Early rise of cytosolic Ca2+ induced by NGF in PC12 and chromaffin cells

Atanasio Pandiella-Alonso, Antonio Malgaroli, Lucia M. Vicentini, Jacopo Meldolesi

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

A rise of cytosolic Ca2+ is induced by NGF in rat pheochromocytoma PC12 and bovine chromaffin cells investigated (both in suspension and while attached to polyornithine-coated glass slides) by fluorescence techniques (with quin-2 and fura-2). The effect of NGF on [Ca2+]i is delayed (30-40 s of lag phase), slow (tf1/2 = 40 s), relatively small (+50-75%) and persistent (over 10 min). It is due to Ca2+ influx (requires extra-cellular Ca2+ > 10 μM) through a pathway different from the voltage-gated Ca2+ channel, possibly accompanied by intracellular Ca2+ redistribution, and might play a messenger role in NGF action.

Original languageEnglish
Pages (from-to)48-51
Number of pages4
JournalFEBS Letters
Volume208
Issue number1
DOIs
Publication statusPublished - Nov 10 1986

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Chromaffin Cells
PC12 Cells
Nerve Growth Factor
Fura-2
Pheochromocytoma
Glass
Rats
Suspensions
Fluorescence
Electric potential

Keywords

  • Nerve growth factor Ca Second messenger Phosphatidylinositol Quin-2 Fura-2

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Early rise of cytosolic Ca2+ induced by NGF in PC12 and chromaffin cells. / Pandiella-Alonso, Atanasio; Malgaroli, Antonio; Vicentini, Lucia M.; Meldolesi, Jacopo.

In: FEBS Letters, Vol. 208, No. 1, 10.11.1986, p. 48-51.

Research output: Contribution to journalArticle

Pandiella-Alonso, Atanasio ; Malgaroli, Antonio ; Vicentini, Lucia M. ; Meldolesi, Jacopo. / Early rise of cytosolic Ca2+ induced by NGF in PC12 and chromaffin cells. In: FEBS Letters. 1986 ; Vol. 208, No. 1. pp. 48-51.
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