Effect of dicarboxylic acids on Harding-Passey and Cloudman S91 melanoma cells in tissue culture

E. J. Robins, A. S. Breathnach, B. J. Ward, Y. P. Bhasin, L. Ethridge, M. Nazzaro-Porro, S. Passi, M. Picardo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Clinically, dicarboxylic acids have a cytotoxic effect on the abnormally hyperactive and malignant epidermal melanocyte, and diacids from C8 to C13 have been shown to inhibit mitochondrial oxidoreductases. Here, their effect on the growth kinetics and ultrastructure of murine melanoma cells in culture is examined. Cultures of Harding-Passey and Cloudman S91 melanoma cells were exposed to single doses of the disodium salts of C12, C9, and C6 (which does not significantly inhibit mitochondrial enzymes) dicarboxylic acids at concentrations of 10-3 M to 10-1 M. With C12 and C9, viability and cell proliferation over 3 days were significantly affected by concentrations greater than 102 M. With exposure to C6 at 10-1 M and to medium to which NaCl was added to produce equal osmolarity, the effect was much less. Electron microscopy of cell exposed to C9 at 10-1 M for 1 h and 6 h revealed massive swelling of mitochondria with destruction of cristae, but plasma and nuclear membranes and membranes of endoplasmic reticulum were intact. Similar damage was not seen with C6 at 10-1 M nor with equiosmolar NaCl. The results confirm (1) the cytotoxicity of dicarboxylic acids for malignant melanocytes, and (2) that the mitochondrion is a prime target for their action.

Original languageEnglish
Pages (from-to)216-221
Number of pages6
JournalJournal of Investigative Dermatology
Volume85
Issue number3
Publication statusPublished - 1985

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Tissue culture
Dicarboxylic Acids
Experimental Melanomas
Mitochondria
Melanocytes
Membranes
Growth kinetics
Nuclear Envelope
Cell proliferation
Cytotoxicity
Endoplasmic Reticulum
Osmolar Concentration
Electron microscopy
Swelling
Melanoma
Electron Microscopy
Oxidoreductases
Cell Culture Techniques
Salts
Cell Proliferation

ASJC Scopus subject areas

  • Dermatology

Cite this

Robins, E. J., Breathnach, A. S., Ward, B. J., Bhasin, Y. P., Ethridge, L., Nazzaro-Porro, M., ... Picardo, M. (1985). Effect of dicarboxylic acids on Harding-Passey and Cloudman S91 melanoma cells in tissue culture. Journal of Investigative Dermatology, 85(3), 216-221.

Effect of dicarboxylic acids on Harding-Passey and Cloudman S91 melanoma cells in tissue culture. / Robins, E. J.; Breathnach, A. S.; Ward, B. J.; Bhasin, Y. P.; Ethridge, L.; Nazzaro-Porro, M.; Passi, S.; Picardo, M.

In: Journal of Investigative Dermatology, Vol. 85, No. 3, 1985, p. 216-221.

Research output: Contribution to journalArticle

Robins, EJ, Breathnach, AS, Ward, BJ, Bhasin, YP, Ethridge, L, Nazzaro-Porro, M, Passi, S & Picardo, M 1985, 'Effect of dicarboxylic acids on Harding-Passey and Cloudman S91 melanoma cells in tissue culture', Journal of Investigative Dermatology, vol. 85, no. 3, pp. 216-221.
Robins EJ, Breathnach AS, Ward BJ, Bhasin YP, Ethridge L, Nazzaro-Porro M et al. Effect of dicarboxylic acids on Harding-Passey and Cloudman S91 melanoma cells in tissue culture. Journal of Investigative Dermatology. 1985;85(3):216-221.
Robins, E. J. ; Breathnach, A. S. ; Ward, B. J. ; Bhasin, Y. P. ; Ethridge, L. ; Nazzaro-Porro, M. ; Passi, S. ; Picardo, M. / Effect of dicarboxylic acids on Harding-Passey and Cloudman S91 melanoma cells in tissue culture. In: Journal of Investigative Dermatology. 1985 ; Vol. 85, No. 3. pp. 216-221.
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