Effect of capacitation on the endocannabinoid system of mouse sperm

Giuseppina Catanzaro, Natalia Battista, Gianna Rossi, Monia Di Tommaso, Mariangela Pucci, Valentina Pirazzi, Sandra Cecconi, Mauro Maccarrone

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The presence of the elements of the endocannabinoid system (ECS) in sperm isolated from several species (from invertebrates to mammals, humans included) has supported the "evolutionary theory" that proposes endocannabinoids as check points in reproductive events like capacitation. In this study, we characterized the ECS elements at the mRNA, protein and functional levels in mouse sperm before and after capacitation. We found that the latter process increases the endogenous levels of the two major endocannabinoids (anandamide and 2-arachidonoylglycerol), through a decreased degradation and increased biosynthesis, respectively. Additionally, we found that the binding activity of cannabinoid receptors was not affected by sperm capacitation, whereas that of vanilloid receptor was reduced. Overall, our data demonstrate that mouse sperm have a fully functional ECS, and that capacitation alters the endogenous tone of the major endocannabinoids through distinct mechanisms.

Original languageEnglish
Pages (from-to)88-92
Number of pages5
JournalMolecular and Cellular Endocrinology
Volume343
Issue number1-2
DOIs
Publication statusPublished - Aug 22 2011

Fingerprint

Endocannabinoids
Spermatozoa
Sperm Capacitation
TRPV Cation Channels
Cannabinoid Receptors
Mammals
Biosynthesis
Invertebrates
Degradation
Messenger RNA
Proteins

Keywords

  • 2-Arachidonoylglycerol
  • Anandamide
  • Capacitation
  • Mouse sperm

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Cite this

Effect of capacitation on the endocannabinoid system of mouse sperm. / Catanzaro, Giuseppina; Battista, Natalia; Rossi, Gianna; Di Tommaso, Monia; Pucci, Mariangela; Pirazzi, Valentina; Cecconi, Sandra; Maccarrone, Mauro.

In: Molecular and Cellular Endocrinology, Vol. 343, No. 1-2, 22.08.2011, p. 88-92.

Research output: Contribution to journalArticle

Catanzaro, G, Battista, N, Rossi, G, Di Tommaso, M, Pucci, M, Pirazzi, V, Cecconi, S & Maccarrone, M 2011, 'Effect of capacitation on the endocannabinoid system of mouse sperm', Molecular and Cellular Endocrinology, vol. 343, no. 1-2, pp. 88-92. https://doi.org/10.1016/j.mce.2011.01.022
Catanzaro G, Battista N, Rossi G, Di Tommaso M, Pucci M, Pirazzi V et al. Effect of capacitation on the endocannabinoid system of mouse sperm. Molecular and Cellular Endocrinology. 2011 Aug 22;343(1-2):88-92. https://doi.org/10.1016/j.mce.2011.01.022
Catanzaro, Giuseppina ; Battista, Natalia ; Rossi, Gianna ; Di Tommaso, Monia ; Pucci, Mariangela ; Pirazzi, Valentina ; Cecconi, Sandra ; Maccarrone, Mauro. / Effect of capacitation on the endocannabinoid system of mouse sperm. In: Molecular and Cellular Endocrinology. 2011 ; Vol. 343, No. 1-2. pp. 88-92.
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