Effect of hyperthermia on isolated perfused rat liver

G. Zavagno, D. Vespa, N. Moschin, C. Belluco, A. Cecchetto, E. Bertocco, A. Raimondo, M. Lise

Research output: Contribution to journalArticlepeer-review

Abstract

Four groups of isolated rat liver underwent perfusion at 37°C for 1 h, at 37, 40, 43 or 45°C, respectively, during the 2nd hour and then at 37°C again for the 3rd hour. Vascular resistance slightly decreased during hyperthermia and then significantly increased after restarting normothermic perfusion. At 40°C bile production, oxygen consumption, glucose and lactate release did not significantly differ from those found in the 37°C group. At 43 and 45°C all these parameters were significantly impaired when compared to the 37 and 40°C groups and did not recover after normothermic perfusion was restored. GOT and GPT release increased in proportion to the temperature. Microscopic examination revealed normal histologic features in 37 and 40°C specimens while alterations such as vacuolization and focal necrosis were found in the 43 and 45°C groups. These data indicate that the highest temperature that is well tolerated by isolated rat livers for 1 h is located between 40 and 43°C.

Original languageEnglish
Pages (from-to)243-250
Number of pages8
JournalEuropean Surgical Research
Volume21
Issue number5
Publication statusPublished - 1989

Keywords

  • hyperthermia
  • isolation-perfusion
  • liver

ASJC Scopus subject areas

  • Surgery

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