Effect of in vitro cadmium exposure on natural killer (NK) cells of catfish, ictalurus melas

A. Viola, G. Pregnolato, V. Albergoni

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of in vitro cadmium exposure on catfish natural killer (NK) cells was investigated. Purification of NK cells by nylon-wool columns and density gradient centrifugation indicated that they were a population of nylon-wool adherent cells. Subsequently NK cells were separated only by density gradient centrifugation, where these cells band at the 43/45·5% Percoll interface. In some cytotoxicity assays, catfish NK cells were exposed to 5, 10, 30 and 50 μM cadmium (CdCl2); 10 μl of Cd solutions were added to NK cells simultaneously with the target cells. In vitro, 5 μM Cd inhibited the ability of catfish NK cells to kill the human erythroleukemic cell line K-562. In the presence of 10 μM Cd, the antibody-independent cytotoxic activity was totally suppressed.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalFish and Shellfish Immunology
Volume6
Issue number3
DOIs
Publication statusPublished - Apr 1996

Keywords

  • Cadmium
  • Catfish
  • Natural killer cells

ASJC Scopus subject areas

  • Aquatic Science
  • Immunology

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