Effect of bacterial purified antigenic fractions on natural defence mechanisms

Walter Luini, Silvano Sozzani, Lino Braceschi, Annunciata Vecchi, Federico Spreafico

Research output: Contribution to journalArticlepeer-review

Abstract

The in vitro of bacterial purified antigenic fractions to interfere with the immune system has been investigated on human mononuclear cells from peripheral blood. Exposure of purified monocytes to the drug at concentrations from 1 to 1000 μg/ml, for different periods from 0 to 18 h, significant cell-mediated cytotoxicity against TU5 target cells. Moreover, monocytes exposed for 1 to 18 h to drug concentrations from 0.1 to 1000 μ/ml released significant amounts of tumor necrosis factor α in a dose-dependent manner in the culture supernatants. The drug was also tested on natural killer (NK) cell activity; mononuclear cells exposed to antigenic fractions for different periods showed a significant increase of NK cytotoxic activity against K562 target cells after 3 and 6, but not 0 and 18 h. Active concentrations were from 1 to 100 μm/ml, higher and lower doses being ineffective. Bacterial purified antigenic fractions thus have some ability to interfere in vitro with mechanisms of cytolysis mediated by cells and soluble factors.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalImmunopharmacology
Volume18
Issue number2
DOIs
Publication statusPublished - 1989

Keywords

  • Bacterial purified antigenic fraction
  • Natural defence
  • Natural killer activity
  • Tumor necrosis factor alpha (TNF α)

ASJC Scopus subject areas

  • Pharmacology

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