Effect of fibroblasts on the growth of erythroid progenitor cells in vitro

R. Ghio, G. Bianchi, B. Lowenberg

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effect of embryo fibroblasts on the growth of erythroid colony-forming cells in vitro (CFU-e) from mouse bone marrow was investigated. First, the maintenance of CFU-e number in suspension culture was assayed. CFU-e recovered from suspension culture fell rapidly to values below 30% of the initial number. When erythropoietin (EP) was added, the initial decline during the first day was followed by a rise to 80%. In cultures supplemented with irradiated fibroblasts, the number of CFU-e did not show an abortive fall, but there was a slight increase during 3 days of culturing. The influence of fibroblasts on the colony-forming ability of CFU-e was studied in a semisolid culture system composed of an agar underlayer and a methylcellulose overlayer. The number of erythroid colonies scored after 5 days of culture in the presence of different levels of EP was proportional to the number of added fibroblasts and the colony size (depending on the number of fibroblasts) increased to macroscopic dimensions. Fibroblasts alone, without EP, induced colony formation by CFU-e if added in concentrations of 1 x 105 or higher. EP was not detectable in medium conditioned by the fibroblasts. These data indicate that fibroblasts may stimulate erythroid colony formation (in the absence of EP) and enhance the colony-forming ability of CFU-e in the presence of EP. From these results, it is suggested that fibroblasts exert proliferation activating effects on CFU-e target cells.

Original languageEnglish
Pages (from-to)341-347
Number of pages7
JournalExperimental Hematology
Volume5
Issue number5
Publication statusPublished - 1977

Fingerprint

Erythroid Precursor Cells
Fibroblasts
Erythropoietin
Growth
Suspensions
In Vitro Techniques
Methylcellulose
Conditioned Culture Medium
Agar
Embryonic Structures
Bone Marrow
Maintenance

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Ghio, R., Bianchi, G., & Lowenberg, B. (1977). Effect of fibroblasts on the growth of erythroid progenitor cells in vitro. Experimental Hematology, 5(5), 341-347.

Effect of fibroblasts on the growth of erythroid progenitor cells in vitro. / Ghio, R.; Bianchi, G.; Lowenberg, B.

In: Experimental Hematology, Vol. 5, No. 5, 1977, p. 341-347.

Research output: Contribution to journalArticle

Ghio, R, Bianchi, G & Lowenberg, B 1977, 'Effect of fibroblasts on the growth of erythroid progenitor cells in vitro', Experimental Hematology, vol. 5, no. 5, pp. 341-347.
Ghio, R. ; Bianchi, G. ; Lowenberg, B. / Effect of fibroblasts on the growth of erythroid progenitor cells in vitro. In: Experimental Hematology. 1977 ; Vol. 5, No. 5. pp. 341-347.
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