Effect of a regulatory mutation on the rat atrial natriuretic peptide gene transcription

Speranza Rubattu, Rosangela Giliberti, Paola De Paolis, Rosita Stanzione, Paola Spinsanti, Vanessa Venturelli, Massimo Volpe

Research output: Contribution to journalArticle

Abstract

To investigate the functional relevance of a regulatory mutation affecting the enhancer element PEA2 of the rat ANP gene we transfected rat cardiomyocytes and aortic endothelial cells with either the mutant or the wild-type ANP promoter construct (-683 +54) and performed CAT assays both at baseline and in response to Phenylephrine and Angiotensin II. In the myocardial cells we also determined the DNA/nuclear protein interaction through electrophoretic mobility shift assay. These studies showed a significantly lower degree of ANP transcription in the presence of the mutant PEA2 site, thus demonstrating its functional significance and the biological relevance of ANP gene structural alterations.

Original languageEnglish
Pages (from-to)555-560
Number of pages6
JournalPeptides
Volume23
Issue number3
DOIs
Publication statusPublished - 2002

Keywords

  • ANP promoter
  • Atrial natriuretic peptide
  • Gene mutation
  • PEA2

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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