Effect of clotting factors concentrates on lymphocyte and neutrophil function in vitro: Relevance of soluble HLA class I, soluble Fas ligand and transforming growth factor β1

Massimo Ghio, Paola Contini, Luciano Ottonello, Nicoletta Arduino, Alessandro Gringeri, Francesco Indiveri, Franco Dallegri, Francesco Puppo

Research output: Contribution to journalArticle

Abstract

Immunological abnormalities have been reported in haemophiliacs. Although infections with HIV, hepatitis and other viruses may contribute to these abnormalities, immune defects are detectable also in HIV seronegative haemophiliacs. It is likely that chronic exposure to extraneous proteins in clotting factor concentrates (CFCs) may play a role in immunomodulation, but the underlying mechanisms remain unclear. The results of the present paper show that: a) soluble HLA class I (sHLA-1), soluble Fas-ligand (sFas-L) and transforming growth factor beta 1 (TGF-β1) are detectable in plasma derived but not in recombinant CFCs; b) the level of sHLA-I and sFas-L is proportional to the grade of CFCs purity whereas TGF-β1 showed very variable levels; c) soluble molecules detected in CFCs exert immunomodulatory effects in vitro like apoptosis induction in Jurkat cells and inhibition of mixed lymphocyte reaction response, antigen-specific lymphocyte cytotoxic activity and neutrophil chemotaxis.

Original languageEnglish
Pages (from-to)365-373
Number of pages9
JournalThrombosis and Haemostasis
Volume89
Issue number2
Publication statusPublished - Feb 1 2003

Keywords

  • Clotting factors concentrates
  • Soluble Fas ligand
  • Soluble HLA class I
  • Transforming growth factor β1

ASJC Scopus subject areas

  • Hematology

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