Effect of Helicobacter pylori lipopolysaccharide (LPS) and LPS derivatives on the production of tissue factor and plasminogen activator inhibitor type 2 by human blood mononuclear cells

Nicola Semeraro, Pasqualina Montemurro, Claudia Piccoli, Vito Muolo, Mario Colucci, Giuseppe Giuliani, Donato Fumarola, Salvatore Pece, Anthony P. Moran

Research output: Contribution to journalArticle

Abstract

Different Helicobacter pylori lipopolysaccharides (LPSs) and LPS- derivatives were studied for their ability to induce the production of procoagulant activity (PCA) and plasminogen activator inhibitor type 2 (PAI- 2) by human blood mononuclear leukocytes. Smooth (S)- and rough (R)-form LPSs caused a similar increase in cell-associated PCA (tissue factor) and PAI-2 antigen release. Both effects were potentiated by fetal bovine serum via a CD14-mediated mechanism. The potency of H. pylori LPSs was ~1000-fold lower than that of Salmonella typhimurium LPSs. When H. pylori LPS derivatives (dephosphorylated R-LPS, S-lipid A, and R-lipid A) were used, PCA and PAI-2 production were markedly reduced. R-lipid A was ~4-fold less efficient than S-lipid A. These findings suggest that the induction of monocyte tissue factor and PAI-2 by H. pylori LPS is influenced by the lipid A structure and modulated by the core oligosaccharide and that phosphate groups present in both regions may play an important role.

Original languageEnglish
Pages (from-to)1255-1260
Number of pages6
JournalJournal of Infectious Diseases
Volume174
Issue number6
Publication statusPublished - 1996

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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