EEF1Bγ binds the Che-1 and TP53 gene promoters and their transcripts

Cinzia Pisani, Annalisa Onori, Francesca Gabanella, Francesca Delle Monache, Antonella Borreca, Martine Ammassari-Teule, Maurizio Fanciulli, Maria Grazia Di Certo, Claudio Passananti, Nicoletta Corbi

Research output: Contribution to journalArticle

Abstract

Background: We have previously shown that the eukaryotic elongation factor subunit 1B gamma (eEF1Bγ) interacts with the RNA polymerase II (pol II) alpha-like subunit "C" (POLR2C), alone or complexed, in the pol II enzyme. Moreover, we demonstrated that eEF1Bγ binds the promoter region and the 3' UTR mRNA of the vimentin gene. These events contribute to localize the vimentin transcript and consequentially its translation, promoting a proper mitochondrial network. Methods: With the intent of identifying additional transcripts that complex with the eEF1Bγ protein, we performed a series of ribonucleoprotein immunoprecipitation (RIP) assays using a mitochondria-enriched heavy membrane (HM) fraction. Results: Among the eEF1Bγ complexed transcripts, we found the mRNA encoding the Che-1/AATF multifunctional protein. As reported by other research groups, we found the tumor suppressor p53 transcript complexed with the eEF1Bγ protein. Here, we show for the first time that eEF1Bγ binds not only Che-1 and p53 transcripts but also their promoters. Remarkably, we demonstrate that both the Che-1 transcript and its translated product localize also to the mitochondria and that eEF1Bγ depletion strongly perturbs the mitochondrial network and the correct localization of Che-1. In a doxorubicin (Dox)-induced DNA damage assay we show that eEF1Bγ depletion significantly decreases p53 protein accumulation and slightly impacts on Che-1 accumulation. Importantly, Che-1 and p53 proteins are components of the DNA damage response machinery that maintains genome integrity and prevents tumorigenesis. Conclusions: Our data support the notion that eEF1Bγ, besides its canonical role in translation, is an RNA-binding protein and a key player in cellular stress responses. We suggest for eEF1Bγ a role as primordial transcription/translation factor that links fundamental steps from transcription control to local translation.

Original languageEnglish
Article number146
JournalJournal of Experimental and Clinical Cancer Research
Volume35
Issue number1
DOIs
Publication statusPublished - Sep 17 2016

Fingerprint

Peptide Elongation Factors
p53 Genes
Vimentin
Proteins
DNA Damage
Mitochondria
Messenger RNA
Ribonucleoproteins
RNA-Binding Proteins
RNA Polymerase II
3' Untranslated Regions
Immunoprecipitation
Genetic Promoter Regions
Carcinogenesis
Transcription Factors
Genome

Keywords

  • AATF
  • Che-1
  • DNA damage
  • eEF1Bγ
  • Mitochondria
  • p53
  • POLR2C
  • RIP assay
  • RNA binding protein
  • Translation elongation factor

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Pisani, C., Onori, A., Gabanella, F., Delle Monache, F., Borreca, A., Ammassari-Teule, M., ... Corbi, N. (2016). EEF1Bγ binds the Che-1 and TP53 gene promoters and their transcripts. Journal of Experimental and Clinical Cancer Research, 35(1), [146]. https://doi.org/10.1186/s13046-016-0424-x

EEF1Bγ binds the Che-1 and TP53 gene promoters and their transcripts. / Pisani, Cinzia; Onori, Annalisa; Gabanella, Francesca; Delle Monache, Francesca; Borreca, Antonella; Ammassari-Teule, Martine; Fanciulli, Maurizio; Di Certo, Maria Grazia; Passananti, Claudio; Corbi, Nicoletta.

In: Journal of Experimental and Clinical Cancer Research, Vol. 35, No. 1, 146, 17.09.2016.

Research output: Contribution to journalArticle

Pisani, C, Onori, A, Gabanella, F, Delle Monache, F, Borreca, A, Ammassari-Teule, M, Fanciulli, M, Di Certo, MG, Passananti, C & Corbi, N 2016, 'EEF1Bγ binds the Che-1 and TP53 gene promoters and their transcripts', Journal of Experimental and Clinical Cancer Research, vol. 35, no. 1, 146. https://doi.org/10.1186/s13046-016-0424-x
Pisani C, Onori A, Gabanella F, Delle Monache F, Borreca A, Ammassari-Teule M et al. EEF1Bγ binds the Che-1 and TP53 gene promoters and their transcripts. Journal of Experimental and Clinical Cancer Research. 2016 Sep 17;35(1). 146. https://doi.org/10.1186/s13046-016-0424-x
Pisani, Cinzia ; Onori, Annalisa ; Gabanella, Francesca ; Delle Monache, Francesca ; Borreca, Antonella ; Ammassari-Teule, Martine ; Fanciulli, Maurizio ; Di Certo, Maria Grazia ; Passananti, Claudio ; Corbi, Nicoletta. / EEF1Bγ binds the Che-1 and TP53 gene promoters and their transcripts. In: Journal of Experimental and Clinical Cancer Research. 2016 ; Vol. 35, No. 1.
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AU - Borreca, Antonella

AU - Ammassari-Teule, Martine

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AB - Background: We have previously shown that the eukaryotic elongation factor subunit 1B gamma (eEF1Bγ) interacts with the RNA polymerase II (pol II) alpha-like subunit "C" (POLR2C), alone or complexed, in the pol II enzyme. Moreover, we demonstrated that eEF1Bγ binds the promoter region and the 3' UTR mRNA of the vimentin gene. These events contribute to localize the vimentin transcript and consequentially its translation, promoting a proper mitochondrial network. Methods: With the intent of identifying additional transcripts that complex with the eEF1Bγ protein, we performed a series of ribonucleoprotein immunoprecipitation (RIP) assays using a mitochondria-enriched heavy membrane (HM) fraction. Results: Among the eEF1Bγ complexed transcripts, we found the mRNA encoding the Che-1/AATF multifunctional protein. As reported by other research groups, we found the tumor suppressor p53 transcript complexed with the eEF1Bγ protein. Here, we show for the first time that eEF1Bγ binds not only Che-1 and p53 transcripts but also their promoters. Remarkably, we demonstrate that both the Che-1 transcript and its translated product localize also to the mitochondria and that eEF1Bγ depletion strongly perturbs the mitochondrial network and the correct localization of Che-1. In a doxorubicin (Dox)-induced DNA damage assay we show that eEF1Bγ depletion significantly decreases p53 protein accumulation and slightly impacts on Che-1 accumulation. Importantly, Che-1 and p53 proteins are components of the DNA damage response machinery that maintains genome integrity and prevents tumorigenesis. Conclusions: Our data support the notion that eEF1Bγ, besides its canonical role in translation, is an RNA-binding protein and a key player in cellular stress responses. We suggest for eEF1Bγ a role as primordial transcription/translation factor that links fundamental steps from transcription control to local translation.

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KW - Translation elongation factor

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