Earthworm leukocytes that are not phagocytic and cross-react with several human epitopes can kill human tumor cell lines

Andrea Cossarizza, Edwin L. Cooper, Michael M. Suzuki, Stefano Salvioli, Miriam Capri, Giorgia Gri, Daniela Quaglino, Claudio Franceschi

Research output: Contribution to journalArticlepeer-review

Abstract

Earthworm coelomocytes (leukocytes) effect cytotoxicity at significantly high levels against the NK-sensitive, human tumor cell line, K562, and the NK-resistant targets (U937, BSM, CEM). By cytofluorimetric analyses using mouse anti-human monoclonal antibodies and by morphological evaluations, two types of coelomocytes were identified: (1) small (8-11 μm) electron-dense cells (SC): CD11a+, CD45RA+, CD45RO+, CDw49b+, CD54+, β2-m+, and Thy-1+; (2) large (1215 μm) electron-lucent cells (LC) that are negative for these markers. Both cell types were negative for other CD and MHC class I and class II markers. SC were active during recognition, rapidly binding to targets; LC were phagocytic. Release of 51Cr revealed rapid, significant, and equal levels of killing at 4°, 20°, and 37°C. We propose that primitive NK-like activity appeared early in evolution.

Original languageEnglish
Pages (from-to)174-182
Number of pages9
JournalExperimental Cell Research
Volume224
Issue number1
DOIs
Publication statusPublished - Apr 10 1996

ASJC Scopus subject areas

  • Cell Biology

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