EDG3 and SHC3 on chromosome 9q22 are co-amplified in human ependymomas

Lorenzo Magrassi, Nicola Marziliano, Frediano Inzani, Pamela Cassini, Ilaria Chiaranda, Miran Skrap, Stefano Pizzolito, Cesare Arienta, Eloisa Arbustini

Research output: Contribution to journalArticle

Abstract

By qPCR we found that EDG3 and SHC3 were amplified in 60% of ependymomas but none in choroid plexus papillomas. In ependymomas EDG3 and SHC3 amplification increased Shc3 protein levels while EDG3 was less affected. Both proteins were co-immunoprecipitated from ependymoma and Shc3 was tyrosine phosphorylated thus presumably active. We showed by digestion with N-glycosidase-F that EDG3 was glycosylated indicating that EDG3 protein was not retained in the endoplasmic reticulum. The co-immunoprecipitation of Shc3 and EDG3 proteins from ependymomas with amplification of SHC3 and EDG3 genes suggests that the two proteins co-operate and are important for ependymomas in vivo.

Original languageEnglish
Pages (from-to)36-42
Number of pages7
JournalCancer Letters
Volume290
Issue number1
DOIs
Publication statusPublished - Apr 1 2010

Keywords

  • Choroid plexus papilloma
  • EDG3
  • Ependymoma
  • Gene amplification
  • SHC3

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Magrassi, L., Marziliano, N., Inzani, F., Cassini, P., Chiaranda, I., Skrap, M., Pizzolito, S., Arienta, C., & Arbustini, E. (2010). EDG3 and SHC3 on chromosome 9q22 are co-amplified in human ependymomas. Cancer Letters, 290(1), 36-42. https://doi.org/10.1016/j.canlet.2009.08.023