Effect of four acrylic bone cements on transforming growth factor-beta1 expression by osteoblast-like cells MG63

Elisabetta Cenni, Donatella Granchi, Gabriela Ciapetti, Lucia Savarino, Alessandra Corradini

Research output: Contribution to journalArticle

Abstract

Based on the hypothesis that bone cements cause changes in the production of transforming growth factor-beta 1 (TGF-β1) by bone cells, the effects of four acrylic bone cements (Sulfix-60, CMW 1, CMW 2 and CMW 3) were examined using the osteoblast-like cell line MG63. The extracts in MEM of the cements were tested, following 1h- and 7 day-curing. MG63 cells seldom expressed mRNA specific for TGF-β1 in basal conditions. The cultures expressed mRNA constantly after incubation with the extract of CMW 1 cured for 1h. TGF-β1 specific mRNA was seldom expressed after incubation with the other cement extracts. The release of TGF-β1 into the conditioned medium was increased significantly by CMW 1 extract at 1h-curing, but was not changed significantly by CMW 1 extract at 7 day-curing and by the extracts of the other cements, at both curing times. The stimulating effect of CMW 1 on the secretion of TGF-β1, even with all the restrictions of an in vitro study of continuous cell lines, if confirmed in vivo, might favor the development of the synovial-like membrane around the implant, and therefore impair the chance of success of the prosthesis.

Original languageEnglish
Pages (from-to)305-311
Number of pages7
JournalBiomaterials
Volume23
Issue number1
DOIs
Publication statusPublished - Jan 1 2002

Keywords

  • Cement
  • MG63
  • Osteoblast
  • Transforming growth factor-beta 1

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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