Abstract
Recent studies have demonstrated that Apo AIV exerts a protective effect against atherosclerosis. Moreover, Qin et al. (Am. J. Physiol. 274 (1998) H1836) have demonstrated that Apo AIV, isolated from rat plasma, exerts an inhibitory effect against Cu2+ -induced lipid peroxidation of intestinal lymph and LDL. The aim of the study was to investigate whether human Apo AIV exerts a protective effect against Cu2+ -induced lipid peroxidation. Our results demonstrated that human Apo AIV exerted an inhibitory effect against Cu2+ and AAPH induced lipid peroxidation of VLDL, as shown by the lower increase in the levels of TBARS and conjugated dienes in lipoproteins preincubated with Apo AIV. In addition, the tryptophan (Trp) and probe 2-(dimethylamino)-6-lauroylnaphthalene (Laurdan) fluorescence studies demonstrated that the modifications of spectral properties in both lipoproteins preincubated with Apo AIV were lower with respect to ox-lipoproteins, suggesting that Apo AIV prevents the modification of physico-chemical properties due to peroxidation.
| Original language | English |
|---|---|
| Pages (from-to) | 45-54 |
| Number of pages | 10 |
| Journal | Chemistry and Physics of Lipids |
| Volume | 114 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2002 |
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Keywords
- Apo AIV
- Conjugated dienes
- Fluorescence
- Lipoprotein peroxidation
- Oxidative damage
- TBARS
ASJC Scopus subject areas
- Biochemistry
- Biophysics
Cite this
Effect of human Apo AIV against lipid peroxidation of very low density lipoproteins. / Ferretti, G.; Bacchetti, T.; Bicchiega, V.; Curatola, G.
In: Chemistry and Physics of Lipids, Vol. 114, No. 1, 2002, p. 45-54.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of human Apo AIV against lipid peroxidation of very low density lipoproteins
AU - Ferretti, G.
AU - Bacchetti, T.
AU - Bicchiega, V.
AU - Curatola, G.
PY - 2002
Y1 - 2002
N2 - Recent studies have demonstrated that Apo AIV exerts a protective effect against atherosclerosis. Moreover, Qin et al. (Am. J. Physiol. 274 (1998) H1836) have demonstrated that Apo AIV, isolated from rat plasma, exerts an inhibitory effect against Cu2+ -induced lipid peroxidation of intestinal lymph and LDL. The aim of the study was to investigate whether human Apo AIV exerts a protective effect against Cu2+ -induced lipid peroxidation. Our results demonstrated that human Apo AIV exerted an inhibitory effect against Cu2+ and AAPH induced lipid peroxidation of VLDL, as shown by the lower increase in the levels of TBARS and conjugated dienes in lipoproteins preincubated with Apo AIV. In addition, the tryptophan (Trp) and probe 2-(dimethylamino)-6-lauroylnaphthalene (Laurdan) fluorescence studies demonstrated that the modifications of spectral properties in both lipoproteins preincubated with Apo AIV were lower with respect to ox-lipoproteins, suggesting that Apo AIV prevents the modification of physico-chemical properties due to peroxidation.
AB - Recent studies have demonstrated that Apo AIV exerts a protective effect against atherosclerosis. Moreover, Qin et al. (Am. J. Physiol. 274 (1998) H1836) have demonstrated that Apo AIV, isolated from rat plasma, exerts an inhibitory effect against Cu2+ -induced lipid peroxidation of intestinal lymph and LDL. The aim of the study was to investigate whether human Apo AIV exerts a protective effect against Cu2+ -induced lipid peroxidation. Our results demonstrated that human Apo AIV exerted an inhibitory effect against Cu2+ and AAPH induced lipid peroxidation of VLDL, as shown by the lower increase in the levels of TBARS and conjugated dienes in lipoproteins preincubated with Apo AIV. In addition, the tryptophan (Trp) and probe 2-(dimethylamino)-6-lauroylnaphthalene (Laurdan) fluorescence studies demonstrated that the modifications of spectral properties in both lipoproteins preincubated with Apo AIV were lower with respect to ox-lipoproteins, suggesting that Apo AIV prevents the modification of physico-chemical properties due to peroxidation.
KW - Apo AIV
KW - Conjugated dienes
KW - Fluorescence
KW - Lipoprotein peroxidation
KW - Oxidative damage
KW - TBARS
UR - http://www.scopus.com/inward/record.url?scp=0036008311&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036008311&partnerID=8YFLogxK
U2 - 10.1016/S0009-3084(01)00201-8
DO - 10.1016/S0009-3084(01)00201-8
M3 - Article
C2 - 11841825
AN - SCOPUS:0036008311
VL - 114
SP - 45
EP - 54
JO - Chemistry and Physics of Lipids
JF - Chemistry and Physics of Lipids
SN - 0009-3084
IS - 1
ER -