Abstract
The effect of bezafibrate (BZF) and clofibrate (CF), two therapeutic drugs displaying anticoagulant and antihyperlipoproteinemic activities, on the EPR-spectroscopic properties of ferrous nitrosylated heme-human serum albumin (HSA-heme-NO) has been investigated. In the absence of BZF and CF, HSA-heme-NO is a five-coordinate heme-iron system, characterised by an X-band EPR spectrum with a three-line splitting in the high magnetic field region. Addition of BZF and CF to HSA-heme-NO induced the transition towards a six-coordinate heme-iron species characterised by an X-band EPR spectrum with an axial geometry. These data indicate that HSA-heme-NO is a five-coordinate heme-iron system, BZF and CF acting as allosteric effectors, and show that the primary heme binding site and the CF cleft of HSA are conformationally-linked, regardless of their different location.
Original language | English |
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Pages (from-to) | 293-296 |
Number of pages | 4 |
Journal | Journal of Inorganic Biochemistry |
Volume | 84 |
Issue number | 3-4 |
Publication status | Published - 2001 |
Keywords
- Bezafibrate
- Clofibrate
- EPR spectroscopic properties
- Ferrous nitrosylated heme-human serum albumin
- Heme-iron geometry
ASJC Scopus subject areas
- Biochemistry
- Inorganic Chemistry