Effect of hyperthermia on isolated perfused rat liver

G. Zavagno, D. Vespa, N. Moschin, C. Belluco, A. Cecchetto, E. Bertocco, A. Raimondo, M. Lise

Research output: Contribution to journalArticle

Abstract

Four groups of isolated rat liver underwent perfusion at 37°C for 1 h, at 37, 40, 43 or 45°C, respectively, during the 2nd hour and then at 37°C again for the 3rd hour. Vascular resistance slightly decreased during hyperthermia and then significantly increased after restarting normothermic perfusion. At 40°C bile production, oxygen consumption, glucose and lactate release did not significantly differ from those found in the 37°C group. At 43 and 45°C all these parameters were significantly impaired when compared to the 37 and 40°C groups and did not recover after normothermic perfusion was restored. GOT and GPT release increased in proportion to the temperature. Microscopic examination revealed normal histologic features in 37 and 40°C specimens while alterations such as vacuolization and focal necrosis were found in the 43 and 45°C groups. These data indicate that the highest temperature that is well tolerated by isolated rat livers for 1 h is located between 40 and 43°C.

Original languageEnglish
Pages (from-to)243-250
Number of pages8
JournalEuropean Surgical Research
Volume21
Issue number5
Publication statusPublished - 1989

Fingerprint

Fever
Perfusion
Liver
Temperature
Bile
Oxygen Consumption
Vascular Resistance
Lactic Acid
Necrosis
Glucose

Keywords

  • hyperthermia
  • isolation-perfusion
  • liver

ASJC Scopus subject areas

  • Surgery

Cite this

Zavagno, G., Vespa, D., Moschin, N., Belluco, C., Cecchetto, A., Bertocco, E., ... Lise, M. (1989). Effect of hyperthermia on isolated perfused rat liver. European Surgical Research, 21(5), 243-250.

Effect of hyperthermia on isolated perfused rat liver. / Zavagno, G.; Vespa, D.; Moschin, N.; Belluco, C.; Cecchetto, A.; Bertocco, E.; Raimondo, A.; Lise, M.

In: European Surgical Research, Vol. 21, No. 5, 1989, p. 243-250.

Research output: Contribution to journalArticle

Zavagno, G, Vespa, D, Moschin, N, Belluco, C, Cecchetto, A, Bertocco, E, Raimondo, A & Lise, M 1989, 'Effect of hyperthermia on isolated perfused rat liver', European Surgical Research, vol. 21, no. 5, pp. 243-250.
Zavagno G, Vespa D, Moschin N, Belluco C, Cecchetto A, Bertocco E et al. Effect of hyperthermia on isolated perfused rat liver. European Surgical Research. 1989;21(5):243-250.
Zavagno, G. ; Vespa, D. ; Moschin, N. ; Belluco, C. ; Cecchetto, A. ; Bertocco, E. ; Raimondo, A. ; Lise, M. / Effect of hyperthermia on isolated perfused rat liver. In: European Surgical Research. 1989 ; Vol. 21, No. 5. pp. 243-250.
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