Effect of ethanol and red wine on ochratoxin A-induced experimental acute nephrotoxicity

Alberto A E Bertelli, Massimiliano Migliori, Cristina Filippi, Nicoletta Gagliano, Elena Donetti, Vincenzo Panichi, Vera Scalori, Renzo Colombo, Claudio Mannari, Jean Paul Tillement, Luca Giovannini

Research output: Contribution to journalArticlepeer-review

Abstract

Ochratoxin A (OTA), is a nephrotoxic mycotoxin present in wine, which is nephrotoxic in humans. Our working hypothesis is that natural substances in wine may counteract OTA toxicity. Thirty-six rats were randomized to OTA dissolved in saline, red wine, or 13.5% ethanol or to OTA-free wine, ethanol, or saline. OTA (289 μg/kg of body weight/48 h) was administered by gastric gavage for 2 weeks. Serum creatinine, tubular enzymuria, renal lipohydroperoxides (LOOH), reduced (GSH) and oxidized (GSSG) glutathione, and renal superoxide dismutase activity (SOD) were determined in renal tissue. OTA alone produced significant increases in renal lipoperoxides and significant decreases in SOD and GSH/GSSG ratio. In red wine or ethanol, OTA was less nephrotoxic, reducing oxidative damage as revealed by LOOH. In OTA-wine and OTA-ethanol groups, SOD activity was higher than in the OTA-treated one, suggesting that both ethanol and nonalcoholic fractions may preserve antioxidant reserve. GSH/GSSG ratio was significantly preserved only in the OTA-wine group and not in OTA-ethanol. Red wine may exert a protective effect against OTA nephrotoxicity by limiting oxidative damage. The ostensible protection afforded by ethanol deserves further investigation.

Original languageEnglish
Pages (from-to)6924-6929
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume53
Issue number17
DOIs
Publication statusPublished - Aug 24 2005

Keywords

  • Ethanol
  • Glutathione
  • Lipid peroxidation
  • Nephrotoxicity
  • Ochratoxin A
  • Rat
  • Red wine
  • Superoxide dismutase activity

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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