Effect of a topical treatment in organotypic culture of human breast skin after exposure to gamma-rays

Nicoletta Gagliano, M. Bedoni, G. Mantovani, M. Chiriva-Internati, D. Castelli, C. Torri, E. Donetti

Research output: Contribution to journalArticlepeer-review


The early radiation of epidermal reactions can lead to healing of the lesion or radiation necrosis. There is no general agreement for either the prevention and/or treatment of radiation skin response, also as little is known about the immediate phases of this phenomenon. We investigated the early effects exerted by Healing and Wound Emulsion (HWE) on human skin response after ionizing radiation. Epidermal morphology, Heat Shock Protein (HSP) 70, and Transforming Growth Factor-β1 (TGF-β1) gene expression were investigated in organotypic human skin cultures undergoing a double dose of gamma-rays (2 Gy). HSP70 gene expression tended to be induced in the HWE group 6 hours after cream administration and was significantly up-regulated after 48 hours, when epidermal morphological alterations were evident. TGF-β1 seems not affected in cream treated samples. HWE may stimulate skin to mount an early defensive response against damage induced by gamma rays.

Original languageEnglish
Pages (from-to)283-289
Number of pages7
JournalEuropean journal of histochemistry : EJH
Issue number4
Publication statusPublished - Oct 2007


  • Epidermis
  • HSP70
  • Ionizing radiations
  • Radiodermatitis
  • TGF-β1

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Plant Science
  • Biophysics
  • Cell Biology
  • Developmental Biology
  • Molecular Biology
  • Anatomy
  • Histology


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