Effect of antibody to HIV-1 Tat protein on viral replication in vitro and progression of HIV-1 disease in vivo

M. C. Re, G. Furlini, M. Vignoli, E. Ramazzotti, G. Roderigo, V. De Rosa, G. Zauli, S. Lolli, S. Capitani, M. La Placa

Research output: Contribution to journalArticle

Abstract

In HIV-1-infected cell cultures, a relatively low concentration (5 μg/ml) of monoclonal antibody (mAb) against HIV-1-transactivating Tat protein was an efficient inhibitor of HIV-1 replication both in HIV-1(IIIB)-infected Jurkat cell and peripheral blood mononuclear cell (PBMC) cultures and significantly reduced the expression of a Tat-responsive CAT-reporter construct in HIV- 1(IIIB)-infected Jurkat cells. Anti-Tat mAb also caused a significant reduction and a consistent delay in HIV-1 replication when added to PBMCs from HIV-1-infected patients cocultivated with phytohemagglutinin (PHA)stimulated normal PBMCs. These data indicate that an autocrine-paracrine loop sustained by extracellular Tat protein, which is actively released by HIV-1-infected cells, may affect HIV-1 replication in cell cultures in vitro. An inverse relationship between natural anti-Tat antibody levels and p24 antigenemia was demonstrated by retrospective analysis of serial serum samples obtained from 10 HIV-1-seropositive hemophiliac patients followed over a 7-9-year period. This datum points to a possible influence of anti- Tat antibody on the progression of HIV-1 disease in vivo. These findings have strong implications for Tat protein as a possible target for specific immunotherapy in HIV-1-infected patients.

Original languageEnglish
Pages (from-to)408-416
Number of pages9
JournalJournal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume10
Issue number4
Publication statusPublished - 1995

Fingerprint

Human Immunodeficiency Virus tat Gene Products
HIV-1
Antibodies
tat Gene Products
Jurkat Cells
Cell Culture Techniques
In Vitro Techniques
Anti-Idiotypic Antibodies
Monoclonal Antibodies
Phytohemagglutinins
Immunotherapy

Keywords

  • anti-Tat antibody
  • Hemophiliac patients
  • HIV-1
  • p24 Antigen
  • Tat protein

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Immunology and Allergy

Cite this

Re, M. C., Furlini, G., Vignoli, M., Ramazzotti, E., Roderigo, G., De Rosa, V., ... La Placa, M. (1995). Effect of antibody to HIV-1 Tat protein on viral replication in vitro and progression of HIV-1 disease in vivo. Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, 10(4), 408-416.

Effect of antibody to HIV-1 Tat protein on viral replication in vitro and progression of HIV-1 disease in vivo. / Re, M. C.; Furlini, G.; Vignoli, M.; Ramazzotti, E.; Roderigo, G.; De Rosa, V.; Zauli, G.; Lolli, S.; Capitani, S.; La Placa, M.

In: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, Vol. 10, No. 4, 1995, p. 408-416.

Research output: Contribution to journalArticle

Re, MC, Furlini, G, Vignoli, M, Ramazzotti, E, Roderigo, G, De Rosa, V, Zauli, G, Lolli, S, Capitani, S & La Placa, M 1995, 'Effect of antibody to HIV-1 Tat protein on viral replication in vitro and progression of HIV-1 disease in vivo', Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, vol. 10, no. 4, pp. 408-416.
Re, M. C. ; Furlini, G. ; Vignoli, M. ; Ramazzotti, E. ; Roderigo, G. ; De Rosa, V. ; Zauli, G. ; Lolli, S. ; Capitani, S. ; La Placa, M. / Effect of antibody to HIV-1 Tat protein on viral replication in vitro and progression of HIV-1 disease in vivo. In: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology. 1995 ; Vol. 10, No. 4. pp. 408-416.
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