Effect of endothelial cell conditioned medium on the growth of human bone marrow fibroblasts

G. B. Scarra, P. Fiorentini, G. Garbarino

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Both endothelial cells (EC) and fibroblasts, two discrete populations of hemopoietic stroma, are known to modulate the proliferation and differentiation of hemopoietic progenitors. Recent reports also demonstrated that EC stimulate the in vitro growth of fibroblasts via a soluble factor. This finding seems to support the hypothesis that EC may play a role in the pathogenesis of bone marrow fibrosis in myeloproliferative disorders (MD). We have studied the effects of the conditioned medium (CM) from human umbilical vein EC cultures, obtained in serum free conditions, on the growth of bone marrow fibroblasts from normal donors and from patients with MD. The results show that EC derived CM contains a factor which stimulates the proliferation of fibroblasts and that can act as an authentic growth factor by inducing 'quiescent' fibroblasts to proliferate. Moreover, we found that this endothelial derived growth factor (EDGF) equally promotes the proliferation of both normal and pathological progenitors of bone marrow fibroblasts (CFU-F) by increasing both the number and the size of the colonies.

Original languageEnglish
Pages (from-to)343-346
Number of pages4
JournalJournal of Cellular Physiology
Volume123
Issue number3
Publication statusPublished - 1985

Fingerprint

Endothelial cells
Fibroblasts
Conditioned Culture Medium
Bone
Endothelial Cells
Bone Marrow
Growth
Myeloproliferative Disorders
Intercellular Signaling Peptides and Proteins
Endothelial Growth Factors
Primary Myelofibrosis
Human Umbilical Vein Endothelial Cells
Cell culture
Cell Culture Techniques
Tissue Donors
Serum
Population

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Effect of endothelial cell conditioned medium on the growth of human bone marrow fibroblasts. / Scarra, G. B.; Fiorentini, P.; Garbarino, G.

In: Journal of Cellular Physiology, Vol. 123, No. 3, 1985, p. 343-346.

Research output: Contribution to journalArticle

Scarra, GB, Fiorentini, P & Garbarino, G 1985, 'Effect of endothelial cell conditioned medium on the growth of human bone marrow fibroblasts', Journal of Cellular Physiology, vol. 123, no. 3, pp. 343-346.
Scarra, G. B. ; Fiorentini, P. ; Garbarino, G. / Effect of endothelial cell conditioned medium on the growth of human bone marrow fibroblasts. In: Journal of Cellular Physiology. 1985 ; Vol. 123, No. 3. pp. 343-346.
@article{d9e7290daa0d423c8a5c878809571f61,
title = "Effect of endothelial cell conditioned medium on the growth of human bone marrow fibroblasts",
abstract = "Both endothelial cells (EC) and fibroblasts, two discrete populations of hemopoietic stroma, are known to modulate the proliferation and differentiation of hemopoietic progenitors. Recent reports also demonstrated that EC stimulate the in vitro growth of fibroblasts via a soluble factor. This finding seems to support the hypothesis that EC may play a role in the pathogenesis of bone marrow fibrosis in myeloproliferative disorders (MD). We have studied the effects of the conditioned medium (CM) from human umbilical vein EC cultures, obtained in serum free conditions, on the growth of bone marrow fibroblasts from normal donors and from patients with MD. The results show that EC derived CM contains a factor which stimulates the proliferation of fibroblasts and that can act as an authentic growth factor by inducing 'quiescent' fibroblasts to proliferate. Moreover, we found that this endothelial derived growth factor (EDGF) equally promotes the proliferation of both normal and pathological progenitors of bone marrow fibroblasts (CFU-F) by increasing both the number and the size of the colonies.",
author = "Scarra, {G. B.} and P. Fiorentini and G. Garbarino",
year = "1985",
language = "English",
volume = "123",
pages = "343--346",
journal = "Journal of cellular and comparative physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Effect of endothelial cell conditioned medium on the growth of human bone marrow fibroblasts

AU - Scarra, G. B.

AU - Fiorentini, P.

AU - Garbarino, G.

PY - 1985

Y1 - 1985

N2 - Both endothelial cells (EC) and fibroblasts, two discrete populations of hemopoietic stroma, are known to modulate the proliferation and differentiation of hemopoietic progenitors. Recent reports also demonstrated that EC stimulate the in vitro growth of fibroblasts via a soluble factor. This finding seems to support the hypothesis that EC may play a role in the pathogenesis of bone marrow fibrosis in myeloproliferative disorders (MD). We have studied the effects of the conditioned medium (CM) from human umbilical vein EC cultures, obtained in serum free conditions, on the growth of bone marrow fibroblasts from normal donors and from patients with MD. The results show that EC derived CM contains a factor which stimulates the proliferation of fibroblasts and that can act as an authentic growth factor by inducing 'quiescent' fibroblasts to proliferate. Moreover, we found that this endothelial derived growth factor (EDGF) equally promotes the proliferation of both normal and pathological progenitors of bone marrow fibroblasts (CFU-F) by increasing both the number and the size of the colonies.

AB - Both endothelial cells (EC) and fibroblasts, two discrete populations of hemopoietic stroma, are known to modulate the proliferation and differentiation of hemopoietic progenitors. Recent reports also demonstrated that EC stimulate the in vitro growth of fibroblasts via a soluble factor. This finding seems to support the hypothesis that EC may play a role in the pathogenesis of bone marrow fibrosis in myeloproliferative disorders (MD). We have studied the effects of the conditioned medium (CM) from human umbilical vein EC cultures, obtained in serum free conditions, on the growth of bone marrow fibroblasts from normal donors and from patients with MD. The results show that EC derived CM contains a factor which stimulates the proliferation of fibroblasts and that can act as an authentic growth factor by inducing 'quiescent' fibroblasts to proliferate. Moreover, we found that this endothelial derived growth factor (EDGF) equally promotes the proliferation of both normal and pathological progenitors of bone marrow fibroblasts (CFU-F) by increasing both the number and the size of the colonies.

UR - http://www.scopus.com/inward/record.url?scp=0021826682&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021826682&partnerID=8YFLogxK

M3 - Article

C2 - 3988811

AN - SCOPUS:0021826682

VL - 123

SP - 343

EP - 346

JO - Journal of cellular and comparative physiology

JF - Journal of cellular and comparative physiology

SN - 0021-9541

IS - 3

ER -