Abstract
Purpose To verify the effect of vasostatin-1 (VS-1), an anti-angiogenic fragment of chromogranin A, in the prevention of choroidal neovascularization (CNV) in an established mouse model of laser-induced ocular neovascularization. Methods Bruch's membrane, the innermost layer of the choroid, was broken by laser photocoagulation in C57/Bl6 mice, to induce CNV. Mice were then treated daily for 14 days by intraperitoneal injection of VS-1 or vehicle (6 mice/group). CNV and vascular leakage were measured at three time-points (day 0, 7 and 14) in vivo by spectral domain optical coherence tomography (OCT) and fluorescein angiography (FA). Ex vivo analysis of CNV was also performed at day 14 by confocal microscopy analysis of dextran-perfused choroidal flat-mounts. Results In vivo analyses showed that VS-1 significantly reduced CNV at day 14 (p = 0.03) and vascular leakage at day 7 (p = 0.01) and 14 (p = 0.04). Ex vivo confocal microscopy analysis of CNV performed on dextran-perfused choroidal flat-mounts at day 14 confirmed the protective activity of VS-1 (p = 0.01). A significant correlation between the results of in vivo and ex vivo analyses of CNV was also observed (p = 0.001, R2 = 0.81). Conclusion The results indicate that VS-1 can prevent CNV and vascular leakage in a mouse model of ocular neovascularization, suggesting that this polypeptide might have therapeutic activity in human ocular diseases that are complicated by neovascularization or excessive vascular permeability.
| Original language | English |
|---|---|
| Pages (from-to) | e218-e222 |
| Journal | Acta Ophthalmologica |
| Volume | 93 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - May 1 2015 |
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Keywords
- mouse model
- ocular neovascularization
- ophthalmic coherence tomography
- vasostatin-1
ASJC Scopus subject areas
- Ophthalmology
- Medicine(all)
Cite this
Effect of chromogranin A-derived vasostatin-1 on laser-induced choroidal neovascularization in the mouse. / Maestroni, Silvia; Maestroni, Anna; Ceglia, Simona; Tremolada, Gemma; Mancino, Monica; Sacchi, Angelina; Lattanzio, Rosangela; Zucchiatti, Ilaria; Corti, Angelo; Bandello, Francesco; Zerbini, Gianpaolo.
In: Acta Ophthalmologica, Vol. 93, No. 3, 01.05.2015, p. e218-e222.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of chromogranin A-derived vasostatin-1 on laser-induced choroidal neovascularization in the mouse
AU - Maestroni, Silvia
AU - Maestroni, Anna
AU - Ceglia, Simona
AU - Tremolada, Gemma
AU - Mancino, Monica
AU - Sacchi, Angelina
AU - Lattanzio, Rosangela
AU - Zucchiatti, Ilaria
AU - Corti, Angelo
AU - Bandello, Francesco
AU - Zerbini, Gianpaolo
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Purpose To verify the effect of vasostatin-1 (VS-1), an anti-angiogenic fragment of chromogranin A, in the prevention of choroidal neovascularization (CNV) in an established mouse model of laser-induced ocular neovascularization. Methods Bruch's membrane, the innermost layer of the choroid, was broken by laser photocoagulation in C57/Bl6 mice, to induce CNV. Mice were then treated daily for 14 days by intraperitoneal injection of VS-1 or vehicle (6 mice/group). CNV and vascular leakage were measured at three time-points (day 0, 7 and 14) in vivo by spectral domain optical coherence tomography (OCT) and fluorescein angiography (FA). Ex vivo analysis of CNV was also performed at day 14 by confocal microscopy analysis of dextran-perfused choroidal flat-mounts. Results In vivo analyses showed that VS-1 significantly reduced CNV at day 14 (p = 0.03) and vascular leakage at day 7 (p = 0.01) and 14 (p = 0.04). Ex vivo confocal microscopy analysis of CNV performed on dextran-perfused choroidal flat-mounts at day 14 confirmed the protective activity of VS-1 (p = 0.01). A significant correlation between the results of in vivo and ex vivo analyses of CNV was also observed (p = 0.001, R2 = 0.81). Conclusion The results indicate that VS-1 can prevent CNV and vascular leakage in a mouse model of ocular neovascularization, suggesting that this polypeptide might have therapeutic activity in human ocular diseases that are complicated by neovascularization or excessive vascular permeability.
AB - Purpose To verify the effect of vasostatin-1 (VS-1), an anti-angiogenic fragment of chromogranin A, in the prevention of choroidal neovascularization (CNV) in an established mouse model of laser-induced ocular neovascularization. Methods Bruch's membrane, the innermost layer of the choroid, was broken by laser photocoagulation in C57/Bl6 mice, to induce CNV. Mice were then treated daily for 14 days by intraperitoneal injection of VS-1 or vehicle (6 mice/group). CNV and vascular leakage were measured at three time-points (day 0, 7 and 14) in vivo by spectral domain optical coherence tomography (OCT) and fluorescein angiography (FA). Ex vivo analysis of CNV was also performed at day 14 by confocal microscopy analysis of dextran-perfused choroidal flat-mounts. Results In vivo analyses showed that VS-1 significantly reduced CNV at day 14 (p = 0.03) and vascular leakage at day 7 (p = 0.01) and 14 (p = 0.04). Ex vivo confocal microscopy analysis of CNV performed on dextran-perfused choroidal flat-mounts at day 14 confirmed the protective activity of VS-1 (p = 0.01). A significant correlation between the results of in vivo and ex vivo analyses of CNV was also observed (p = 0.001, R2 = 0.81). Conclusion The results indicate that VS-1 can prevent CNV and vascular leakage in a mouse model of ocular neovascularization, suggesting that this polypeptide might have therapeutic activity in human ocular diseases that are complicated by neovascularization or excessive vascular permeability.
KW - mouse model
KW - ocular neovascularization
KW - ophthalmic coherence tomography
KW - vasostatin-1
UR - http://www.scopus.com/inward/record.url?scp=84927694956&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84927694956&partnerID=8YFLogxK
U2 - 10.1111/aos.12557
DO - 10.1111/aos.12557
M3 - Article
VL - 93
SP - e218-e222
JO - Acta Ophthalmologica
JF - Acta Ophthalmologica
SN - 1755-375X
IS - 3
ER -