Two different human malignant melanomas from explanted tissue (MC2 and MC3) were treated with a sub-toxic concentration of azelaic acid (AA, 10 mM). Viability, growth rate, ultrastructural morphology and karyotype were evaluated at 15, 30, 60 and 90 days of treatment and 10 days after restitution of normal complete medium. A time-dependent activity of AA on cell proliferation and viability was observed during the treatment. After replacement with normal medium MC3 cells resumed a high growth rate, while MC2 cells maintained a lower proliferation rate. The ultrastructural observations showed an increased number of damaged cells during the treatment and, in the residual cells at 90 days, a normal morphology of mitochondria and a low level of melanogenesis. The cytogenetic analyses revealed, in the residual cells, the disappearance in MC2 cells of the two chromosomal markers previously present (M1 T(1:4) (q21:q21), M2 7q) and in MC3 cells, a significant (p <0.001) reduction of markers involving chromosome 1. The results show that long term treatment with AA has a selective toxic effect on undifferentiated cells with a high growth rate and bearing chromosomal aberrations.
|Number of pages||6|
|Journal||Cancer Journal (United States)|
|Publication status||Published - 1991|
ASJC Scopus subject areas
- Cancer Research