Effect of growth hormone therapy on the proinflammatory cytokine profile in growth hormone-deficient children

Sara Pagani, Cristina Meazza, Paola Travaglino, Antonia Moretta, Mauro Bozzola

Research output: Contribution to journalArticle

Abstract

The aim of the present study was to establish whether growth hormone (GH) treatment in vivo affects pro-inflammatory cytokine production by resting or in vitro, activated, cultured, peripheral blood mononuclear cells (PBMC) from children with complete growth hormone deficiency (GHD). We evaluated 11, pre-pubertal children (6 males and 5 females) with GHD, aged between 6 and 14 years, and 9, age- and sex-matched healthy subjects were studied as controls (CTRLs). Freshly isolated PBMC were cultured for 4 or 24 h in X-VIVO medium in the presence or absence of 0.01 μg/mL lipopolysaccharide for the determination of TNF-α and IL-6 production; alternatively, cells were incubated 24 h in X-VIVO medium with or without 25 μg/mL Concanavalin A for IFN-γ production. Cytokines were measured in the cell supernatants by enzyme-linked immunosorbent assay kits. The results of the present study provide evidence that spontaneous and/or mitogen-induced, in vitro PBMC production of pro-inflammatory cytokines is lower in GHD children than in healthy, age-matched individuals (p

Original languageEnglish
Pages (from-to)65-69
Number of pages5
JournalEuropean Cytokine Network
Volume16
Issue number1
Publication statusPublished - Jan 2005

Keywords

  • Growth hormone deficiency
  • Immune system
  • Peripheral blood mononuclear cells
  • Proinflammatory cytokines

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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