Effect of bezafibrate and clofibrate on the heme-iron geometry of ferrous nitrosylated heme-human serum albumin: An EPR study

Marco Mattu, Alessandro Vannini, Massimo Coletta, Mauro Fasano, Paolo Ascenzi

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of bezafibrate (BZF) and clofibrate (CF), two therapeutic drugs displaying anticoagulant and antihyperlipoproteinemic activities, on the EPR-spectroscopic properties of ferrous nitrosylated heme-human serum albumin (HSA-heme-NO) has been investigated. In the absence of BZF and CF, HSA-heme-NO is a five-coordinate heme-iron system, characterised by an X-band EPR spectrum with a three-line splitting in the high magnetic field region. Addition of BZF and CF to HSA-heme-NO induced the transition towards a six-coordinate heme-iron species characterised by an X-band EPR spectrum with an axial geometry. These data indicate that HSA-heme-NO is a five-coordinate heme-iron system, BZF and CF acting as allosteric effectors, and show that the primary heme binding site and the CF cleft of HSA are conformationally-linked, regardless of their different location.

Original languageEnglish
Pages (from-to)293-296
Number of pages4
JournalJournal of Inorganic Biochemistry
Volume84
Issue number3-4
Publication statusPublished - 2001

Keywords

  • Bezafibrate
  • Clofibrate
  • EPR spectroscopic properties
  • Ferrous nitrosylated heme-human serum albumin
  • Heme-iron geometry

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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