Effect of activin a on tumor necrosis factor-α/intercellular adhesion molecule-1 pathway in endometrial stromal cells

Silvia Mangioni, Paola Viganò, Pasquale Florio, Orietta Borghi, Michele Vignali, Felice Petraglia, Anna Maria Di Blasio

Research output: Contribution to journalArticlepeer-review

Abstract

Objective[s]: Activin A and inhibin A are growth factors expressed by human endometrium involved in the control of endometrial functions. In the present study we investigated the effects of activin A and inhibin A in modulating the tumor necrosis factor (TNF)-α/intercellular adhesion molecule (ICAM)-1 system in cultured human endometrial stromal cells. Study design: Endometrial samples were obtained from 34 reproductive age women undergoing laparoscopy for benign ovarian cysts or infertility. Endometrial stromal cells were cultured and soluble ICAM-1 and TNF-α were measured in cell-free supernatants following treatment with or without activin A or inhibin A. Cell surface ICAM-1 was assayed by flow cytometry by staining endometrial cells with specific monoclonal antibodies. Results: Activin A and inhibin A did not influence either the expression of cell surface ICAM-1 or soluble ICAM-1 shedding by cultured endometrial cells. On the other hand, TNF-α secretion significantly increased in presence of activin A but not of inhibin A. Conclusions: Since TNF-α modulates several endometrial processes such as menstruation, proliferation, apoptosis, implantation and decidualization, an effect of activin A in the physiological control of endometrium is further supported by the present data.

Original languageEnglish
Pages (from-to)218-223
Number of pages6
JournalEuropean Journal of Obstetrics and Gynecology and Reproductive Biology
Volume123
Issue number2
DOIs
Publication statusPublished - Dec 1 2005

Keywords

  • Activin A
  • Endometrium
  • ICAM-1
  • TNF-α

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

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