Effect of different platelet agonists on intracellular free Ca++ concentrations in human tumor cells: Possible role in tumor growth

A. Saporiti, A. Brocchieri, C. Porta, M. Moroni, G. Grignani

Research output: Contribution to journalArticlepeer-review

Abstract

Modulation of cytoplasmic Ca++ concentration is a mechanism common to signal transduction pathways regulating many cellular phenomena, including the interactions of tumors with the hemostatic system. We have investigated the pro-aggregating and pro coagulant activities of human tumor cell lines cultured in vitro and the ability of different platelet agonists to induce Ca++ transients in these cells. Cells of a malignant mesothelioma line activated platelets by a thrombin-dependent mechanism; on the contrary, HeLa cells, derived from a uterine cervical cancer, possessed ADP-dependent pro-aggregating activity, and DND-IA melanoma cells did not stimulate platelet aggregation. All cell lines showed a tissue-factor-like procoagulant property, more pronounced in mesothelioma cells. Furthermore, ADP was able to induce a transient increase in cytoplasmic Ca++ concentration in tumor cells from all lines; collagen showed this effect in mesothelioma cells and in HeLa cells, and thrombin was effective only in mesothelioma cells. PAF never induced Ca++ fluxes in any of the cell lines investigated. Finally, the calcium channel blocker verapamil inhibited agonist-induced Ca++ transients in tumor cells and in vitro tumor-cell growth. These data may help to identify new possible mechanisms of the 2-way interaction of tumors with the hemostatic system.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalInternational Journal of Cancer
Volume62
Issue number3
DOIs
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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