TY - JOUR
T1 - Effect of activin a on tumor necrosis factor-α/intercellular adhesion molecule-1 pathway in endometrial stromal cells
AU - Mangioni, Silvia
AU - Viganò, Paola
AU - Florio, Pasquale
AU - Borghi, Orietta
AU - Vignali, Michele
AU - Petraglia, Felice
AU - Blasio, Anna Maria Di
PY - 2005/12/1
Y1 - 2005/12/1
N2 - Objective[s]: Activin A and inhibin A are growth factors expressed by human endometrium involved in the control of endometrial functions. In the present study we investigated the effects of activin A and inhibin A in modulating the tumor necrosis factor (TNF)-α/intercellular adhesion molecule (ICAM)-1 system in cultured human endometrial stromal cells. Study design: Endometrial samples were obtained from 34 reproductive age women undergoing laparoscopy for benign ovarian cysts or infertility. Endometrial stromal cells were cultured and soluble ICAM-1 and TNF-α were measured in cell-free supernatants following treatment with or without activin A or inhibin A. Cell surface ICAM-1 was assayed by flow cytometry by staining endometrial cells with specific monoclonal antibodies. Results: Activin A and inhibin A did not influence either the expression of cell surface ICAM-1 or soluble ICAM-1 shedding by cultured endometrial cells. On the other hand, TNF-α secretion significantly increased in presence of activin A but not of inhibin A. Conclusions: Since TNF-α modulates several endometrial processes such as menstruation, proliferation, apoptosis, implantation and decidualization, an effect of activin A in the physiological control of endometrium is further supported by the present data.
AB - Objective[s]: Activin A and inhibin A are growth factors expressed by human endometrium involved in the control of endometrial functions. In the present study we investigated the effects of activin A and inhibin A in modulating the tumor necrosis factor (TNF)-α/intercellular adhesion molecule (ICAM)-1 system in cultured human endometrial stromal cells. Study design: Endometrial samples were obtained from 34 reproductive age women undergoing laparoscopy for benign ovarian cysts or infertility. Endometrial stromal cells were cultured and soluble ICAM-1 and TNF-α were measured in cell-free supernatants following treatment with or without activin A or inhibin A. Cell surface ICAM-1 was assayed by flow cytometry by staining endometrial cells with specific monoclonal antibodies. Results: Activin A and inhibin A did not influence either the expression of cell surface ICAM-1 or soluble ICAM-1 shedding by cultured endometrial cells. On the other hand, TNF-α secretion significantly increased in presence of activin A but not of inhibin A. Conclusions: Since TNF-α modulates several endometrial processes such as menstruation, proliferation, apoptosis, implantation and decidualization, an effect of activin A in the physiological control of endometrium is further supported by the present data.
KW - Activin A
KW - Endometrium
KW - ICAM-1
KW - TNF-α
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U2 - 10.1016/j.ejogrb.2005.03.017
DO - 10.1016/j.ejogrb.2005.03.017
M3 - Article
C2 - 15893868
AN - SCOPUS:28444460252
VL - 123
SP - 218
EP - 223
JO - European Journal of Obstetrics, Gynecology and Reproductive Biology
JF - European Journal of Obstetrics, Gynecology and Reproductive Biology
SN - 0028-2243
IS - 2
ER -