Echinococcus multilocularis and Echinococcus shiquicus in a small mammal community on the eastern Tibetan Plateau: Host species composition, molecular prevalence, and epidemiological implications

Xu Wang, Jiayu Liu, Qingqiu Zuo, Zhiqiang Mu, Xiaodong Weng, Xiaohui Sun, Junyao Wang, Belgees Boufana, Philip S. Craig, Patrick Giraudoux, Francis Raoul, Zhenghuan Wang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: The eastern part of the Tibetan Plateau is now recognized as an endemic region with the highest reported human infection rates in the world of human alveolar echinococcosis (AE) caused by Echinococcus multilocularis. Existing epidemiological studies on AE have mainly focused on the synanthropic environment, while basic parasitological and ecological aspects in wildlife host species remain largely unknown, especially for small mammal hosts. Therefore, we examined small mammal host species composition, occurrence, and the prevalence of both E. multilocularis and E. shiquicus in Shiqu County (Sichuan Province, China), eastern Tibetan Plateau. Results: In total, 346 small mammals from five rodent and one pika species were trapped from four randomly set 0.25 ha square plots. Two vole species, Lasiopodomys fuscus (n = 144) and Microtus limnophilus (n = 44), and the plateau pika (Ochotona curzoniae) (n = 135), were the three most-dominant species trapped. Although protoscoleces of E. multilocularis and E. shiquicus were only observed in L. fuscus and O. curzoniae, respectively, cox1 and nad1 gene DNA of E. shiquicus was detected in all the small mammal species except for Neodon irene, whereas E. multilocularis was detected in the three most-dominant species. The overall molecular prevalence of Echinococcus species was 5.8 (95% CI: 3.3-8.2%) ~ 10.7% (95% CI: 7.4-14.0%) (the conservative prevalence to the maximum prevalence with 95% CI in parentheses), whereas for E. multilocularis it was 4.3 (95% CI: 2.2-6.5%) ~ 6.7% (95% CI: 4.0-9.3%), and 1.5 (95% CI: 0.2-2.7%) ~ 4.1% (95% CI: 2.0-6.1%) for E. shiquicus. The prevalence of both E. multilocularis and E. shiquicus, was significantly higher in rodents (mainly voles) than in pikas. Phylogenetic analyses revealed that Echinococcus haplotypes of cox1 from small mammal hosts were actively involved in the sylvatic and anthropogenic transmission cycles of E. multilocularis in the eastern Tibetan Plateau. Conclusions: In contrast to previous studies, the current results indicated that rodent species, rather than pikas, are probably more important natural intermediate hosts of E. multilocularis and E. shiquicus in the eastern Tibetan Plateau. Thus, understanding interspecific dynamics between rodents and pikas is essential to studies of the echinococcosis transmission mechanism and human echinococcosis prevention in local communities.

Original languageEnglish
Article number302
JournalParasites and Vectors
Volume11
Issue number1
DOIs
Publication statusPublished - May 16 2018
Externally publishedYes

Fingerprint

Echinococcus multilocularis
Echinococcus
Lagomorpha
Mammals
Arvicolinae
Rodentia
Echinococcosis
Haplotypes
Epidemiologic Studies
China

Keywords

  • E. shiquicus
  • Echinococcus multilocularis
  • Prevalence
  • Small mammal
  • Tibetan Plateau

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Echinococcus multilocularis and Echinococcus shiquicus in a small mammal community on the eastern Tibetan Plateau : Host species composition, molecular prevalence, and epidemiological implications. / Wang, Xu; Liu, Jiayu; Zuo, Qingqiu; Mu, Zhiqiang; Weng, Xiaodong; Sun, Xiaohui; Wang, Junyao; Boufana, Belgees; Craig, Philip S.; Giraudoux, Patrick; Raoul, Francis; Wang, Zhenghuan.

In: Parasites and Vectors, Vol. 11, No. 1, 302, 16.05.2018.

