Effect of Apocynin, an inhibitor of NADPH oxidase, in the inflammatory process induced by an experimental model of spinal cord injury

Daniela Impellizzeri, Emanuela Mazzon, Emanuela Esposito, Irene Paterniti, Placido Bramanti, Salvatore Cuzzocrea

Research output: Contribution to journalArticle

Abstract

NADPH-oxidase is an enzyme responsible for reactive oxygen species production, and inhibition of this enzyme represents an attractive therapeutic target for the treatment of many diseases. The aim of this study was to investigate the effects of Apocynin, NADPH-oxidase inhibitor, in the modulation of secondary injury in the spinal cord. The injury was induced by application of vascular clips to the dura via a four-level T5T8 laminectomy in mice. Treatment with Apocynin 1 and 6 h after the trauma significantly decreased (1) the degree of spinal cord inflammation and tissue injury, (2) neutrophil infiltration, (3) adhesion molecule expression, (4) nuclear transcription factorκB expression, (5) nitrotyrosine and poly-ADP-ribose formation, (6) pro-inflammatory cytokines production, (7) apoptosis and (8) mitogen-activated protein kinase activation. Moreover, Apocynin significantly ameliorated the loss of limb function (evaluated by motor recovery score). Thus, it is proposed that Apocynin may be useful in the treatment of inflammation associated with spinal cord trauma.

Original languageEnglish
Pages (from-to)221-236
Number of pages16
JournalFree Radical Research
Volume45
Issue number2
DOIs
Publication statusPublished - Feb 2011

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Keywords

  • Apocynin
  • Apoptosis
  • Inflammation
  • Oxidative stress
  • SCI

ASJC Scopus subject areas

  • Biochemistry

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