Effect of electrospun fiber diameter and alignment on macrophage activation and secretion of proinflammatory cytokines and chemokines

Enrica Saino, Maria Letizia Focarete, Chiara Gualandi, Enzo Emanuele, Antonia I. Cornaglia, Marcello Imbriani, Livia Visai

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Macrophage activation can be modulated by biomaterial topography according to the biological scale (micrometric and nanometric range). In this study, we investigated the effect of fiber diameter and fiber alignment of electrospun poly(l-lactic) (PLLA) scaffolds on macrophage RAW 264.7 activation and secretion of proinflammatory cytokines and chemokines at 24 h and 7 days. Macrophages were cultured on four different types of fibrous PLLA scaffold (aligned microfibers, aligned nanofibers, random microfibers, and random nanofibers) and on PLLA film (used as a reference). Substrate topography was found to influence the immune response activated by macrophages, especially in the early inflammation stage. Secretion of proinflammatory molecules by macrophage cells was chiefly dependent on fiber diameter. In particular, nanofibrous PLLA scaffolds minimized the inflammatory response when compared with films and microfibrous scaffolds. The histological evaluation demonstrated a higher number of foreign body giant cells on the PLLA film than on the micro- and nanofibrous scaffolds. In summary, our results indicate that the diameter of electrospun PLLA fibers, rather than fiber alignment, plays a relevant role in influencing in vitro macrophage activation and secretion of proinflammatory molecules.

Original languageEnglish
Pages (from-to)1900-1911
Number of pages12
JournalBiomacromolecules
Volume12
Issue number5
DOIs
Publication statusPublished - May 9 2011

Fingerprint

Macrophages
Chemokines
Chemical activation
Cytokines
Scaffolds
Fibers
Nanofibers
Topography
Molecules
Biocompatible Materials
Scaffolds (biology)
Biomaterials
Milk
Substrates

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials

Cite this

Effect of electrospun fiber diameter and alignment on macrophage activation and secretion of proinflammatory cytokines and chemokines. / Saino, Enrica; Focarete, Maria Letizia; Gualandi, Chiara; Emanuele, Enzo; Cornaglia, Antonia I.; Imbriani, Marcello; Visai, Livia.

In: Biomacromolecules, Vol. 12, No. 5, 09.05.2011, p. 1900-1911.

Research output: Contribution to journalArticle

Saino, Enrica ; Focarete, Maria Letizia ; Gualandi, Chiara ; Emanuele, Enzo ; Cornaglia, Antonia I. ; Imbriani, Marcello ; Visai, Livia. / Effect of electrospun fiber diameter and alignment on macrophage activation and secretion of proinflammatory cytokines and chemokines. In: Biomacromolecules. 2011 ; Vol. 12, No. 5. pp. 1900-1911.
@article{1931c0732e4c4e1282e8a92747a87f53,
title = "Effect of electrospun fiber diameter and alignment on macrophage activation and secretion of proinflammatory cytokines and chemokines",
abstract = "Macrophage activation can be modulated by biomaterial topography according to the biological scale (micrometric and nanometric range). In this study, we investigated the effect of fiber diameter and fiber alignment of electrospun poly(l-lactic) (PLLA) scaffolds on macrophage RAW 264.7 activation and secretion of proinflammatory cytokines and chemokines at 24 h and 7 days. Macrophages were cultured on four different types of fibrous PLLA scaffold (aligned microfibers, aligned nanofibers, random microfibers, and random nanofibers) and on PLLA film (used as a reference). Substrate topography was found to influence the immune response activated by macrophages, especially in the early inflammation stage. Secretion of proinflammatory molecules by macrophage cells was chiefly dependent on fiber diameter. In particular, nanofibrous PLLA scaffolds minimized the inflammatory response when compared with films and microfibrous scaffolds. The histological evaluation demonstrated a higher number of foreign body giant cells on the PLLA film than on the micro- and nanofibrous scaffolds. In summary, our results indicate that the diameter of electrospun PLLA fibers, rather than fiber alignment, plays a relevant role in influencing in vitro macrophage activation and secretion of proinflammatory molecules.",
author = "Enrica Saino and Focarete, {Maria Letizia} and Chiara Gualandi and Enzo Emanuele and Cornaglia, {Antonia I.} and Marcello Imbriani and Livia Visai",
year = "2011",
month = "5",
day = "9",
doi = "10.1021/bm200248h",
language = "English",
volume = "12",
pages = "1900--1911",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "American Chemical Society",
number = "5",

}

TY - JOUR

T1 - Effect of electrospun fiber diameter and alignment on macrophage activation and secretion of proinflammatory cytokines and chemokines

AU - Saino, Enrica

AU - Focarete, Maria Letizia

AU - Gualandi, Chiara

AU - Emanuele, Enzo

AU - Cornaglia, Antonia I.

AU - Imbriani, Marcello

AU - Visai, Livia

PY - 2011/5/9

Y1 - 2011/5/9

N2 - Macrophage activation can be modulated by biomaterial topography according to the biological scale (micrometric and nanometric range). In this study, we investigated the effect of fiber diameter and fiber alignment of electrospun poly(l-lactic) (PLLA) scaffolds on macrophage RAW 264.7 activation and secretion of proinflammatory cytokines and chemokines at 24 h and 7 days. Macrophages were cultured on four different types of fibrous PLLA scaffold (aligned microfibers, aligned nanofibers, random microfibers, and random nanofibers) and on PLLA film (used as a reference). Substrate topography was found to influence the immune response activated by macrophages, especially in the early inflammation stage. Secretion of proinflammatory molecules by macrophage cells was chiefly dependent on fiber diameter. In particular, nanofibrous PLLA scaffolds minimized the inflammatory response when compared with films and microfibrous scaffolds. The histological evaluation demonstrated a higher number of foreign body giant cells on the PLLA film than on the micro- and nanofibrous scaffolds. In summary, our results indicate that the diameter of electrospun PLLA fibers, rather than fiber alignment, plays a relevant role in influencing in vitro macrophage activation and secretion of proinflammatory molecules.

AB - Macrophage activation can be modulated by biomaterial topography according to the biological scale (micrometric and nanometric range). In this study, we investigated the effect of fiber diameter and fiber alignment of electrospun poly(l-lactic) (PLLA) scaffolds on macrophage RAW 264.7 activation and secretion of proinflammatory cytokines and chemokines at 24 h and 7 days. Macrophages were cultured on four different types of fibrous PLLA scaffold (aligned microfibers, aligned nanofibers, random microfibers, and random nanofibers) and on PLLA film (used as a reference). Substrate topography was found to influence the immune response activated by macrophages, especially in the early inflammation stage. Secretion of proinflammatory molecules by macrophage cells was chiefly dependent on fiber diameter. In particular, nanofibrous PLLA scaffolds minimized the inflammatory response when compared with films and microfibrous scaffolds. The histological evaluation demonstrated a higher number of foreign body giant cells on the PLLA film than on the micro- and nanofibrous scaffolds. In summary, our results indicate that the diameter of electrospun PLLA fibers, rather than fiber alignment, plays a relevant role in influencing in vitro macrophage activation and secretion of proinflammatory molecules.

UR - http://www.scopus.com/inward/record.url?scp=79955816318&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955816318&partnerID=8YFLogxK

U2 - 10.1021/bm200248h

DO - 10.1021/bm200248h

M3 - Article

VL - 12

SP - 1900

EP - 1911

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 5

ER -