Effect of genistein on cholesterol metabolism-related genes in a colon cancer cell line

Maria Notarnicola, Caterina Messa, Antonella Orlando, Benedetta D'Attoma, Valeria Tutino, Rosemary Rivizzigno, Maria Gabriella Caruso

Research output: Contribution to journalArticlepeer-review

Abstract

The major soy-derived isoflavones such as genistein has been demonstrated to possess anticarcinogenic activity in animal model systems. The present study was designed to investigate the effects of isoflavone genistein exposure at concentrations ranging from 0.01 to 50 μM on the LDL receptor and HMG-CoA reductase gene expression in the estrogen receptor positive DLD-1 human colon cancer cell line. LDL receptor and HMG-CoA reductase gene expressions were evaluated by reverse transcription followed by real-time PCR. Genistein induced an increase of LDL receptor gene expression and later decrease of HMG-CoA reductase mRNA expression in DLD-1 cells. These findings provide direct evidence on the role of genistein in regulating LDL receptor and HMG-CoA reductase gene expression in colon cancer.

Original languageEnglish
Pages (from-to)35-40
Number of pages6
JournalGenes and Nutrition
Volume3
Issue number1
DOIs
Publication statusPublished - 2008

Keywords

  • Colon cancer
  • Genistein
  • HMG-CoA reductase
  • LDL receptor

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Genetics

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