Eczema and food allergy in an Italian pediatric cohort: No association with TLR-2 and TLR-4 polymorphisms

Ellena Galli, A. Ciucci, S. Cersosimo, C. Pagnini, S. Avitabile, G. Mancino, G. Delle Fave, V. D. Corleto

Research output: Contribution to journalArticlepeer-review

Abstract

Recent studies have indicated that Toll-like receptor polymorphisms or their impaired signalling, specifically TLR-2 and TLR-4, were correlated with a higher risk for allergy. The purpose of this study is to evaluate the associations of TRL-2 and TRL-4 single nucleotide polymorphisms (SNP) and atopic traits in a cohort of 159 Italian allergic children (102 affected by eczema and 57 by IgE-mediated food allergy) and 147 healthy controls recruited in Rome, Italy. DNA was isolated from the peripheral blood and TLR-2 R753Q/TLR-4 D299G polymorphisms were determined by TaqMan MGB probes using Real-Time PCR technique. In the control group, the TLR-2 polymorphism R753Q had a prevalence of 2.5% while the frequency of the TLR-4 D299G was 12%. None of the 159 allergic patients showed the R753Q SNP. By contrast, 7/57 patients with food allergy (12%) and 6/102 subjects with eczema (6%) carried the TLR-4 mutation. In our cohort, no evidence of correlation between TLR-2 or TLR-4 polymorphism and eczema and food allergy incidence and/or severity was found. Further studies are needed to clarify the possible role of TLR-2 and TLR-4 polymorphism in allergic disease, in Italian children.

Original languageEnglish
Pages (from-to)671-675
Number of pages5
JournalInternational Journal of Immunopathology and Pharmacology
Volume23
Issue number2
Publication statusPublished - Apr 2010

Keywords

  • Eczema
  • Food allergy
  • Polymorphisms
  • TLR-2
  • TLR-4

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

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