Abstract
We investigated the effects of oxygen (O2)/glucose deprivation on intracellular sodium concentration ([Na+](i)) of cortical pyramidal cells in a slice preparation of rat frontal cortex. Intracellular recordings were combined with microfluorometric measurements of [Na+](i) using the Na+-sensitive dye sodium-binding benzofuran isophthalate (SBFI). Deprivation of O2/glucose caused an initial membrane hyperpolarization that was followed by a slowly developing large depolarization. Levels of [Na+](i) started to increase significantly during the phase of membrane hyperpolarization. Neither tetrodotoxin, a combination of ionotropic and metabotropic glutamate receptor antagonists (D-amino-phosphonovalerate, 6-cyano-7-nitroquinoxaline-2,3-dione plus S-methyl-4-carboxyphenylglycine) nor bepridil, an inhibitor of the Na+/Ca2+-exchanger, affected these responses to O2/ glucose. The present results demonstrate that, in cortical neurons, O2/glucose deprivation induces an early rise in [Na+](i) which cannot be ascribed to the activity of voltage gated Na+-channels, glutamate receptors or of the Na+/Ca2+-exchanger.
| Original language | English |
|---|---|
| Pages (from-to) | 3572-3574 |
| Number of pages | 3 |
| Journal | European Journal of Neuroscience |
| Volume | 10 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 1998 |
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Keywords
- Electrophysiology
- Glutamate receptors
- Ischaemia
- Na/Ca-exchanger
- Optical recordings
- Sodium channels
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Early sodium elevations induced by combined oxygen and glucose deprivation in pyramidal cortical neurons. / Pisani, A.; Calabresi, P.; Tozzi, A.; Bernardi, G.; Knopfel, T.
In: European Journal of Neuroscience, Vol. 10, No. 11, 1998, p. 3572-3574.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Early sodium elevations induced by combined oxygen and glucose deprivation in pyramidal cortical neurons
AU - Pisani, A.
AU - Calabresi, P.
AU - Tozzi, A.
AU - Bernardi, G.
AU - Knopfel, T.
PY - 1998
Y1 - 1998
N2 - We investigated the effects of oxygen (O2)/glucose deprivation on intracellular sodium concentration ([Na+](i)) of cortical pyramidal cells in a slice preparation of rat frontal cortex. Intracellular recordings were combined with microfluorometric measurements of [Na+](i) using the Na+-sensitive dye sodium-binding benzofuran isophthalate (SBFI). Deprivation of O2/glucose caused an initial membrane hyperpolarization that was followed by a slowly developing large depolarization. Levels of [Na+](i) started to increase significantly during the phase of membrane hyperpolarization. Neither tetrodotoxin, a combination of ionotropic and metabotropic glutamate receptor antagonists (D-amino-phosphonovalerate, 6-cyano-7-nitroquinoxaline-2,3-dione plus S-methyl-4-carboxyphenylglycine) nor bepridil, an inhibitor of the Na+/Ca2+-exchanger, affected these responses to O2/ glucose. The present results demonstrate that, in cortical neurons, O2/glucose deprivation induces an early rise in [Na+](i) which cannot be ascribed to the activity of voltage gated Na+-channels, glutamate receptors or of the Na+/Ca2+-exchanger.
AB - We investigated the effects of oxygen (O2)/glucose deprivation on intracellular sodium concentration ([Na+](i)) of cortical pyramidal cells in a slice preparation of rat frontal cortex. Intracellular recordings were combined with microfluorometric measurements of [Na+](i) using the Na+-sensitive dye sodium-binding benzofuran isophthalate (SBFI). Deprivation of O2/glucose caused an initial membrane hyperpolarization that was followed by a slowly developing large depolarization. Levels of [Na+](i) started to increase significantly during the phase of membrane hyperpolarization. Neither tetrodotoxin, a combination of ionotropic and metabotropic glutamate receptor antagonists (D-amino-phosphonovalerate, 6-cyano-7-nitroquinoxaline-2,3-dione plus S-methyl-4-carboxyphenylglycine) nor bepridil, an inhibitor of the Na+/Ca2+-exchanger, affected these responses to O2/ glucose. The present results demonstrate that, in cortical neurons, O2/glucose deprivation induces an early rise in [Na+](i) which cannot be ascribed to the activity of voltage gated Na+-channels, glutamate receptors or of the Na+/Ca2+-exchanger.
KW - Electrophysiology
KW - Glutamate receptors
KW - Ischaemia
KW - Na/Ca-exchanger
KW - Optical recordings
KW - Sodium channels
UR - http://www.scopus.com/inward/record.url?scp=0032466095&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032466095&partnerID=8YFLogxK
U2 - 10.1046/j.1460-9568.1998.00398.x
DO - 10.1046/j.1460-9568.1998.00398.x
M3 - Article
C2 - 9824470
AN - SCOPUS:0032466095
VL - 10
SP - 3572
EP - 3574
JO - European Journal of Neuroscience
JF - European Journal of Neuroscience
SN - 0953-816X
IS - 11
ER -