Effect of a novel immunosuppressant, ST1959, on the immune system and renal allograft survival in rats

Anna Pezzotta, Marilena Mister, Paolo Cravedi, Nadia Azzollini, Linda Cassis, Vito Ruggiero, Rita DeSantis, Paolo Carminati, Giuseppe Remuzzi, Marina Noris

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background. ST1959 is a 3,5-diaryl-s-triazole belonging to a novel class of contragestional agents with immunosuppressant activity. The aim of the present study was to investigate the effects of this drug on allogeneic immune response and on renal allograft survival in rats. Methods. One group of naive and one group of allosensitized Lewis rats received ST1959 (0.5 mg/kg/day for 6 days administered subcutaneously). The respective control groups received vehicle alone. At the end of treatment, all rats were killed and thymus, spleen, lymph nodes, bone marrow, and blood were harvested. Cell number, leukocyte subpopulations, and lymphocyte alloreactivity were evaluated. Three additional groups of Lewis rats received an allogeneic (Brown Norway [BN]) kidney transplant: two groups received ST1959 (0.5 mg/kg daily until death or for 6 days and then twice weekly), and the last one received vehicle. Results. In naive rats, ST1959 reduced the percentage of CD4+CD8+ (74.2±2.7%; vehicle, 89.1±1.1%; P-CD8- thymocytes (5.7±0.8% vs. 2.8±0.4%; P+ T cells (CD4+CD25 +) that was prevented by ST1959 treatment. Consistently, the alloreactivity of lymphocytes from naive and allosensitized animals treated with ST1959 was significantly lower than that of control rats. ST1959 (in both tested regimens) significantly prolonged renal allograft survival in comparison with vehicle (12.4±0.5 vs. 7.7±0.5 days; P

Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalTransplantation
Volume80
Issue number2
DOIs
Publication statusPublished - Jul 27 2005

Fingerprint

Immunosuppressive Agents
Allografts
Immune System
Kidney
Triazoles
Lymphocyte Subsets
Thymocytes
Norway
3-(2-ethylphenyl)-5-(3-methoxyphenyl)-1H-1,2,4-triazole
Thymus Gland
Leukocytes
Spleen
Cell Count
Lymph Nodes
Bone Marrow
Lymphocytes
T-Lymphocytes
Transplants
Control Groups
Pharmaceutical Preparations

Keywords

  • 3,5-Diaryl-s-triazole
  • Immunomodulatory effects
  • Immunosuppression
  • Renal allograft
  • ST1959

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Effect of a novel immunosuppressant, ST1959, on the immune system and renal allograft survival in rats. / Pezzotta, Anna; Mister, Marilena; Cravedi, Paolo; Azzollini, Nadia; Cassis, Linda; Ruggiero, Vito; DeSantis, Rita; Carminati, Paolo; Remuzzi, Giuseppe; Noris, Marina.

In: Transplantation, Vol. 80, No. 2, 27.07.2005, p. 231-236.

Research output: Contribution to journalArticle

Pezzotta, A, Mister, M, Cravedi, P, Azzollini, N, Cassis, L, Ruggiero, V, DeSantis, R, Carminati, P, Remuzzi, G & Noris, M 2005, 'Effect of a novel immunosuppressant, ST1959, on the immune system and renal allograft survival in rats', Transplantation, vol. 80, no. 2, pp. 231-236. https://doi.org/10.1097/01.TP.0000165434.89700.A7
Pezzotta, Anna ; Mister, Marilena ; Cravedi, Paolo ; Azzollini, Nadia ; Cassis, Linda ; Ruggiero, Vito ; DeSantis, Rita ; Carminati, Paolo ; Remuzzi, Giuseppe ; Noris, Marina. / Effect of a novel immunosuppressant, ST1959, on the immune system and renal allograft survival in rats. In: Transplantation. 2005 ; Vol. 80, No. 2. pp. 231-236.
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abstract = "Background. ST1959 is a 3,5-diaryl-s-triazole belonging to a novel class of contragestional agents with immunosuppressant activity. The aim of the present study was to investigate the effects of this drug on allogeneic immune response and on renal allograft survival in rats. Methods. One group of naive and one group of allosensitized Lewis rats received ST1959 (0.5 mg/kg/day for 6 days administered subcutaneously). The respective control groups received vehicle alone. At the end of treatment, all rats were killed and thymus, spleen, lymph nodes, bone marrow, and blood were harvested. Cell number, leukocyte subpopulations, and lymphocyte alloreactivity were evaluated. Three additional groups of Lewis rats received an allogeneic (Brown Norway [BN]) kidney transplant: two groups received ST1959 (0.5 mg/kg daily until death or for 6 days and then twice weekly), and the last one received vehicle. Results. In naive rats, ST1959 reduced the percentage of CD4+CD8+ (74.2±2.7{\%}; vehicle, 89.1±1.1{\%}; P-CD8- thymocytes (5.7±0.8{\%} vs. 2.8±0.4{\%}; P+ T cells (CD4+CD25 +) that was prevented by ST1959 treatment. Consistently, the alloreactivity of lymphocytes from naive and allosensitized animals treated with ST1959 was significantly lower than that of control rats. ST1959 (in both tested regimens) significantly prolonged renal allograft survival in comparison with vehicle (12.4±0.5 vs. 7.7±0.5 days; P",
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AU - Pezzotta, Anna

AU - Mister, Marilena

AU - Cravedi, Paolo

AU - Azzollini, Nadia

AU - Cassis, Linda

AU - Ruggiero, Vito

AU - DeSantis, Rita

AU - Carminati, Paolo

AU - Remuzzi, Giuseppe

AU - Noris, Marina

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N2 - Background. ST1959 is a 3,5-diaryl-s-triazole belonging to a novel class of contragestional agents with immunosuppressant activity. The aim of the present study was to investigate the effects of this drug on allogeneic immune response and on renal allograft survival in rats. Methods. One group of naive and one group of allosensitized Lewis rats received ST1959 (0.5 mg/kg/day for 6 days administered subcutaneously). The respective control groups received vehicle alone. At the end of treatment, all rats were killed and thymus, spleen, lymph nodes, bone marrow, and blood were harvested. Cell number, leukocyte subpopulations, and lymphocyte alloreactivity were evaluated. Three additional groups of Lewis rats received an allogeneic (Brown Norway [BN]) kidney transplant: two groups received ST1959 (0.5 mg/kg daily until death or for 6 days and then twice weekly), and the last one received vehicle. Results. In naive rats, ST1959 reduced the percentage of CD4+CD8+ (74.2±2.7%; vehicle, 89.1±1.1%; P-CD8- thymocytes (5.7±0.8% vs. 2.8±0.4%; P+ T cells (CD4+CD25 +) that was prevented by ST1959 treatment. Consistently, the alloreactivity of lymphocytes from naive and allosensitized animals treated with ST1959 was significantly lower than that of control rats. ST1959 (in both tested regimens) significantly prolonged renal allograft survival in comparison with vehicle (12.4±0.5 vs. 7.7±0.5 days; P

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