Based on the hypothesis that bone cements cause changes in the production of transforming growth factor-beta 1 (TGF-β1) by bone cells, the effects of four acrylic bone cements (Sulfix-60, CMW 1, CMW 2 and CMW 3) were examined using the osteoblast-like cell line MG63. The extracts in MEM of the cements were tested, following 1h- and 7 day-curing. MG63 cells seldom expressed mRNA specific for TGF-β1 in basal conditions. The cultures expressed mRNA constantly after incubation with the extract of CMW 1 cured for 1h. TGF-β1 specific mRNA was seldom expressed after incubation with the other cement extracts. The release of TGF-β1 into the conditioned medium was increased significantly by CMW 1 extract at 1h-curing, but was not changed significantly by CMW 1 extract at 7 day-curing and by the extracts of the other cements, at both curing times. The stimulating effect of CMW 1 on the secretion of TGF-β1, even with all the restrictions of an in vitro study of continuous cell lines, if confirmed in vivo, might favor the development of the synovial-like membrane around the implant, and therefore impair the chance of success of the prosthesis.
- Transforming growth factor-beta 1
ASJC Scopus subject areas
- Biomedical Engineering