Early prophylaxis with recombinant human interleukin-11 prevents spontaneous diabetes in nod mice

Ferdinando Nicoletti; Paola Zaccone; Ignacio Conget; Ramon Gomis; Christer Möller; PierLuigi Meroni; Klaus Bendtzen; William Trepicchio; Stellan Sandler

Early prophylaxis with recombinant human interleukin-11 prevents spontaneous diabetes in nod mice

Ferdinando Nicoletti, Paola Zaccone, Ignacio Conget, Ramon Gomis, Christer Möller, PierLuigi Meroni, Klaus Bendtzen, William Trepicchio, Stellan Sandler

Fondazione Istituto Auxologico Italiano

Research output: Contribution to journal › Article › peer-review

Abstract

We evaluated the effects of recombinant human (rh) interleukin (IL)-11 on the development of spontaneous and cyclophosphamide-induced diabetes in female NOD mice. Prolonged treatment with rhIL-11 10 μg i.p. five consecutive times a week between the 4th and 22nd weeks of age significantly suppressed both development and cumulative incidence of type 1 diabetes. Disease protection was transient because most of the animals developed type 1 diabetes within 3 months of treatment withdrawal. In contrast, rhIL-11 failed to prevent type 1 diabetes when administered for the first time to euglycemic 18-week-old NOD mice. Most likely, this discrepancy was not due to age- dependent differences in the immunological responses of NOD mice to rhIL-11 because staphylococcus aureus enterotoxin B-induced tumor necrosis factor (TNF) and IL-12 production were equally suppressed by rhIL-11 in 12- and 25- week-old NOD mice. Relative to controls, NOD mice pretreated with rhIL-11 also showed significantly diminished blood levels of TNF, interferon-γ, and IL-12 induced by anti-CD3 antibody and/or lipopolysaccharide. The results demonstrate that rhIL-11 has powerful anti-inflammatory effects that are capable of down-regulating early immunodiabetogenic pathways in NOD mice.

Original language	English
Pages (from-to)	2333-2339
Number of pages	7
Journal	Diabetes
Volume	48
Issue number	12
Publication status	Published - Dec 1999

ASJC Scopus subject areas

Internal Medicine
Endocrinology, Diabetes and Metabolism

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Early prophylaxis with recombinant human interleukin-11 prevents spontaneous diabetes in nod mice. / Nicoletti, Ferdinando; Zaccone, Paola; Conget, Ignacio; Gomis, Ramon; Möller, Christer; Meroni, PierLuigi; Bendtzen, Klaus; Trepicchio, William; Sandler, Stellan.

In: Diabetes, Vol. 48, No. 12, 12.1999, p. 2333-2339.

Research output: Contribution to journal › Article › peer-review

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abstract = "We evaluated the effects of recombinant human (rh) interleukin (IL)-11 on the development of spontaneous and cyclophosphamide-induced diabetes in female NOD mice. Prolonged treatment with rhIL-11 10 μg i.p. five consecutive times a week between the 4th and 22nd weeks of age significantly suppressed both development and cumulative incidence of type 1 diabetes. Disease protection was transient because most of the animals developed type 1 diabetes within 3 months of treatment withdrawal. In contrast, rhIL-11 failed to prevent type 1 diabetes when administered for the first time to euglycemic 18-week-old NOD mice. Most likely, this discrepancy was not due to age- dependent differences in the immunological responses of NOD mice to rhIL-11 because staphylococcus aureus enterotoxin B-induced tumor necrosis factor (TNF) and IL-12 production were equally suppressed by rhIL-11 in 12- and 25- week-old NOD mice. Relative to controls, NOD mice pretreated with rhIL-11 also showed significantly diminished blood levels of TNF, interferon-γ, and IL-12 induced by anti-CD3 antibody and/or lipopolysaccharide. The results demonstrate that rhIL-11 has powerful anti-inflammatory effects that are capable of down-regulating early immunodiabetogenic pathways in NOD mice.",

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AU - Nicoletti, Ferdinando

AU - Zaccone, Paola

AU - Conget, Ignacio

AU - Gomis, Ramon

AU - Möller, Christer

AU - Meroni, PierLuigi

AU - Bendtzen, Klaus

AU - Trepicchio, William

AU - Sandler, Stellan

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