Effect of hyperglycemia on the number of CD117(+) progenitor cells and their differentiation toward endothelial progenitor cells in young and old ages

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Abstract

Dysfunction of endothelial progenitor cells (EPCs) has been reported either in aging or diabetes, though the influence of an "old" environment on numerical and functional changes of diabetes associated EPCs is not known. We evaluated the effect of both aging and early stage of streptozotocin-induced diabetes on the number of bone marrow-derived CD117(+) progenitor cells, and on their differentiation in vitro toward EPCs. The phenotype of progenitor cells and the uptake of acetylated-low density lipoprotein (Ac-LDL) were evaluated after cell culture in VEGF, FGF-1, and IGF-1 supplemented medium. Hyperglycemia similarly reduced the number of CD117(+) cells both in young and old mice. CD117(+) cells from young mice differentiated better than those from old animals "in vitro", with a greater reduction of CD117(+) cells and an higher increase of CD184(+)VEGFR-2(+) cells. In diabetic mice, in vitro CD117(+) cells differentiation was significantly reduced in young animals. Diabetes did not impact on the scarce differentiation of CD117(+) cells from old mice. Hyperglycemia reduced the uptake of acLDL by EPCs greatly in young than in old mice. These findings indicate that part of the EPCs functional alterations induced by hyperglicemia in young mice are observed in normal aged mice.

Original languageEnglish
Pages (from-to)31-36
Number of pages6
JournalMechanisms of Ageing and Development
Volume159
DOIs
Publication statusPublished - Oct 2016

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Hyperglycemia
Cell Differentiation
Stem Cells
Fibroblast Growth Factor 1
Vascular Endothelial Growth Factor Receptor-2
Experimental Diabetes Mellitus
Endothelial Progenitor Cells
Insulin-Like Growth Factor I
Vascular Endothelial Growth Factor A
Cell Culture Techniques
Cell Count
Bone Marrow
Phenotype
In Vitro Techniques

Keywords

  • Journal Article

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title = "Effect of hyperglycemia on the number of CD117(+) progenitor cells and their differentiation toward endothelial progenitor cells in young and old ages",
abstract = "Dysfunction of endothelial progenitor cells (EPCs) has been reported either in aging or diabetes, though the influence of an {"}old{"} environment on numerical and functional changes of diabetes associated EPCs is not known. We evaluated the effect of both aging and early stage of streptozotocin-induced diabetes on the number of bone marrow-derived CD117(+) progenitor cells, and on their differentiation in vitro toward EPCs. The phenotype of progenitor cells and the uptake of acetylated-low density lipoprotein (Ac-LDL) were evaluated after cell culture in VEGF, FGF-1, and IGF-1 supplemented medium. Hyperglycemia similarly reduced the number of CD117(+) cells both in young and old mice. CD117(+) cells from young mice differentiated better than those from old animals {"}in vitro{"}, with a greater reduction of CD117(+) cells and an higher increase of CD184(+)VEGFR-2(+) cells. In diabetic mice, in vitro CD117(+) cells differentiation was significantly reduced in young animals. Diabetes did not impact on the scarce differentiation of CD117(+) cells from old mice. Hyperglycemia reduced the uptake of acLDL by EPCs greatly in young than in old mice. These findings indicate that part of the EPCs functional alterations induced by hyperglicemia in young mice are observed in normal aged mice.",
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author = "Elisa Pierpaoli and Raffaella Moresi and Fiorenza Orlando and Marco Malavolta and Mauro Provinciali",
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year = "2016",
month = "10",
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language = "English",
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T1 - Effect of hyperglycemia on the number of CD117(+) progenitor cells and their differentiation toward endothelial progenitor cells in young and old ages

AU - Pierpaoli, Elisa

AU - Moresi, Raffaella

AU - Orlando, Fiorenza

AU - Malavolta, Marco

AU - Provinciali, Mauro

N1 - Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

PY - 2016/10

Y1 - 2016/10

N2 - Dysfunction of endothelial progenitor cells (EPCs) has been reported either in aging or diabetes, though the influence of an "old" environment on numerical and functional changes of diabetes associated EPCs is not known. We evaluated the effect of both aging and early stage of streptozotocin-induced diabetes on the number of bone marrow-derived CD117(+) progenitor cells, and on their differentiation in vitro toward EPCs. The phenotype of progenitor cells and the uptake of acetylated-low density lipoprotein (Ac-LDL) were evaluated after cell culture in VEGF, FGF-1, and IGF-1 supplemented medium. Hyperglycemia similarly reduced the number of CD117(+) cells both in young and old mice. CD117(+) cells from young mice differentiated better than those from old animals "in vitro", with a greater reduction of CD117(+) cells and an higher increase of CD184(+)VEGFR-2(+) cells. In diabetic mice, in vitro CD117(+) cells differentiation was significantly reduced in young animals. Diabetes did not impact on the scarce differentiation of CD117(+) cells from old mice. Hyperglycemia reduced the uptake of acLDL by EPCs greatly in young than in old mice. These findings indicate that part of the EPCs functional alterations induced by hyperglicemia in young mice are observed in normal aged mice.

AB - Dysfunction of endothelial progenitor cells (EPCs) has been reported either in aging or diabetes, though the influence of an "old" environment on numerical and functional changes of diabetes associated EPCs is not known. We evaluated the effect of both aging and early stage of streptozotocin-induced diabetes on the number of bone marrow-derived CD117(+) progenitor cells, and on their differentiation in vitro toward EPCs. The phenotype of progenitor cells and the uptake of acetylated-low density lipoprotein (Ac-LDL) were evaluated after cell culture in VEGF, FGF-1, and IGF-1 supplemented medium. Hyperglycemia similarly reduced the number of CD117(+) cells both in young and old mice. CD117(+) cells from young mice differentiated better than those from old animals "in vitro", with a greater reduction of CD117(+) cells and an higher increase of CD184(+)VEGFR-2(+) cells. In diabetic mice, in vitro CD117(+) cells differentiation was significantly reduced in young animals. Diabetes did not impact on the scarce differentiation of CD117(+) cells from old mice. Hyperglycemia reduced the uptake of acLDL by EPCs greatly in young than in old mice. These findings indicate that part of the EPCs functional alterations induced by hyperglicemia in young mice are observed in normal aged mice.

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DO - 10.1016/j.mad.2016.02.006

M3 - Article

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VL - 159

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JO - Mechanisms of Ageing and Development

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