The influence of complement activation through the alternative pathway on PMN adherence to nylon fibers was investigated using either heparinized plasma or serum from blood donors. Reduced granulocyte binding was observed in 21 out of 34 yeast-treated samples and in 4 out of 18 inulin-activated samples and was more evident with serum than with plasma. The presence of the inhibitory factor in the sera was related to the development of reactor activity (C56) which was induced in the sera by yeast cells through the activation of the alternative pathway. The inhibitor was found to be heat stable, of high molecular weight, and was neutralized by the antiserum against human C5. Separate incubation of the inhibitory factor with either nylon fibers or PMN showed its binding to nylon fibers and the results suggest a competitive activity of the factor with PMN for nylon fibers.
ASJC Scopus subject areas
- Immunology and Allergy
- Pathology and Forensic Medicine