eEF1A phosphorylation in the nucleus of insulin-stimulated C2C12 myoblasts

Ser53 is a novel substrate for protein kinase C BI

Manuela Piazzi, Alberto Bavelloni, Irene Faenza, William Blalock, Andrea Urbani, Simona D'Aguanno, Roberta Fiume, Giulia Ramazzotti, Nadir Mario Maraldi, Lucio Cocco

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Recent data indicate that some PKC isoforms are translocated to the nucleus, in response to certain stimuli, where they play an important role in nuclear signaling events. To identify novel interacting proteins of conventional PKC (cPKC) at the nuclear level during myogenesis and to find new PKC isozyme-specific phosphosubstrates, we performed a proteomics analysis of immuno-precipitated nuclear samples from mouse myoblast C2C12 cells following insulin administration. Using a phospho(Ser)-PKC substrate antibody, specific interacting proteins were identified by LC-MS/MS spectrometry. A total of 16 proteins with the exact and complete motif recognized by the phospho-cPKC substrate antibody were identified; among these, particular interest was given to eukaryotic elongation factor 1α (eEF1A). Nuclear eEF1A was focalized in the nucleoli, and its expression was observed to increase following insulin treatment. Of the cPKC isoforms, only PKCβI was demonstrated to be expressed in the nucleus of C2C12 myocytes and to co-immunoprecipitate with eEF1A. In-depth analysis using site-directed mutagenesis revealed that PKCβI could phosphorylate Ser53 of the eEF1A2 isoform and that the association between eEF1A2 and PKCβI was dependent on the phosphorylation status of eEF1A2.

Original languageEnglish
Pages (from-to)2719-2728
Number of pages10
JournalMolecular and Cellular Proteomics
Volume9
Issue number12
DOIs
Publication statusPublished - Dec 2010

Fingerprint

Phosphorylation
Myoblasts
Protein Kinase C
Protein Isoforms
Insulin
Substrates
Peptide Elongation Factor 1
Mutagenesis
Proteins
Muscle Development
Antibodies
Site-Directed Mutagenesis
Proteomics
Muscle Cells
Spectrometry
Isoenzymes
Spectrum Analysis
Association reactions

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Cite this

eEF1A phosphorylation in the nucleus of insulin-stimulated C2C12 myoblasts : Ser53 is a novel substrate for protein kinase C BI. / Piazzi, Manuela; Bavelloni, Alberto; Faenza, Irene; Blalock, William; Urbani, Andrea; D'Aguanno, Simona; Fiume, Roberta; Ramazzotti, Giulia; Maraldi, Nadir Mario; Cocco, Lucio.

In: Molecular and Cellular Proteomics, Vol. 9, No. 12, 12.2010, p. 2719-2728.

Research output: Contribution to journalArticle

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