurement of WT1 transcript levels in peripheral blood samples collected after chemotherapy. Haematologica, 93(6), 921-924. https://doi.org/10.3324/haematol.12165