Effect of different platelet agonists on intracellular free Ca++ concentrations in human tumor cells: Possible role in tumor growth

A. Saporiti, A. Brocchieri, C. Porta, M. Moroni, G. Grignani

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Modulation of cytoplasmic Ca++ concentration is a mechanism common to signal transduction pathways regulating many cellular phenomena, including the interactions of tumors with the hemostatic system. We have investigated the pro-aggregating and pro coagulant activities of human tumor cell lines cultured in vitro and the ability of different platelet agonists to induce Ca++ transients in these cells. Cells of a malignant mesothelioma line activated platelets by a thrombin-dependent mechanism; on the contrary, HeLa cells, derived from a uterine cervical cancer, possessed ADP-dependent pro-aggregating activity, and DND-IA melanoma cells did not stimulate platelet aggregation. All cell lines showed a tissue-factor-like procoagulant property, more pronounced in mesothelioma cells. Furthermore, ADP was able to induce a transient increase in cytoplasmic Ca++ concentration in tumor cells from all lines; collagen showed this effect in mesothelioma cells and in HeLa cells, and thrombin was effective only in mesothelioma cells. PAF never induced Ca++ fluxes in any of the cell lines investigated. Finally, the calcium channel blocker verapamil inhibited agonist-induced Ca++ transients in tumor cells and in vitro tumor-cell growth. These data may help to identify new possible mechanisms of the 2-way interaction of tumors with the hemostatic system.

Original languageEnglish
Pages (from-to)291-296
Number of pages6
JournalInternational Journal of Cancer
Volume62
Issue number3
DOIs
Publication statusPublished - 1995

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Blood Platelets
Growth
Neoplasms
Mesothelioma
Hemostatics
Tumor Cell Line
HeLa Cells
Thrombin
Adenosine Diphosphate
Cell Line
Coagulants
Calcium Channel Blockers
Thromboplastin
Verapamil
Platelet Aggregation
Human Activities
Uterine Cervical Neoplasms
Melanoma
Signal Transduction
Collagen

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Effect of different platelet agonists on intracellular free Ca++ concentrations in human tumor cells : Possible role in tumor growth. / Saporiti, A.; Brocchieri, A.; Porta, C.; Moroni, M.; Grignani, G.

In: International Journal of Cancer, Vol. 62, No. 3, 1995, p. 291-296.

Research output: Contribution to journalArticle

Saporiti, A. ; Brocchieri, A. ; Porta, C. ; Moroni, M. ; Grignani, G. / Effect of different platelet agonists on intracellular free Ca++ concentrations in human tumor cells : Possible role in tumor growth. In: International Journal of Cancer. 1995 ; Vol. 62, No. 3. pp. 291-296.
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