Effect of 17-β estradiol and epidermal growth factor on DNA and RNA labeling in astroglial cells during development, maturation and differentiation in culture

Nunzio Marletta, Davide Licciardello, Gian Francesco Cormaci, Maurizio Sabbatini, Antonio D'Assoro, Giorgia Venardi, Vittoria Spina-Purrello, Franca Stivala, Bianca Marchetti, Roberto Avola

Research output: Contribution to journalArticle

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Abstract

Growth factors stimulate astroglial and neuronal proliferation and differentiation in culture. Estrogens markedly influence astroglia, and are key factors participating in neurodegeneration. The aim of the present study was to investigate interactions between estradiol (E 2) and epidermal growth factor (EGF) during astroglia development, maturation and differentiation in culture. DNA or RNA labeling in 16 or 40 or 60 days in vitro (DIV) astrocyte cultures treated for 24 or 48 h with EGF and/or E 2 was evaluated. A significant increase in DNA labeling in 16 DIV astrocyte cultures treated for 24 h with EGF (5 ng/ml) and E 2 (1 nM) was found. EGF (5 or 10 ng/ml) addition in the last 24 h in 48 h E 2 (1 or 5 nM)-treated astrocyte cultures at 16 DIV caused a slight, but significant increase in DNA labeling. No differences in RNA labeling were observed in 16 DIV astrocyte cultures treated for 24 or 48 h with EGF (5 or 10 ng/ml) in the presence of E 2 (1 or 5 nM). A significant stimulation in DNA labeling was shown in 40 DIV astrocyte cultures treated for 48 h with E 2 (1 or 5 nM) in the presence of EGF (5 or 10 ng/ml) added in the last 24 h. In well differentiated astroglial cell cultures (60 DIV), DNA labeling was remarkably increased after 24 h treatment with 1 nM E 2 or 5 ng/ml EGF. Co-addition of 1 nM E 2 and 5 ng/ml EGF for 24 h reduced [methyl- 3H]thymidine incorporation, when data are compared to E 2- or EGF-treated cultures. Addition of EGF in the presence of E 2 for 48 h or only in the last 24 h caused a significant decrease of [methyl- 3H]thymidine incorporation in comparison with EGF-treated cultures at 60 DIV or with untreated cultures. Treatment of cultures for 24 h with EGF (5 or 10 ng/ml) alone or in combination with E 2 (1 or 5 nM) induced a strong increase of RNA labeling in 60 DIV astrocyte cultures. Addition for 48 h of E 2 (1 or 5 nM) or EGF (5 or 10 ng/ml) alone or in association stimulated significantly RNA labeling in astrocyte cultures at 60 DIV. When 60 DIV astrocyte cultures were treated for 48 h with E 2 (1 or 5 nM) in the presence of EGF (5 or 10 ng/ml) added only in the last 24 h, a potentiating effect of RNA labeling was observed. The above results suggest that interaction between growth factors and estrogens may contribute to regulate astroglia development, maturation and differentiation.

Original languageEnglish
Pages (from-to)1059-1072
Number of pages14
JournalMechanisms of Ageing and Development
Volume122
Issue number10
DOIs
Publication statusPublished - Jul 31 2001

Fingerprint

Epidermal Growth Factor
Labeling
Estradiol
Astrocytes
RNA
DNA
Thymidine
Intercellular Signaling Peptides and Proteins
Estrogens
In Vitro Techniques
Cell culture
Cell Culture Techniques

Keywords

  • Astroglial cell cultures
  • Development
  • Differentiation
  • DNA and RNA labeling
  • Epidermal growth factor
  • Estradiol
  • Maturation

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

Effect of 17-β estradiol and epidermal growth factor on DNA and RNA labeling in astroglial cells during development, maturation and differentiation in culture. / Marletta, Nunzio; Licciardello, Davide; Cormaci, Gian Francesco; Sabbatini, Maurizio; D'Assoro, Antonio; Venardi, Giorgia; Spina-Purrello, Vittoria; Stivala, Franca; Marchetti, Bianca; Avola, Roberto.