Research output: Contribution to journalArticle

Wang, Xu ; Liu, Jiayu ; Zuo, Qingqiu ; Mu, Zhiqiang ; Weng, Xiaodong ; Sun, Xiaohui ; Wang, Junyao ; Boufana, Belgees ; Craig, Philip S. ; Giraudoux, Patrick ; Raoul, Francis ; Wang, Zhenghuan. / Echinococcus multilocularis and Echinococcus shiquicus in a small mammal community on the eastern Tibetan Plateau : Host species composition, molecular prevalence, and epidemiological implications. In: Parasites and Vectors. 2018 ; Vol. 11, No. 1.
@article{a794f93d4bca48d8a7dfb55a53a5eae2,
title = "Echinococcus multilocularis and Echinococcus shiquicus in a small mammal community on the eastern Tibetan Plateau: Host species composition, molecular prevalence, and epidemiological implications",
abstract = "Background: The eastern part of the Tibetan Plateau is now recognized as an endemic region with the highest reported human infection rates in the world of human alveolar echinococcosis (AE) caused by Echinococcus multilocularis. Existing epidemiological studies on AE have mainly focused on the synanthropic environment, while basic parasitological and ecological aspects in wildlife host species remain largely unknown, especially for small mammal hosts. Therefore, we examined small mammal host species composition, occurrence, and the prevalence of both E. multilocularis and E. shiquicus in Shiqu County (Sichuan Province, China), eastern Tibetan Plateau. Results: In total, 346 small mammals from five rodent and one pika species were trapped from four randomly set 0.25 ha square plots. Two vole species, Lasiopodomys fuscus (n = 144) and Microtus limnophilus (n = 44), and the plateau pika (Ochotona curzoniae) (n = 135), were the three most-dominant species trapped. Although protoscoleces of E. multilocularis and E. shiquicus were only observed in L. fuscus and O. curzoniae, respectively, cox1 and nad1 gene DNA of E. shiquicus was detected in all the small mammal species except for Neodon irene, whereas E. multilocularis was detected in the three most-dominant species. The overall molecular prevalence of Echinococcus species was 5.8 (95{\%} CI: 3.3-8.2{\%}) ~ 10.7{\%} (95{\%} CI: 7.4-14.0{\%}) (the conservative prevalence to the maximum prevalence with 95{\%} CI in parentheses), whereas for E. multilocularis it was 4.3 (95{\%} CI: 2.2-6.5{\%}) ~ 6.7{\%} (95{\%} CI: 4.0-9.3{\%}), and 1.5 (95{\%} CI: 0.2-2.7{\%}) ~ 4.1{\%} (95{\%} CI: 2.0-6.1{\%}) for E. shiquicus. The prevalence of both E. multilocularis and E. shiquicus, was significantly higher in rodents (mainly voles) than in pikas. Phylogenetic analyses revealed that Echinococcus haplotypes of cox1 from small mammal hosts were actively involved in the sylvatic and anthropogenic transmission cycles of E. multilocularis in the eastern Tibetan Plateau. Conclusions: In contrast to previous studies, the current results indicated that rodent species, rather than pikas, are probably more important natural intermediate hosts of E. multilocularis and E. shiquicus in the eastern Tibetan Plateau. Thus, understanding interspecific dynamics between rodents and pikas is essential to studies of the echinococcosis transmission mechanism and human echinococcosis prevention in local communities.",
keywords = "E. shiquicus, Echinococcus multilocularis, Prevalence, Small mammal, Tibetan Plateau",
author = "Xu Wang and Jiayu Liu and Qingqiu Zuo and Zhiqiang Mu and Xiaodong Weng and Xiaohui Sun and Junyao Wang and Belgees Boufana and Craig, {Philip S.} and Patrick Giraudoux and Francis Raoul and Zhenghuan Wang",
year = "2018",
month = "5",
day = "16",
doi = "10.1186/s13071-018-2873-x",
language = "English",
volume = "11",
journal = "Parasites and Vectors",
issn = "1756-3305",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Echinococcus multilocularis and Echinococcus shiquicus in a small mammal community on the eastern Tibetan Plateau

T2 - Host species composition, molecular prevalence, and epidemiological implications

AU - Wang, Xu

AU - Liu, Jiayu

AU - Zuo, Qingqiu

AU - Mu, Zhiqiang

AU - Weng, Xiaodong

AU - Sun, Xiaohui

AU - Wang, Junyao

AU - Boufana, Belgees

AU - Craig, Philip S.

