Effect of ADP-ribosyl transferase inhibitors on the survival of human lymphocytes after exposure to different DNA-damaging agents

C. Franceschi, M. Marini, G. Zunica, D. Monti, A. Cossarizza, A. Bologni, C. Gatti, M. A. Brunelli

Research output: Contribution to journalArticle

Abstract

ADP-ribosyl transferase (ADPRT) is a nuclear enzyme activated by single (SSB) and double (DSB) DNA strand breaks which synthesizes ADP-ribose homopolymers bound to nuclear proteins. The physiological role of ADPRT is still poorly understood, although it has been suggested that it plays a role in DNA repair and cell proliferation and differentiation. Excessive activation of the enzyme can exhaust intracellular NAD+ and ATP pools, thereby contributing to the so-called suicide response of extensively damaged cells. We asked whether ADPRT inhibitors, such as 3-aminobenzamide (3-ABA) and nicotinamide (NAM), would prevent the suicide response of human lymophocyte damaged by gamma rays in the same way as in lymphocytes damaged by oxygen radicals, taking into account that cells damaged by both agents show increased ADPRT activity and DNA strand breaks.

Original languageEnglish
Pages (from-to)446-447
Number of pages2
JournalAnnals of the New York Academy of Sciences
Volume551
Publication statusPublished - 1988

Fingerprint

Lymphocytes
Transferases
Adenosine Diphosphate
DNA
Suicide
Adenosine Diphosphate Ribose
DNA Breaks
Enzyme Activation
Niacinamide
Double-Stranded DNA Breaks
Gamma Rays
Cell proliferation
Enzymes
Nuclear Proteins
Homopolymerization
DNA Repair
Gamma rays
NAD
Cell Differentiation
Reactive Oxygen Species

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Effect of ADP-ribosyl transferase inhibitors on the survival of human lymphocytes after exposure to different DNA-damaging agents. / Franceschi, C.; Marini, M.; Zunica, G.; Monti, D.; Cossarizza, A.; Bologni, A.; Gatti, C.; Brunelli, M. A.

In: Annals of the New York Academy of Sciences, Vol. 551, 1988, p. 446-447.

Research output: Contribution to journalArticle

Franceschi, C. ; Marini, M. ; Zunica, G. ; Monti, D. ; Cossarizza, A. ; Bologni, A. ; Gatti, C. ; Brunelli, M. A. / Effect of ADP-ribosyl transferase inhibitors on the survival of human lymphocytes after exposure to different DNA-damaging agents. In: Annals of the New York Academy of Sciences. 1988 ; Vol. 551. pp. 446-447.
@article{00e6238ef6984809abe2df5322677832,
title = "Effect of ADP-ribosyl transferase inhibitors on the survival of human lymphocytes after exposure to different DNA-damaging agents",
abstract = "ADP-ribosyl transferase (ADPRT) is a nuclear enzyme activated by single (SSB) and double (DSB) DNA strand breaks which synthesizes ADP-ribose homopolymers bound to nuclear proteins. The physiological role of ADPRT is still poorly understood, although it has been suggested that it plays a role in DNA repair and cell proliferation and differentiation. Excessive activation of the enzyme can exhaust intracellular NAD+ and ATP pools, thereby contributing to the so-called suicide response of extensively damaged cells. We asked whether ADPRT inhibitors, such as 3-aminobenzamide (3-ABA) and nicotinamide (NAM), would prevent the suicide response of human lymophocyte damaged by gamma rays in the same way as in lymphocytes damaged by oxygen radicals, taking into account that cells damaged by both agents show increased ADPRT activity and DNA strand breaks.",
author = "C. Franceschi and M. Marini and G. Zunica and D. Monti and A. Cossarizza and A. Bologni and C. Gatti and Brunelli, {M. A.}",
year = "1988",
language = "English",
volume = "551",
pages = "446--447",
journal = "Annals of the New York Academy of Sciences",
issn = "0077-8923",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Effect of ADP-ribosyl transferase inhibitors on the survival of human lymphocytes after exposure to different DNA-damaging agents

AU - Franceschi, C.

AU - Marini, M.

AU - Zunica, G.

AU - Monti, D.

AU - Cossarizza, A.

AU - Bologni, A.

AU - Gatti, C.

AU - Brunelli, M. A.

PY - 1988

Y1 - 1988

N2 - ADP-ribosyl transferase (ADPRT) is a nuclear enzyme activated by single (SSB) and double (DSB) DNA strand breaks which synthesizes ADP-ribose homopolymers bound to nuclear proteins. The physiological role of ADPRT is still poorly understood, although it has been suggested that it plays a role in DNA repair and cell proliferation and differentiation. Excessive activation of the enzyme can exhaust intracellular NAD+ and ATP pools, thereby contributing to the so-called suicide response of extensively damaged cells. We asked whether ADPRT inhibitors, such as 3-aminobenzamide (3-ABA) and nicotinamide (NAM), would prevent the suicide response of human lymophocyte damaged by gamma rays in the same way as in lymphocytes damaged by oxygen radicals, taking into account that cells damaged by both agents show increased ADPRT activity and DNA strand breaks.

AB - ADP-ribosyl transferase (ADPRT) is a nuclear enzyme activated by single (SSB) and double (DSB) DNA strand breaks which synthesizes ADP-ribose homopolymers bound to nuclear proteins. The physiological role of ADPRT is still poorly understood, although it has been suggested that it plays a role in DNA repair and cell proliferation and differentiation. Excessive activation of the enzyme can exhaust intracellular NAD+ and ATP pools, thereby contributing to the so-called suicide response of extensively damaged cells. We asked whether ADPRT inhibitors, such as 3-aminobenzamide (3-ABA) and nicotinamide (NAM), would prevent the suicide response of human lymophocyte damaged by gamma rays in the same way as in lymphocytes damaged by oxygen radicals, taking into account that cells damaged by both agents show increased ADPRT activity and DNA strand breaks.

UR - http://www.scopus.com/inward/record.url?scp=0024149649&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024149649&partnerID=8YFLogxK

M3 - Article

C2 - 2977536

AN - SCOPUS:0024149649

VL - 551

SP - 446

EP - 447

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

SN - 0077-8923

ER -