Abstract
Immunological abnormalities have been reported in haemophiliacs. Although infections with HIV, hepatitis and other viruses may contribute to these abnormalities, immune defects are detectable also in HIV seronegative haemophiliacs. It is likely that chronic exposure to extraneous proteins in clotting factor concentrates (CFCs) may play a role in immunomodulation, but the underlying mechanisms remain unclear. The results of the present paper show that: a) soluble HLA class I (sHLA-1), soluble Fas-ligand (sFas-L) and transforming growth factor beta 1 (TGF-β1) are detectable in plasma derived but not in recombinant CFCs; b) the level of sHLA-I and sFas-L is proportional to the grade of CFCs purity whereas TGF-β1 showed very variable levels; c) soluble molecules detected in CFCs exert immunomodulatory effects in vitro like apoptosis induction in Jurkat cells and inhibition of mixed lymphocyte reaction response, antigen-specific lymphocyte cytotoxic activity and neutrophil chemotaxis.
| Original language | English |
|---|---|
| Pages (from-to) | 365-373 |
| Number of pages | 9 |
| Journal | Thrombosis and Haemostasis |
| Volume | 89 |
| Issue number | 2 |
| Publication status | Published - Feb 1 2003 |
Fingerprint
Keywords
- Clotting factors concentrates
- Soluble Fas ligand
- Soluble HLA class I
- Transforming growth factor β1
ASJC Scopus subject areas
- Hematology
Cite this
Effect of clotting factors concentrates on lymphocyte and neutrophil function in vitro : Relevance of soluble HLA class I, soluble Fas ligand and transforming growth factor β1. / Ghio, Massimo; Contini, Paola; Ottonello, Luciano; Arduino, Nicoletta; Gringeri, Alessandro; Indiveri, Francesco; Dallegri, Franco; Puppo, Francesco.
In: Thrombosis and Haemostasis, Vol. 89, No. 2, 01.02.2003, p. 365-373.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of clotting factors concentrates on lymphocyte and neutrophil function in vitro
T2 - Relevance of soluble HLA class I, soluble Fas ligand and transforming growth factor β1
AU - Ghio, Massimo
AU - Contini, Paola
AU - Ottonello, Luciano
AU - Arduino, Nicoletta
AU - Gringeri, Alessandro
AU - Indiveri, Francesco
AU - Dallegri, Franco
AU - Puppo, Francesco
PY - 2003/2/1
Y1 - 2003/2/1
N2 - Immunological abnormalities have been reported in haemophiliacs. Although infections with HIV, hepatitis and other viruses may contribute to these abnormalities, immune defects are detectable also in HIV seronegative haemophiliacs. It is likely that chronic exposure to extraneous proteins in clotting factor concentrates (CFCs) may play a role in immunomodulation, but the underlying mechanisms remain unclear. The results of the present paper show that: a) soluble HLA class I (sHLA-1), soluble Fas-ligand (sFas-L) and transforming growth factor beta 1 (TGF-β1) are detectable in plasma derived but not in recombinant CFCs; b) the level of sHLA-I and sFas-L is proportional to the grade of CFCs purity whereas TGF-β1 showed very variable levels; c) soluble molecules detected in CFCs exert immunomodulatory effects in vitro like apoptosis induction in Jurkat cells and inhibition of mixed lymphocyte reaction response, antigen-specific lymphocyte cytotoxic activity and neutrophil chemotaxis.
AB - Immunological abnormalities have been reported in haemophiliacs. Although infections with HIV, hepatitis and other viruses may contribute to these abnormalities, immune defects are detectable also in HIV seronegative haemophiliacs. It is likely that chronic exposure to extraneous proteins in clotting factor concentrates (CFCs) may play a role in immunomodulation, but the underlying mechanisms remain unclear. The results of the present paper show that: a) soluble HLA class I (sHLA-1), soluble Fas-ligand (sFas-L) and transforming growth factor beta 1 (TGF-β1) are detectable in plasma derived but not in recombinant CFCs; b) the level of sHLA-I and sFas-L is proportional to the grade of CFCs purity whereas TGF-β1 showed very variable levels; c) soluble molecules detected in CFCs exert immunomodulatory effects in vitro like apoptosis induction in Jurkat cells and inhibition of mixed lymphocyte reaction response, antigen-specific lymphocyte cytotoxic activity and neutrophil chemotaxis.
KW - Clotting factors concentrates
KW - Soluble Fas ligand
KW - Soluble HLA class I
KW - Transforming growth factor β1
UR - http://www.scopus.com/inward/record.url?scp=0037326753&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037326753&partnerID=8YFLogxK
M3 - Article
C2 - 12574818
AN - SCOPUS:0037326753
VL - 89
SP - 365
EP - 373
JO - Thrombosis and Haemostasis
JF - Thrombosis and Haemostasis
SN - 0340-6245
IS - 2
ER -