Effect of a multistrain probiotic (Lactoflorene® Plus) on inflammatory parameters and microbiota composition in subjects with stress-related symptoms

Sara Soldi, Sara Carlotta Tagliacarne, Chiara Valsecchi, Simone Perna, Mariangela Rondanelli, Luigi Ziviani, Stefano Milleri, Ariella Annoni, Annamaria Castellazzi

Research output: Contribution to journalArticle

Abstract

Stress affects the immune system and intestinal microbiota composition and can lead to imbalance between pro- and anti-inflammatory cytokines or to uncontrolled production of cytokines. The effect of emotional stress on secretory IgA levels also indicates that stress decreases mucosal integrity. Our aim was to evaluate whether a probiotic product (Lactoflorene® Plus) can prevent alterations in the immune response associated with self-reported stress and microbiota composition. Healthy adult volunteers who self-reported psychological stress were enrolled and randomised into a placebo and a probiotic group. Salivary stress markers (α-amylase, cortisol, chromogranin A) and immunological parameters (sIgA, NK cell activity, IL-8, IL-10, TNF-α) in feces and the composition of intestinal microbiota were evaluated. Administration of the product did not exert a direct effect on the salivary stress markers or NK cell activity but did reduce abdominal pain and increase faecal IgA and IL-10 levels. The probiotic product induced a moderate increase in Bifidobacterium and Lactobacillus spp., as expected, and in Faecalibacterium spp., and decreased the size of the Dialister spp. and Escherichia and Shigella populations. Administration of the product helped protect the mucosal barrier by supporting the number of short-chain fatty acid producers and decreasing the load of potentially harmful bacteria, thus reducing intestinal inflammation and abdominal discomfort. ClinicalTrials.gov: NCT03234452.

Original languageEnglish
Article number100138
JournalNeurobiology of Stress
Volume10
DOIs
Publication statusPublished - Feb 1 2019

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Microbiota
Probiotics
Psychological Stress
Natural Killer Cells
Interleukin-10
Chemical analysis
Cytokines
Escherichia
Chromogranin A
Secretory Immunoglobulin A
Bifidobacterium
Shigella
Volatile Fatty Acids
Lactobacillus
Amylases
Interleukin-8
Feces
Immunoglobulin A
Abdominal Pain
Hydrocortisone

Keywords

  • Abdominal pain
  • BB-12
  • IgA
  • Immune response
  • LA-5
  • Stress

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

Cite this

Effect of a multistrain probiotic (Lactoflorene® Plus) on inflammatory parameters and microbiota composition in subjects with stress-related symptoms. / Soldi, Sara; Tagliacarne, Sara Carlotta; Valsecchi, Chiara; Perna, Simone; Rondanelli, Mariangela; Ziviani, Luigi; Milleri, Stefano; Annoni, Ariella; Castellazzi, Annamaria.

In: Neurobiology of Stress, Vol. 10, 100138, 01.02.2019.

Research output: Contribution to journalArticle

Soldi, S, Tagliacarne, SC, Valsecchi, C, Perna, S, Rondanelli, M, Ziviani, L, Milleri, S, Annoni, A & Castellazzi, A 2019, 'Effect of a multistrain probiotic (Lactoflorene® Plus) on inflammatory parameters and microbiota composition in subjects with stress-related symptoms', Neurobiology of Stress, vol. 10, 100138. https://doi.org/10.1016/j.ynstr.2018.11.001
Soldi, Sara ; Tagliacarne, Sara Carlotta ; Valsecchi, Chiara ; Perna, Simone ; Rondanelli, Mariangela ; Ziviani, Luigi ; Milleri, Stefano ; Annoni, Ariella ; Castellazzi, Annamaria. / Effect of a multistrain probiotic (Lactoflorene® Plus) on inflammatory parameters and microbiota composition in subjects with stress-related symptoms. In: Neurobiology of Stress. 2019 ; Vol. 10.
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