Effect of aminoguanidine in ligature-induced periodontitis in rats

R. Di Paola, S. Marzocco, E. Mazzon, F. Dattola, F. Rotondo, D. Britti, M. De Majo, T. Genovese, S. Cuzzocrea

Research output: Contribution to journalArticlepeer-review


The role of nitric oxide and reactive oxygen species is well-demonstrated in inflammation. In this study, we evaluated the effect of aminoguanidine, a nitric oxide synthase inhibitor, in a rat model of periodontitis. We induced periodontitis in rats by placing a piece of 2/0 braided silk around the lower left 1st molar. At day 8, the gingivomucosal tissue encircling the mandibular 1st molar was removed for biochemical and histological analysis. Ligation significantly increased inducible nitric oxide synthase activity and expression, and damaged tissue revealed increased neutrophil infiltration, lipid peroxidation, and positive staining for nitrotyrosine formation and poly (ADP-ribose) polymerase activation. Ligation significantly increased Evans blue extravasation in gingivomucosal tissue and alveolar bone destruction. Aminoguanidine (100 mg/kg i.p., daily for 8 days) treatment significantly reduced all these inflammatory parameters, indicating that it protects against the tissue damage associated with periodontitis by reducing nitric oxide production and oxidative stress.

Original languageEnglish
Pages (from-to)343-348
Number of pages6
JournalJournal of Dental Research
Issue number4
Publication statusPublished - Apr 2004


  • Alveolar bone loss
  • Aminoguanidine
  • Inducible nitric oxide synthase
  • Periodontitis
  • Poly (ADP-ribose) polymerase

ASJC Scopus subject areas

  • Dentistry(all)


Dive into the research topics of 'Effect of aminoguanidine in ligature-induced periodontitis in rats'. Together they form a unique fingerprint.

Cite this