Abstract
ADP-ribosyl transferase (ADPRT) is a nuclear enzyme activated by single (SSB) and double (DSB) DNA strand breaks which synthesizes ADP-ribose homopolymers bound to nuclear proteins. The physiological role of ADPRT is still poorly understood, although it has been suggested that it plays a role in DNA repair and cell proliferation and differentiation. Excessive activation of the enzyme can exhaust intracellular NAD+ and ATP pools, thereby contributing to the so-called suicide response of extensively damaged cells. We asked whether ADPRT inhibitors, such as 3-aminobenzamide (3-ABA) and nicotinamide (NAM), would prevent the suicide response of human lymophocyte damaged by gamma rays in the same way as in lymphocytes damaged by oxygen radicals, taking into account that cells damaged by both agents show increased ADPRT activity and DNA strand breaks.
| Original language | English |
|---|---|
| Pages (from-to) | 446-447 |
| Number of pages | 2 |
| Journal | Annals of the New York Academy of Sciences |
| Volume | 551 |
| Publication status | Published - 1988 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
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Effect of ADP-ribosyl transferase inhibitors on the survival of human lymphocytes after exposure to different DNA-damaging agents. / Franceschi, C.; Marini, M.; Zunica, G.; Monti, D.; Cossarizza, A.; Bologni, A.; Gatti, C.; Brunelli, M. A.
In: Annals of the New York Academy of Sciences, Vol. 551, 1988, p. 446-447.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Effect of ADP-ribosyl transferase inhibitors on the survival of human lymphocytes after exposure to different DNA-damaging agents
AU - Franceschi, C.
AU - Marini, M.
AU - Zunica, G.
AU - Monti, D.
AU - Cossarizza, A.
AU - Bologni, A.
AU - Gatti, C.
AU - Brunelli, M. A.
PY - 1988
Y1 - 1988
N2 - ADP-ribosyl transferase (ADPRT) is a nuclear enzyme activated by single (SSB) and double (DSB) DNA strand breaks which synthesizes ADP-ribose homopolymers bound to nuclear proteins. The physiological role of ADPRT is still poorly understood, although it has been suggested that it plays a role in DNA repair and cell proliferation and differentiation. Excessive activation of the enzyme can exhaust intracellular NAD+ and ATP pools, thereby contributing to the so-called suicide response of extensively damaged cells. We asked whether ADPRT inhibitors, such as 3-aminobenzamide (3-ABA) and nicotinamide (NAM), would prevent the suicide response of human lymophocyte damaged by gamma rays in the same way as in lymphocytes damaged by oxygen radicals, taking into account that cells damaged by both agents show increased ADPRT activity and DNA strand breaks.
AB - ADP-ribosyl transferase (ADPRT) is a nuclear enzyme activated by single (SSB) and double (DSB) DNA strand breaks which synthesizes ADP-ribose homopolymers bound to nuclear proteins. The physiological role of ADPRT is still poorly understood, although it has been suggested that it plays a role in DNA repair and cell proliferation and differentiation. Excessive activation of the enzyme can exhaust intracellular NAD+ and ATP pools, thereby contributing to the so-called suicide response of extensively damaged cells. We asked whether ADPRT inhibitors, such as 3-aminobenzamide (3-ABA) and nicotinamide (NAM), would prevent the suicide response of human lymophocyte damaged by gamma rays in the same way as in lymphocytes damaged by oxygen radicals, taking into account that cells damaged by both agents show increased ADPRT activity and DNA strand breaks.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024149649&partnerID=8YFLogxK
M3 - Article
C2 - 2977536
AN - SCOPUS:0024149649
VL - 551
SP - 446
EP - 447
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
SN - 0077-8923
ER -