TY - JOUR
T1 - Effect of broccoli intake on markers related to oxidative stress and cancer risk in healthy smokers and nonsmokers
AU - Riso, Patrizia
AU - Martini, Daniela
AU - Visioli, Francesco
AU - Martinetti, Antonia
AU - Porrini, Marisa
PY - 2009/3
Y1 - 2009/3
N2 - Cruciferous vegetables (CVs) have been widely studied for their anticarcinogenic properties. The aim of the present study was to evaluate the protective effect of broccoli intake in smokers and nonsmokers. Twenty young healthy males (10 smokers and 10 nonsmokers) were randomized in a cross-over design and received a portion of broccoli (200 g) or maintained a controlled diet for 10 days each. The two periods were separated by a wash out period (20 days). Blood samples were collected at 0, 10, 30, and 40 days and used for the evaluation of DNA damage, insulin-like growth factor-I (IGF-I) and histone deacetylase (HDAC). Ex vivo protection from H2O2-induced DNA damage and endogenous DNA damage were evaluated in lymphocytes by means of the comet assay. Strand breaks decreased significantly after the broccoli diet in smokers as well as in nonsmokers (-22.2%; P <0.0001), whereas oxidized purines decreased significantly only in smokers (-51.0%; P <0.0001). Broccoli intake did not modify HDAC activity and IGF-I serum levels. Our results strengthen the importance of consuming CVs to increase cell protection against DNA damage. Future investigation, with different amount of broccoli and/or different time of exposure, is needed to understand the lack of effect on HDAC activity and IGF-I levels.
AB - Cruciferous vegetables (CVs) have been widely studied for their anticarcinogenic properties. The aim of the present study was to evaluate the protective effect of broccoli intake in smokers and nonsmokers. Twenty young healthy males (10 smokers and 10 nonsmokers) were randomized in a cross-over design and received a portion of broccoli (200 g) or maintained a controlled diet for 10 days each. The two periods were separated by a wash out period (20 days). Blood samples were collected at 0, 10, 30, and 40 days and used for the evaluation of DNA damage, insulin-like growth factor-I (IGF-I) and histone deacetylase (HDAC). Ex vivo protection from H2O2-induced DNA damage and endogenous DNA damage were evaluated in lymphocytes by means of the comet assay. Strand breaks decreased significantly after the broccoli diet in smokers as well as in nonsmokers (-22.2%; P <0.0001), whereas oxidized purines decreased significantly only in smokers (-51.0%; P <0.0001). Broccoli intake did not modify HDAC activity and IGF-I serum levels. Our results strengthen the importance of consuming CVs to increase cell protection against DNA damage. Future investigation, with different amount of broccoli and/or different time of exposure, is needed to understand the lack of effect on HDAC activity and IGF-I levels.
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U2 - 10.1080/01635580802425688
DO - 10.1080/01635580802425688
M3 - Article
C2 - 19235039
AN - SCOPUS:61649124922
VL - 61
SP - 232
EP - 237
JO - Nutrition and Cancer
JF - Nutrition and Cancer
SN - 0163-5581
IS - 2
ER -