Effect of dicarboxylic (C6 and C9) acids on a human squamous carcinoma cell line in cultures

H. C. Patzold; A. S. Breathnach; E. J. Robins; M. E. Daridan; Y. P. Bhasin; L. B. Ethridge; M. Nazzaro-Porro; S. Passi; M. Picardo

Effect of dicarboxylic (C6 and C9) acids on a human squamous carcinoma cell line in cultures

H. C. Patzold, A. S. Breathnach, E. J. Robins, M. E. Daridan, Y. P. Bhasin, L. B. Ethridge, M. Nazzaro-Porro, S. Passi, M. Picardo

Istituto S. Maria e S. Gallicano

Research output: Contribution to journal › Article › peer-review

Abstract

In tissue culture, azelaic acid (C₉) has been shown to have an anti-proliferative and cytotoxic effect on human and murine malignant melanocytes, with inhibition of mitochondrial oxido-reductase enzymes and DNA synthesis, and damage to mitochondria. Recent reports of effects on differentiation of normal keratocytes have led to the present study of its effects on a squamous carcinoma cell line. Cells were exposed to single doses of disodium salts of azelaic (C₉2Na) and adipic (C₆2Na) acids at concentrations of 10^-2 M and 5 x 10^-2 M for 48 hrs. Only C₉2Na at 5 x 10^-2 M for 4 hrs., and longer, significantly affected proliferation, and the cells exhibited massive swelling of mitochondria with loss of cristae. The results further confirm the probable value of azelaic acid as a general anti-tumoral agent rather than a specifically melanocytotoxic one. They could justify clinical studies on the effect of topical azelaic acid therapy on squamous cell carcinoma in vivo.

Original language	English
Pages (from-to)	167-171
Number of pages	5
Journal	Histology and Histopathology
Volume	4
Issue number	2
Publication status	Published - 1989

ASJC Scopus subject areas

Cell Biology
Anatomy
Histology
Pathology and Forensic Medicine

Cite this

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title = "Effect of dicarboxylic (C6 and C9) acids on a human squamous carcinoma cell line in cultures",

abstract = "In tissue culture, azelaic acid (C9) has been shown to have an anti-proliferative and cytotoxic effect on human and murine malignant melanocytes, with inhibition of mitochondrial oxido-reductase enzymes and DNA synthesis, and damage to mitochondria. Recent reports of effects on differentiation of normal keratocytes have led to the present study of its effects on a squamous carcinoma cell line. Cells were exposed to single doses of disodium salts of azelaic (C92Na) and adipic (C62Na) acids at concentrations of 10-2 M and 5 x 10-2 M for 48 hrs. Only C92Na at 5 x 10-2 M for 4 hrs., and longer, significantly affected proliferation, and the cells exhibited massive swelling of mitochondria with loss of cristae. The results further confirm the probable value of azelaic acid as a general anti-tumoral agent rather than a specifically melanocytotoxic one. They could justify clinical studies on the effect of topical azelaic acid therapy on squamous cell carcinoma in vivo.",

author = "Patzold, {H. C.} and Breathnach, {A. S.} and Robins, {E. J.} and Daridan, {M. E.} and Bhasin, {Y. P.} and Ethridge, {L. B.} and M. Nazzaro-Porro and S. Passi and M. Picardo",

year = "1989",

language = "English",

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pages = "167--171",

journal = "Histology and Histopathology",

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TY - JOUR

T1 - Effect of dicarboxylic (C6 and C9) acids on a human squamous carcinoma cell line in cultures

AU - Patzold, H. C.

AU - Breathnach, A. S.

AU - Robins, E. J.

AU - Daridan, M. E.

AU - Bhasin, Y. P.

AU - Ethridge, L. B.

AU - Nazzaro-Porro, M.

AU - Passi, S.

AU - Picardo, M.

PY - 1989

Y1 - 1989

N2 - In tissue culture, azelaic acid (C9) has been shown to have an anti-proliferative and cytotoxic effect on human and murine malignant melanocytes, with inhibition of mitochondrial oxido-reductase enzymes and DNA synthesis, and damage to mitochondria. Recent reports of effects on differentiation of normal keratocytes have led to the present study of its effects on a squamous carcinoma cell line. Cells were exposed to single doses of disodium salts of azelaic (C92Na) and adipic (C62Na) acids at concentrations of 10-2 M and 5 x 10-2 M for 48 hrs. Only C92Na at 5 x 10-2 M for 4 hrs., and longer, significantly affected proliferation, and the cells exhibited massive swelling of mitochondria with loss of cristae. The results further confirm the probable value of azelaic acid as a general anti-tumoral agent rather than a specifically melanocytotoxic one. They could justify clinical studies on the effect of topical azelaic acid therapy on squamous cell carcinoma in vivo.

AB - In tissue culture, azelaic acid (C9) has been shown to have an anti-proliferative and cytotoxic effect on human and murine malignant melanocytes, with inhibition of mitochondrial oxido-reductase enzymes and DNA synthesis, and damage to mitochondria. Recent reports of effects on differentiation of normal keratocytes have led to the present study of its effects on a squamous carcinoma cell line. Cells were exposed to single doses of disodium salts of azelaic (C92Na) and adipic (C62Na) acids at concentrations of 10-2 M and 5 x 10-2 M for 48 hrs. Only C92Na at 5 x 10-2 M for 4 hrs., and longer, significantly affected proliferation, and the cells exhibited massive swelling of mitochondria with loss of cristae. The results further confirm the probable value of azelaic acid as a general anti-tumoral agent rather than a specifically melanocytotoxic one. They could justify clinical studies on the effect of topical azelaic acid therapy on squamous cell carcinoma in vivo.

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VL - 4

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JO - Histology and Histopathology

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SN - 0213-3911

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ER -

Effect of dicarboxylic (C6 and C9) acids on a human squamous carcinoma cell line in cultures

Abstract

ASJC Scopus subject areas

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