Effect of hemopoietic growth factors on the proliferation of acute myeloid and lymphoid leukemias

Massimo Aglietta, Lidia de Felice, Alessandra Stacchini, Fiorella Sanavio, Antonella Severino, Francesca Simone, Wanda Piacibello, Franco Mandelli

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of G-CSF, GM-CSF, IL-1 and IL-3 on the proliferation of acute leukemia cells (evaluated as 3HTdR uptake) was investigated in short-term liquid cultures and compared with that observed on normal bone marrow (BM) populations enriched for immature cells. In acute myeloid leukemias (AML), a marked leukemic proliferation was induced in 10/18 cases by IL-3, in 9/18 by GM-CSF, in 7/18 by IL-1 and in 4/18 by G-CSF. In acute lymphoid leukemias (ALL), marked stimulation was observed in 7/11 cases with IL-3 and in 5/11 with GM-CSF, whereas IL-1 and G-CSF were ineffective. Both in AML and ALL, the combination of several factors did not result in an additive synergistic effect. Purified normal BM cells responded to all four growth factors and their combinations produced an additive effect on cell proliferation which probably relates to the heterogeneity of the cell populations studied. The effect of a G-CSF, GM-CSF, IL-1 and IL-3 on the proliferation of acute leukemia cells by different growth factors suggests that caution should be exercised in their clinical use in these diseases

Original languageEnglish
Pages (from-to)207-214
Number of pages8
JournalLeukemia and Lymphoma
Volume2
Issue number3-4
DOIs
Publication statusPublished - 1990

Keywords

  • Cell kinetics
  • Differentiation
  • Growth factors
  • In vitro
  • Leukemia

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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