Effect of human recombinant interferon on cytotoxic activity of natural killer (NK) cells and monocytes

Ronald B. Herberman, John R. Ortaldo, Alberto Mantovani, Donna S. Hobbs, Hsiang fu Kung, Sidney Pestka

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Abstract

Studies have been performed on the in vitro immunologic effects of homogeneous recombinant human leukocyte interferon, IFLrA. Large granular lymphocytes, enriched for natural killer (NK) cell activity, were pretreated wtih IFLrA or natural interferon preparations and then tested for augmentation of NK activity and of antibody-dependent cell-mediated cytoxicity (ADCC). Monocytes were tested for cytolytic and cytostatic activity in 48-72 hr radioisotopic assays performed in the presence or absence of interferon. Treatment with IFLrA caused significant augmentation of NK, ADCC, and monocyte-mediated cytotoxic activities. Even 10 units of IFLrA induced augmentation of NK activity, and 100 units or more boosted monocyte-mediated activity. The effects in each of these assays were species-specific, with no detectable effects on the activity of mouse effector cells. These results indicate that homogeneous recombinant interferon has potent in vitro immunomodulating effects and thus provide a basis for carefully examining the in vivo effects of this protein on host defenses in forthcoming clinical trials with cancer patients.

Original languageEnglish
Pages (from-to)160-167
Number of pages8
JournalCellular Immunology
Volume67
Issue number1
DOIs
Publication statusPublished - 1982

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Natural Killer Cells
Interferons
Antibody-Dependent Cell Cytotoxicity
Monocytes
Cytostatic Agents
Interferon-alpha
Clinical Trials
Lymphocytes
Neoplasms
Proteins
In Vitro Techniques
Therapeutics

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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Effect of human recombinant interferon on cytotoxic activity of natural killer (NK) cells and monocytes. / Herberman, Ronald B.; Ortaldo, John R.; Mantovani, Alberto; Hobbs, Donna S.; Kung, Hsiang fu; Pestka, Sidney.

In: Cellular Immunology, Vol. 67, No. 1, 1982, p. 160-167.

Research output: Contribution to journalArticle

Herberman, Ronald B. ; Ortaldo, John R. ; Mantovani, Alberto ; Hobbs, Donna S. ; Kung, Hsiang fu ; Pestka, Sidney. / Effect of human recombinant interferon on cytotoxic activity of natural killer (NK) cells and monocytes. In: Cellular Immunology. 1982 ; Vol. 67, No. 1. pp. 160-167.
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