Effect of human Apo AIV against lipid peroxidation of very low density lipoproteins

G. Ferretti, T. Bacchetti, V. Bicchiega, G. Curatola

Research output: Contribution to journalArticlepeer-review

Abstract

Recent studies have demonstrated that Apo AIV exerts a protective effect against atherosclerosis. Moreover, Qin et al. (Am. J. Physiol. 274 (1998) H1836) have demonstrated that Apo AIV, isolated from rat plasma, exerts an inhibitory effect against Cu2+ -induced lipid peroxidation of intestinal lymph and LDL. The aim of the study was to investigate whether human Apo AIV exerts a protective effect against Cu2+ -induced lipid peroxidation. Our results demonstrated that human Apo AIV exerted an inhibitory effect against Cu2+ and AAPH induced lipid peroxidation of VLDL, as shown by the lower increase in the levels of TBARS and conjugated dienes in lipoproteins preincubated with Apo AIV. In addition, the tryptophan (Trp) and probe 2-(dimethylamino)-6-lauroylnaphthalene (Laurdan) fluorescence studies demonstrated that the modifications of spectral properties in both lipoproteins preincubated with Apo AIV were lower with respect to ox-lipoproteins, suggesting that Apo AIV prevents the modification of physico-chemical properties due to peroxidation.

Original languageEnglish
Pages (from-to)45-54
Number of pages10
JournalChemistry and Physics of Lipids
Volume114
Issue number1
DOIs
Publication statusPublished - 2002

Keywords

  • Apo AIV
  • Conjugated dienes
  • Fluorescence
  • Lipoprotein peroxidation
  • Oxidative damage
  • TBARS

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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