TY - JOUR
T1 - Earthworm leukocytes that are not phagocytic and cross-react with several human epitopes can kill human tumor cell lines
AU - Cossarizza, Andrea
AU - Cooper, Edwin L.
AU - Suzuki, Michael M.
AU - Salvioli, Stefano
AU - Capri, Miriam
AU - Gri, Giorgia
AU - Quaglino, Daniela
AU - Franceschi, Claudio
PY - 1996/4/10
Y1 - 1996/4/10
N2 - Earthworm coelomocytes (leukocytes) effect cytotoxicity at significantly high levels against the NK-sensitive, human tumor cell line, K562, and the NK-resistant targets (U937, BSM, CEM). By cytofluorimetric analyses using mouse anti-human monoclonal antibodies and by morphological evaluations, two types of coelomocytes were identified: (1) small (8-11 μm) electron-dense cells (SC): CD11a+, CD45RA+, CD45RO+, CDw49b+, CD54+, β2-m+, and Thy-1+; (2) large (1215 μm) electron-lucent cells (LC) that are negative for these markers. Both cell types were negative for other CD and MHC class I and class II markers. SC were active during recognition, rapidly binding to targets; LC were phagocytic. Release of 51Cr revealed rapid, significant, and equal levels of killing at 4°, 20°, and 37°C. We propose that primitive NK-like activity appeared early in evolution.
AB - Earthworm coelomocytes (leukocytes) effect cytotoxicity at significantly high levels against the NK-sensitive, human tumor cell line, K562, and the NK-resistant targets (U937, BSM, CEM). By cytofluorimetric analyses using mouse anti-human monoclonal antibodies and by morphological evaluations, two types of coelomocytes were identified: (1) small (8-11 μm) electron-dense cells (SC): CD11a+, CD45RA+, CD45RO+, CDw49b+, CD54+, β2-m+, and Thy-1+; (2) large (1215 μm) electron-lucent cells (LC) that are negative for these markers. Both cell types were negative for other CD and MHC class I and class II markers. SC were active during recognition, rapidly binding to targets; LC were phagocytic. Release of 51Cr revealed rapid, significant, and equal levels of killing at 4°, 20°, and 37°C. We propose that primitive NK-like activity appeared early in evolution.
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U2 - 10.1006/excr.1996.0125
DO - 10.1006/excr.1996.0125
M3 - Article
C2 - 8612683
AN - SCOPUS:0029997392
VL - 224
SP - 174
EP - 182
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 1
ER -