TY - JOUR
T1 - Effect of growth hormone therapy on the proinflammatory cytokine profile in growth hormone-deficient children
AU - Pagani, Sara
AU - Meazza, Cristina
AU - Travaglino, Paola
AU - Moretta, Antonia
AU - Bozzola, Mauro
PY - 2005/1
Y1 - 2005/1
N2 - The aim of the present study was to establish whether growth hormone (GH) treatment in vivo affects pro-inflammatory cytokine production by resting or in vitro, activated, cultured, peripheral blood mononuclear cells (PBMC) from children with complete growth hormone deficiency (GHD). We evaluated 11, pre-pubertal children (6 males and 5 females) with GHD, aged between 6 and 14 years, and 9, age- and sex-matched healthy subjects were studied as controls (CTRLs). Freshly isolated PBMC were cultured for 4 or 24 h in X-VIVO medium in the presence or absence of 0.01 μg/mL lipopolysaccharide for the determination of TNF-α and IL-6 production; alternatively, cells were incubated 24 h in X-VIVO medium with or without 25 μg/mL Concanavalin A for IFN-γ production. Cytokines were measured in the cell supernatants by enzyme-linked immunosorbent assay kits. The results of the present study provide evidence that spontaneous and/or mitogen-induced, in vitro PBMC production of pro-inflammatory cytokines is lower in GHD children than in healthy, age-matched individuals (p
AB - The aim of the present study was to establish whether growth hormone (GH) treatment in vivo affects pro-inflammatory cytokine production by resting or in vitro, activated, cultured, peripheral blood mononuclear cells (PBMC) from children with complete growth hormone deficiency (GHD). We evaluated 11, pre-pubertal children (6 males and 5 females) with GHD, aged between 6 and 14 years, and 9, age- and sex-matched healthy subjects were studied as controls (CTRLs). Freshly isolated PBMC were cultured for 4 or 24 h in X-VIVO medium in the presence or absence of 0.01 μg/mL lipopolysaccharide for the determination of TNF-α and IL-6 production; alternatively, cells were incubated 24 h in X-VIVO medium with or without 25 μg/mL Concanavalin A for IFN-γ production. Cytokines were measured in the cell supernatants by enzyme-linked immunosorbent assay kits. The results of the present study provide evidence that spontaneous and/or mitogen-induced, in vitro PBMC production of pro-inflammatory cytokines is lower in GHD children than in healthy, age-matched individuals (p
KW - Growth hormone deficiency
KW - Immune system
KW - Peripheral blood mononuclear cells
KW - Proinflammatory cytokines
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M3 - Article
C2 - 15809208
AN - SCOPUS:16444363957
VL - 16
SP - 65
EP - 69
JO - Environmental and Molecular Mutagenesis
JF - Environmental and Molecular Mutagenesis
SN - 0893-6692
IS - 1
ER -