EBP1 is a nucleolar growth-regulating protein that is part of pre-ribosomal ribonucleoprotein complexes

Massimo Squatrito, Monica Mancino, Maddalena Donzelli, Liliana B. Areces, Giulio F. Draetta

Research output: Contribution to journalArticle

Abstract

EBP1 was identified as a protein that interacts with the ErbB-3 receptor and possibly contributes to transducing growth regulatory signals. The existence of EBP1 homologs across species from simple eukaryotes to humans and its wide tissue expression pattern suggest that EBP1 acts as a general signaling molecule. We provide evidence that EBP1 is localized to the cytoplasm and to the nucleolus, and that its nucleolar localization requires amino-acid sequences present at both the amino-and carboxy-terminus of the molecule. We also show that EBP1 overexpression inhibits proliferation of human fibroblasts, and that this effect is linked to its nucleolar localization. Using mass spectrometry we demonstrate that EBP1 is part of ribonucleoprotein complexes and associates with different rRNA species. It is becoming clear that cell growth and proliferation are actively coordinated with rRNA processing and ribosome assembly. Our findings indicate that EBP1 is a nucleolar growth-regulating protein, and we propose that it could represent a new link between ribosome biosynthesis and cell proliferation.

Original languageEnglish
Pages (from-to)4454-4465
Number of pages12
JournalOncogene
Volume23
Issue number25
DOIs
Publication statusPublished - May 27 2004

Fingerprint

Ribonucleoproteins
Ribosomes
ErbB-3 Receptor
Growth
Cell Proliferation
Proteins
Eukaryota
Amino Acid Sequence
Mass Spectrometry
Cytoplasm
Fibroblasts

Keywords

  • Growth control
  • Localization signal
  • Nucleolus
  • Proteohics
  • Ribosome assembly
  • rRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

EBP1 is a nucleolar growth-regulating protein that is part of pre-ribosomal ribonucleoprotein complexes. / Squatrito, Massimo; Mancino, Monica; Donzelli, Maddalena; Areces, Liliana B.; Draetta, Giulio F.

In: Oncogene, Vol. 23, No. 25, 27.05.2004, p. 4454-4465.

Research output: Contribution to journalArticle

Squatrito, Massimo ; Mancino, Monica ; Donzelli, Maddalena ; Areces, Liliana B. ; Draetta, Giulio F. / EBP1 is a nucleolar growth-regulating protein that is part of pre-ribosomal ribonucleoprotein complexes. In: Oncogene. 2004 ; Vol. 23, No. 25. pp. 4454-4465.
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