Abstract
In HIV-1-infected cell cultures, a relatively low concentration (5 μg/ml) of monoclonal antibody (mAb) against HIV-1-transactivating Tat protein was an efficient inhibitor of HIV-1 replication both in HIV-1(IIIB)-infected Jurkat cell and peripheral blood mononuclear cell (PBMC) cultures and significantly reduced the expression of a Tat-responsive CAT-reporter construct in HIV- 1(IIIB)-infected Jurkat cells. Anti-Tat mAb also caused a significant reduction and a consistent delay in HIV-1 replication when added to PBMCs from HIV-1-infected patients cocultivated with phytohemagglutinin (PHA)stimulated normal PBMCs. These data indicate that an autocrine-paracrine loop sustained by extracellular Tat protein, which is actively released by HIV-1-infected cells, may affect HIV-1 replication in cell cultures in vitro. An inverse relationship between natural anti-Tat antibody levels and p24 antigenemia was demonstrated by retrospective analysis of serial serum samples obtained from 10 HIV-1-seropositive hemophiliac patients followed over a 7-9-year period. This datum points to a possible influence of anti- Tat antibody on the progression of HIV-1 disease in vivo. These findings have strong implications for Tat protein as a possible target for specific immunotherapy in HIV-1-infected patients.
| Original language | English |
|---|---|
| Pages (from-to) | 408-416 |
| Number of pages | 9 |
| Journal | Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology |
| Volume | 10 |
| Issue number | 4 |
| Publication status | Published - 1995 |
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Keywords
- anti-Tat antibody
- Hemophiliac patients
- HIV-1
- p24 Antigen
- Tat protein
ASJC Scopus subject areas
- Immunology
- Virology
- Immunology and Allergy
Cite this
Effect of antibody to HIV-1 Tat protein on viral replication in vitro and progression of HIV-1 disease in vivo. / Re, M. C.; Furlini, G.; Vignoli, M.; Ramazzotti, E.; Roderigo, G.; De Rosa, V.; Zauli, G.; Lolli, S.; Capitani, S.; La Placa, M.
In: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, Vol. 10, No. 4, 1995, p. 408-416.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of antibody to HIV-1 Tat protein on viral replication in vitro and progression of HIV-1 disease in vivo
AU - Re, M. C.
AU - Furlini, G.
AU - Vignoli, M.
AU - Ramazzotti, E.
AU - Roderigo, G.
AU - De Rosa, V.
AU - Zauli, G.
AU - Lolli, S.
AU - Capitani, S.
AU - La Placa, M.
PY - 1995
Y1 - 1995
N2 - In HIV-1-infected cell cultures, a relatively low concentration (5 μg/ml) of monoclonal antibody (mAb) against HIV-1-transactivating Tat protein was an efficient inhibitor of HIV-1 replication both in HIV-1(IIIB)-infected Jurkat cell and peripheral blood mononuclear cell (PBMC) cultures and significantly reduced the expression of a Tat-responsive CAT-reporter construct in HIV- 1(IIIB)-infected Jurkat cells. Anti-Tat mAb also caused a significant reduction and a consistent delay in HIV-1 replication when added to PBMCs from HIV-1-infected patients cocultivated with phytohemagglutinin (PHA)stimulated normal PBMCs. These data indicate that an autocrine-paracrine loop sustained by extracellular Tat protein, which is actively released by HIV-1-infected cells, may affect HIV-1 replication in cell cultures in vitro. An inverse relationship between natural anti-Tat antibody levels and p24 antigenemia was demonstrated by retrospective analysis of serial serum samples obtained from 10 HIV-1-seropositive hemophiliac patients followed over a 7-9-year period. This datum points to a possible influence of anti- Tat antibody on the progression of HIV-1 disease in vivo. These findings have strong implications for Tat protein as a possible target for specific immunotherapy in HIV-1-infected patients.
AB - In HIV-1-infected cell cultures, a relatively low concentration (5 μg/ml) of monoclonal antibody (mAb) against HIV-1-transactivating Tat protein was an efficient inhibitor of HIV-1 replication both in HIV-1(IIIB)-infected Jurkat cell and peripheral blood mononuclear cell (PBMC) cultures and significantly reduced the expression of a Tat-responsive CAT-reporter construct in HIV- 1(IIIB)-infected Jurkat cells. Anti-Tat mAb also caused a significant reduction and a consistent delay in HIV-1 replication when added to PBMCs from HIV-1-infected patients cocultivated with phytohemagglutinin (PHA)stimulated normal PBMCs. These data indicate that an autocrine-paracrine loop sustained by extracellular Tat protein, which is actively released by HIV-1-infected cells, may affect HIV-1 replication in cell cultures in vitro. An inverse relationship between natural anti-Tat antibody levels and p24 antigenemia was demonstrated by retrospective analysis of serial serum samples obtained from 10 HIV-1-seropositive hemophiliac patients followed over a 7-9-year period. This datum points to a possible influence of anti- Tat antibody on the progression of HIV-1 disease in vivo. These findings have strong implications for Tat protein as a possible target for specific immunotherapy in HIV-1-infected patients.
KW - anti-Tat antibody
KW - Hemophiliac patients
KW - HIV-1
KW - p24 Antigen
KW - Tat protein
UR - http://www.scopus.com/inward/record.url?scp=0028785665&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028785665&partnerID=8YFLogxK
M3 - Article
C2 - 7583436
AN - SCOPUS:0028785665
VL - 10
SP - 408
EP - 416
JO - Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
JF - Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology
SN - 1077-9450
IS - 4
ER -