Effect of Apocynin, an inhibitor of NADPH oxidase, in the inflammatory process induced by an experimental model of spinal cord injury

Daniela Impellizzeri, Emanuela Mazzon, Emanuela Esposito, Irene Paterniti, Placido Bramanti, Salvatore Cuzzocrea

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

NADPH-oxidase is an enzyme responsible for reactive oxygen species production, and inhibition of this enzyme represents an attractive therapeutic target for the treatment of many diseases. The aim of this study was to investigate the effects of Apocynin, NADPH-oxidase inhibitor, in the modulation of secondary injury in the spinal cord. The injury was induced by application of vascular clips to the dura via a four-level T5T8 laminectomy in mice. Treatment with Apocynin 1 and 6 h after the trauma significantly decreased (1) the degree of spinal cord inflammation and tissue injury, (2) neutrophil infiltration, (3) adhesion molecule expression, (4) nuclear transcription factorκB expression, (5) nitrotyrosine and poly-ADP-ribose formation, (6) pro-inflammatory cytokines production, (7) apoptosis and (8) mitogen-activated protein kinase activation. Moreover, Apocynin significantly ameliorated the loss of limb function (evaluated by motor recovery score). Thus, it is proposed that Apocynin may be useful in the treatment of inflammation associated with spinal cord trauma.

Original languageEnglish
Pages (from-to)221-236
Number of pages16
JournalFree Radical Research
Volume45
Issue number2
DOIs
Publication statusPublished - Feb 2011

Fingerprint

NADPH Oxidase
Spinal Cord Injuries
Theoretical Models
Wounds and Injuries
Mitogen-Activated Protein Kinase 8
Myelitis
Poly Adenosine Diphosphate Ribose
Laminectomy
Neutrophil Infiltration
Enzymes
Transcription
Infiltration
Surgical Instruments
Blood Vessels
Reactive Oxygen Species
Adhesion
Extremities
Chemical activation
Modulation
Tissue

Keywords

  • Apocynin
  • Apoptosis
  • Inflammation
  • Oxidative stress
  • SCI

ASJC Scopus subject areas

  • Biochemistry

Cite this

Effect of Apocynin, an inhibitor of NADPH oxidase, in the inflammatory process induced by an experimental model of spinal cord injury. / Impellizzeri, Daniela; Mazzon, Emanuela; Esposito, Emanuela; Paterniti, Irene; Bramanti, Placido; Cuzzocrea, Salvatore.

In: Free Radical Research, Vol. 45, No. 2, 02.2011, p. 221-236.

Research output: Contribution to journalArticle

Impellizzeri, Daniela ; Mazzon, Emanuela ; Esposito, Emanuela ; Paterniti, Irene ; Bramanti, Placido ; Cuzzocrea, Salvatore. / Effect of Apocynin, an inhibitor of NADPH oxidase, in the inflammatory process induced by an experimental model of spinal cord injury. In: Free Radical Research. 2011 ; Vol. 45, No. 2. pp. 221-236.
@article{afa62865dcc14c9cbd13eb79c0fe71f3,
title = "Effect of Apocynin, an inhibitor of NADPH oxidase, in the inflammatory process induced by an experimental model of spinal cord injury",
abstract = "NADPH-oxidase is an enzyme responsible for reactive oxygen species production, and inhibition of this enzyme represents an attractive therapeutic target for the treatment of many diseases. The aim of this study was to investigate the effects of Apocynin, NADPH-oxidase inhibitor, in the modulation of secondary injury in the spinal cord. The injury was induced by application of vascular clips to the dura via a four-level T5T8 laminectomy in mice. Treatment with Apocynin 1 and 6 h after the trauma significantly decreased (1) the degree of spinal cord inflammation and tissue injury, (2) neutrophil infiltration, (3) adhesion molecule expression, (4) nuclear transcription factorκB expression, (5) nitrotyrosine and poly-ADP-ribose formation, (6) pro-inflammatory cytokines production, (7) apoptosis and (8) mitogen-activated protein kinase activation. Moreover, Apocynin significantly ameliorated the loss of limb function (evaluated by motor recovery score). Thus, it is proposed that Apocynin may be useful in the treatment of inflammation associated with spinal cord trauma.",
keywords = "Apocynin, Apoptosis, Inflammation, Oxidative stress, SCI",
author = "Daniela Impellizzeri and Emanuela Mazzon and Emanuela Esposito and Irene Paterniti and Placido Bramanti and Salvatore Cuzzocrea",
year = "2011",
month = "2",
doi = "10.3109/10715762.2010.526604",
language = "English",
volume = "45",
pages = "221--236",
journal = "Free Radical Research",
issn = "1071-5762",
publisher = "Informa Healthcare",
number = "2",

}

TY - JOUR

T1 - Effect of Apocynin, an inhibitor of NADPH oxidase, in the inflammatory process induced by an experimental model of spinal cord injury

AU - Impellizzeri, Daniela

AU - Mazzon, Emanuela

AU - Esposito, Emanuela

AU - Paterniti, Irene

AU - Bramanti, Placido

AU - Cuzzocrea, Salvatore

PY - 2011/2

Y1 - 2011/2

N2 - NADPH-oxidase is an enzyme responsible for reactive oxygen species production, and inhibition of this enzyme represents an attractive therapeutic target for the treatment of many diseases. The aim of this study was to investigate the effects of Apocynin, NADPH-oxidase inhibitor, in the modulation of secondary injury in the spinal cord. The injury was induced by application of vascular clips to the dura via a four-level T5T8 laminectomy in mice. Treatment with Apocynin 1 and 6 h after the trauma significantly decreased (1) the degree of spinal cord inflammation and tissue injury, (2) neutrophil infiltration, (3) adhesion molecule expression, (4) nuclear transcription factorκB expression, (5) nitrotyrosine and poly-ADP-ribose formation, (6) pro-inflammatory cytokines production, (7) apoptosis and (8) mitogen-activated protein kinase activation. Moreover, Apocynin significantly ameliorated the loss of limb function (evaluated by motor recovery score). Thus, it is proposed that Apocynin may be useful in the treatment of inflammation associated with spinal cord trauma.

AB - NADPH-oxidase is an enzyme responsible for reactive oxygen species production, and inhibition of this enzyme represents an attractive therapeutic target for the treatment of many diseases. The aim of this study was to investigate the effects of Apocynin, NADPH-oxidase inhibitor, in the modulation of secondary injury in the spinal cord. The injury was induced by application of vascular clips to the dura via a four-level T5T8 laminectomy in mice. Treatment with Apocynin 1 and 6 h after the trauma significantly decreased (1) the degree of spinal cord inflammation and tissue injury, (2) neutrophil infiltration, (3) adhesion molecule expression, (4) nuclear transcription factorκB expression, (5) nitrotyrosine and poly-ADP-ribose formation, (6) pro-inflammatory cytokines production, (7) apoptosis and (8) mitogen-activated protein kinase activation. Moreover, Apocynin significantly ameliorated the loss of limb function (evaluated by motor recovery score). Thus, it is proposed that Apocynin may be useful in the treatment of inflammation associated with spinal cord trauma.

KW - Apocynin

KW - Apoptosis

KW - Inflammation

KW - Oxidative stress

KW - SCI

UR - http://www.scopus.com/inward/record.url?scp=78651267346&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78651267346&partnerID=8YFLogxK

U2 - 10.3109/10715762.2010.526604

DO - 10.3109/10715762.2010.526604

M3 - Article

VL - 45

SP - 221

EP - 236

JO - Free Radical Research

JF - Free Radical Research

SN - 1071-5762

IS - 2

ER -