Abstract
NADPH-oxidase is an enzyme responsible for reactive oxygen species production, and inhibition of this enzyme represents an attractive therapeutic target for the treatment of many diseases. The aim of this study was to investigate the effects of Apocynin, NADPH-oxidase inhibitor, in the modulation of secondary injury in the spinal cord. The injury was induced by application of vascular clips to the dura via a four-level T5T8 laminectomy in mice. Treatment with Apocynin 1 and 6 h after the trauma significantly decreased (1) the degree of spinal cord inflammation and tissue injury, (2) neutrophil infiltration, (3) adhesion molecule expression, (4) nuclear transcription factorκB expression, (5) nitrotyrosine and poly-ADP-ribose formation, (6) pro-inflammatory cytokines production, (7) apoptosis and (8) mitogen-activated protein kinase activation. Moreover, Apocynin significantly ameliorated the loss of limb function (evaluated by motor recovery score). Thus, it is proposed that Apocynin may be useful in the treatment of inflammation associated with spinal cord trauma.
| Original language | English |
|---|---|
| Pages (from-to) | 221-236 |
| Number of pages | 16 |
| Journal | Free Radical Research |
| Volume | 45 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2011 |
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Keywords
- Apocynin
- Apoptosis
- Inflammation
- Oxidative stress
- SCI
ASJC Scopus subject areas
- Biochemistry
Cite this
Effect of Apocynin, an inhibitor of NADPH oxidase, in the inflammatory process induced by an experimental model of spinal cord injury. / Impellizzeri, Daniela; Mazzon, Emanuela; Esposito, Emanuela; Paterniti, Irene; Bramanti, Placido; Cuzzocrea, Salvatore.
In: Free Radical Research, Vol. 45, No. 2, 02.2011, p. 221-236.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of Apocynin, an inhibitor of NADPH oxidase, in the inflammatory process induced by an experimental model of spinal cord injury
AU - Impellizzeri, Daniela
AU - Mazzon, Emanuela
AU - Esposito, Emanuela
AU - Paterniti, Irene
AU - Bramanti, Placido
AU - Cuzzocrea, Salvatore
PY - 2011/2
Y1 - 2011/2
N2 - NADPH-oxidase is an enzyme responsible for reactive oxygen species production, and inhibition of this enzyme represents an attractive therapeutic target for the treatment of many diseases. The aim of this study was to investigate the effects of Apocynin, NADPH-oxidase inhibitor, in the modulation of secondary injury in the spinal cord. The injury was induced by application of vascular clips to the dura via a four-level T5T8 laminectomy in mice. Treatment with Apocynin 1 and 6 h after the trauma significantly decreased (1) the degree of spinal cord inflammation and tissue injury, (2) neutrophil infiltration, (3) adhesion molecule expression, (4) nuclear transcription factorκB expression, (5) nitrotyrosine and poly-ADP-ribose formation, (6) pro-inflammatory cytokines production, (7) apoptosis and (8) mitogen-activated protein kinase activation. Moreover, Apocynin significantly ameliorated the loss of limb function (evaluated by motor recovery score). Thus, it is proposed that Apocynin may be useful in the treatment of inflammation associated with spinal cord trauma.
AB - NADPH-oxidase is an enzyme responsible for reactive oxygen species production, and inhibition of this enzyme represents an attractive therapeutic target for the treatment of many diseases. The aim of this study was to investigate the effects of Apocynin, NADPH-oxidase inhibitor, in the modulation of secondary injury in the spinal cord. The injury was induced by application of vascular clips to the dura via a four-level T5T8 laminectomy in mice. Treatment with Apocynin 1 and 6 h after the trauma significantly decreased (1) the degree of spinal cord inflammation and tissue injury, (2) neutrophil infiltration, (3) adhesion molecule expression, (4) nuclear transcription factorκB expression, (5) nitrotyrosine and poly-ADP-ribose formation, (6) pro-inflammatory cytokines production, (7) apoptosis and (8) mitogen-activated protein kinase activation. Moreover, Apocynin significantly ameliorated the loss of limb function (evaluated by motor recovery score). Thus, it is proposed that Apocynin may be useful in the treatment of inflammation associated with spinal cord trauma.
KW - Apocynin
KW - Apoptosis
KW - Inflammation
KW - Oxidative stress
KW - SCI
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UR - http://www.scopus.com/inward/citedby.url?scp=78651267346&partnerID=8YFLogxK
U2 - 10.3109/10715762.2010.526604
DO - 10.3109/10715762.2010.526604
M3 - Article
VL - 45
SP - 221
EP - 236
JO - Free Radical Research
JF - Free Radical Research
SN - 1071-5762
IS - 2
ER -