Effect of aminoguanidine in ligature-induced periodontitis in rats

R. Di Paola, S. Marzocco, E. Mazzon, F. Dattola, F. Rotondo, D. Britti, M. De Majo, T. Genovese, S. Cuzzocrea

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

The role of nitric oxide and reactive oxygen species is well-demonstrated in inflammation. In this study, we evaluated the effect of aminoguanidine, a nitric oxide synthase inhibitor, in a rat model of periodontitis. We induced periodontitis in rats by placing a piece of 2/0 braided silk around the lower left 1st molar. At day 8, the gingivomucosal tissue encircling the mandibular 1st molar was removed for biochemical and histological analysis. Ligation significantly increased inducible nitric oxide synthase activity and expression, and damaged tissue revealed increased neutrophil infiltration, lipid peroxidation, and positive staining for nitrotyrosine formation and poly (ADP-ribose) polymerase activation. Ligation significantly increased Evans blue extravasation in gingivomucosal tissue and alveolar bone destruction. Aminoguanidine (100 mg/kg i.p., daily for 8 days) treatment significantly reduced all these inflammatory parameters, indicating that it protects against the tissue damage associated with periodontitis by reducing nitric oxide production and oxidative stress.

Original languageEnglish
Pages (from-to)343-348
Number of pages6
JournalJournal of Dental Research
Volume83
Issue number4
Publication statusPublished - Apr 2004

Fingerprint

Periodontitis
Ligation
Nitric Oxide
Evans Blue
Silk
Poly(ADP-ribose) Polymerases
Neutrophil Infiltration
Nitric Oxide Synthase Type II
Nitric Oxide Synthase
Lipid Peroxidation
Reactive Oxygen Species
Oxidative Stress
Staining and Labeling
Inflammation
Bone and Bones
pimagedine

Keywords

  • Alveolar bone loss
  • Aminoguanidine
  • Inducible nitric oxide synthase
  • Periodontitis
  • Poly (ADP-ribose) polymerase

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Di Paola, R., Marzocco, S., Mazzon, E., Dattola, F., Rotondo, F., Britti, D., ... Cuzzocrea, S. (2004). Effect of aminoguanidine in ligature-induced periodontitis in rats. Journal of Dental Research, 83(4), 343-348.

Effect of aminoguanidine in ligature-induced periodontitis in rats. / Di Paola, R.; Marzocco, S.; Mazzon, E.; Dattola, F.; Rotondo, F.; Britti, D.; De Majo, M.; Genovese, T.; Cuzzocrea, S.

In: Journal of Dental Research, Vol. 83, No. 4, 04.2004, p. 343-348.

Research output: Contribution to journalArticle

Di Paola, R, Marzocco, S, Mazzon, E, Dattola, F, Rotondo, F, Britti, D, De Majo, M, Genovese, T & Cuzzocrea, S 2004, 'Effect of aminoguanidine in ligature-induced periodontitis in rats', Journal of Dental Research, vol. 83, no. 4, pp. 343-348.
Di Paola R, Marzocco S, Mazzon E, Dattola F, Rotondo F, Britti D et al. Effect of aminoguanidine in ligature-induced periodontitis in rats. Journal of Dental Research. 2004 Apr;83(4):343-348.
Di Paola, R. ; Marzocco, S. ; Mazzon, E. ; Dattola, F. ; Rotondo, F. ; Britti, D. ; De Majo, M. ; Genovese, T. ; Cuzzocrea, S. / Effect of aminoguanidine in ligature-induced periodontitis in rats. In: Journal of Dental Research. 2004 ; Vol. 83, No. 4. pp. 343-348.
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