Effect of biotin on glucokinase activity, mRNA expression and insulin release in cultured beta cells

P. Borboni, R. Magnaterra, R. A. Rabini, R. Staffolani, O. Porzio, G. Sesti, A. Fusco, L. Mazzanti, R. Lauro, L. N J L Marlier

Research output: Contribution to journalArticlepeer-review

Abstract

Biotin is known to influence hepatic glucokinase (GK) expression both at a transcriptional and at a translational level. The aim of the present paper was to investigate the effect of biotin on pancreatic GK. For this purpose, RIN1046-38 cells were cultured in the presence of different biotin concentrations for different times; thereafter, GK mRNA expression, GK activity and insulin release were studied. Results demonstrated that biotin has a biphasic effect on GK mRNA expression, being stimulatory after short-term treatment and inhibitory after longterm treatment. GK activity was increased after long-term treatment. Insulin release was not affected by biotin treatment. These data suggest that biotin may influence glucose metabolism also by acting directly at the level of beta-cells.

Original languageEnglish
Pages (from-to)154-158
Number of pages5
JournalActa Diabetologica
Volume33
Issue number2
DOIs
Publication statusPublished - 1996

Keywords

  • Biotin
  • Glucokinase
  • Pancreatic islets

ASJC Scopus subject areas

  • Endocrinology
  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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