AU - Giraudoux, Patrick

AU - Raoul, Francis

AU - Wang, Zhenghuan

PY - 2018/5/16

Y1 - 2018/5/16

N2 - Background: The eastern part of the Tibetan Plateau is now recognized as an endemic region with the highest reported human infection rates in the world of human alveolar echinococcosis (AE) caused by Echinococcus multilocularis. Existing epidemiological studies on AE have mainly focused on the synanthropic environment, while basic parasitological and ecological aspects in wildlife host species remain largely unknown, especially for small mammal hosts. Therefore, we examined small mammal host species composition, occurrence, and the prevalence of both E. multilocularis and E. shiquicus in Shiqu County (Sichuan Province, China), eastern Tibetan Plateau. Results: In total, 346 small mammals from five rodent and one pika species were trapped from four randomly set 0.25 ha square plots. Two vole species, Lasiopodomys fuscus (n = 144) and Microtus limnophilus (n = 44), and the plateau pika (Ochotona curzoniae) (n = 135), were the three most-dominant species trapped. Although protoscoleces of E. multilocularis and E. shiquicus were only observed in L. fuscus and O. curzoniae, respectively, cox1 and nad1 gene DNA of E. shiquicus was detected in all the small mammal species except for Neodon irene, whereas E. multilocularis was detected in the three most-dominant species. The overall molecular prevalence of Echinococcus species was 5.8 (95% CI: 3.3-8.2%) ~ 10.7% (95% CI: 7.4-14.0%) (the conservative prevalence to the maximum prevalence with 95% CI in parentheses), whereas for E. multilocularis it was 4.3 (95% CI: 2.2-6.5%) ~ 6.7% (95% CI: 4.0-9.3%), and 1.5 (95% CI: 0.2-2.7%) ~ 4.1% (95% CI: 2.0-6.1%) for E. shiquicus. The prevalence of both E. multilocularis and E. shiquicus, was significantly higher in rodents (mainly voles) than in pikas. Phylogenetic analyses revealed that Echinococcus haplotypes of cox1 from small mammal hosts were actively involved in the sylvatic and anthropogenic transmission cycles of E. multilocularis in the eastern Tibetan Plateau. Conclusions: In contrast to previous studies, the current results indicated that rodent species, rather than pikas, are probably more important natural intermediate hosts of E. multilocularis and E. shiquicus in the eastern Tibetan Plateau. Thus, understanding interspecific dynamics between rodents and pikas is essential to studies of the echinococcosis transmission mechanism and human echinococcosis prevention in local communities.

AB - Background: The eastern part of the Tibetan Plateau is now recognized as an endemic region with the highest reported human infection rates in the world of human alveolar echinococcosis (AE) caused by Echinococcus multilocularis. Existing epidemiological studies on AE have mainly focused on the synanthropic environment, while basic parasitological and ecological aspects in wildlife host species remain largely unknown, especially for small mammal hosts. Therefore, we examined small mammal host species composition, occurrence, and the prevalence of both E. multilocularis and E. shiquicus in Shiqu County (Sichuan Province, China), eastern Tibetan Plateau. Results: In total, 346 small mammals from five rodent and one pika species were trapped from four randomly set 0.25 ha square plots. Two vole species, Lasiopodomys fuscus (n = 144) and Microtus limnophilus (n = 44), and the plateau pika (Ochotona curzoniae) (n = 135), were the three most-dominant species trapped. Although protoscoleces of E. multilocularis and E. shiquicus were only observed in L. fuscus and O. curzoniae, respectively, cox1 and nad1 gene DNA of E. shiquicus was detected in all the small mammal species except for Neodon irene, whereas E. multilocularis was detected in the three most-dominant species. The overall molecular prevalence of Echinococcus species was 5.8 (95% CI: 3.3-8.2%) ~ 10.7% (95% CI: 7.4-14.0%) (the conservative prevalence to the maximum prevalence with 95% CI in parentheses), whereas for E. multilocularis it was 4.3 (95% CI: 2.2-6.5%) ~ 6.7% (95% CI: 4.0-9.3%), and 1.5 (95% CI: 0.2-2.7%) ~ 4.1% (95% CI: 2.0-6.1%) for E. shiquicus. The prevalence of both E. multilocularis and E. shiquicus, was significantly higher in rodents (mainly voles) than in pikas. Phylogenetic analyses revealed that Echinococcus haplotypes of cox1 from small mammal hosts were actively involved in the sylvatic and anthropogenic transmission cycles of E. multilocularis in the eastern Tibetan Plateau. Conclusions: In contrast to previous studies, the current results indicated that rodent species, rather than pikas, are probably more important natural intermediate hosts of E. multilocularis and E. shiquicus in the eastern Tibetan Plateau. Thus, understanding interspecific dynamics between rodents and pikas is essential to studies of the echinococcosis transmission mechanism and human echinococcosis prevention in local communities.

KW - E. shiquicus

KW - Echinococcus multilocularis

KW - Prevalence

KW - Small mammal

KW - Tibetan Plateau

UR - http://www.scopus.com/inward/record.url?scp=85047212148&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047212148&partnerID=8YFLogxK

U2 - 10.1186/s13071-018-2873-x

DO - 10.1186/s13071-018-2873-x

M3 - Article

C2 - 29769131

AN - SCOPUS:85047212148

VL - 11

JO - Parasites and Vectors

JF - Parasites and Vectors

SN - 1756-3305

IS - 1

M1 - 302

ER -