Abstract
In HIV-1-infected cell cultures, a relatively low concentration (5 μg/ml) of monoclonal antibody (mAb) against HIV-1-transactivating Tat protein was an efficient inhibitor of HIV-1 replication both in HIV-1(IIIB)-infected Jurkat cell and peripheral blood mononuclear cell (PBMC) cultures and significantly reduced the expression of a Tat-responsive CAT-reporter construct in HIV- 1(IIIB)-infected Jurkat cells. Anti-Tat mAb also caused a significant reduction and a consistent delay in HIV-1 replication when added to PBMCs from HIV-1-infected patients cocultivated with phytohemagglutinin (PHA)stimulated normal PBMCs. These data indicate that an autocrine-paracrine loop sustained by extracellular Tat protein, which is actively released by HIV-1-infected cells, may affect HIV-1 replication in cell cultures in vitro. An inverse relationship between natural anti-Tat antibody levels and p24 antigenemia was demonstrated by retrospective analysis of serial serum samples obtained from 10 HIV-1-seropositive hemophiliac patients followed over a 7-9-year period. This datum points to a possible influence of anti- Tat antibody on the progression of HIV-1 disease in vivo. These findings have strong implications for Tat protein as a possible target for specific immunotherapy in HIV-1-infected patients.
Original language | English |
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Pages (from-to) | 408-416 |
Number of pages | 9 |
Journal | Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology |
Volume | 10 |
Issue number | 4 |
Publication status | Published - 1995 |
Keywords
- anti-Tat antibody
- Hemophiliac patients
- HIV-1
- p24 Antigen
- Tat protein
ASJC Scopus subject areas
- Immunology
- Virology
- Immunology and Allergy