In: Mechanisms of Ageing and Development, Vol. 122, No. 10, 31.07.2001, p. 1059-1072.

Research output: Contribution to journalArticle

Marletta, N, Licciardello, D, Cormaci, GF, Sabbatini, M, D'Assoro, A, Venardi, G, Spina-Purrello, V, Stivala, F, Marchetti, B & Avola, R 2001, 'Effect of 17-β estradiol and epidermal growth factor on DNA and RNA labeling in astroglial cells during development, maturation and differentiation in culture', Mechanisms of Ageing and Development, vol. 122, no. 10, pp. 1059-1072. https://doi.org/10.1016/S0047-6374(01)00241-X
Marletta, Nunzio ; Licciardello, Davide ; Cormaci, Gian Francesco ; Sabbatini, Maurizio ; D'Assoro, Antonio ; Venardi, Giorgia ; Spina-Purrello, Vittoria ; Stivala, Franca ; Marchetti, Bianca ; Avola, Roberto. / Effect of 17-β estradiol and epidermal growth factor on DNA and RNA labeling in astroglial cells during development, maturation and differentiation in culture. In: Mechanisms of Ageing and Development. 2001 ; Vol. 122, No. 10. pp. 1059-1072.
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AU - Cormaci, Gian Francesco

AU - Sabbatini, Maurizio

AU - D'Assoro, Antonio

AU - Venardi, Giorgia

AU - Spina-Purrello, Vittoria

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N2 - Growth factors stimulate astroglial and neuronal proliferation and differentiation in culture. Estrogens markedly influence astroglia, and are key factors participating in neurodegeneration. The aim of the present study was to investigate interactions between estradiol (E 2) and epidermal growth factor (EGF) during astroglia development, maturation and differentiation in culture. DNA or RNA labeling in 16 or 40 or 60 days in vitro (DIV) astrocyte cultures treated for 24 or 48 h with EGF and/or E 2 was evaluated. A significant increase in DNA labeling in 16 DIV astrocyte cultures treated for 24 h with EGF (5 ng/ml) and E 2 (1 nM) was found. EGF (5 or 10 ng/ml) addition in the last 24 h in 48 h E 2 (1 or 5 nM)-treated astrocyte cultures at 16 DIV caused a slight, but significant increase in DNA labeling. No differences in RNA labeling were observed in 16 DIV astrocyte cultures treated for 24 or 48 h with EGF (5 or 10 ng/ml) in the presence of E 2 (1 or 5 nM). A significant stimulation in DNA labeling was shown in 40 DIV astrocyte cultures treated for 48 h with E 2 (1 or 5 nM) in the presence of EGF (5 or 10 ng/ml) added in the last 24 h. In well differentiated astroglial cell cultures (60 DIV), DNA labeling was remarkably increased after 24 h treatment with 1 nM E 2 or 5 ng/ml EGF. Co-addition of 1 nM E 2 and 5 ng/ml EGF for 24 h reduced [methyl- 3H]thymidine incorporation, when data are compared to E 2- or EGF-treated cultures. Addition of EGF in the presence of E 2 for 48 h or only in the last 24 h caused a significant decrease of [methyl- 3H]thymidine incorporation in comparison with EGF-treated cultures at 60 DIV or with untreated cultures. Treatment of cultures for 24 h with EGF (5 or 10 ng/ml) alone or in combination with E 2 (1 or 5 nM) induced a strong increase of RNA labeling in 60 DIV astrocyte cultures. Addition for 48 h of E 2 (1 or 5 nM) or EGF (5 or 10 ng/ml) alone or in association stimulated significantly RNA labeling in astrocyte cultures at 60 DIV. When 60 DIV astrocyte cultures were treated for 48 h with E 2 (1 or 5 nM) in the presence of EGF (5 or 10 ng/ml) added only in the last 24 h, a potentiating effect of RNA labeling was observed. The above results suggest that interaction between growth factors and estrogens may contribute to regulate astroglia development, maturation and differentiation.

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