Effect of in vivo treatment with RH GM-CSF on in vitro growth of haematopoietic progenitors in patients with myelodysplastic syndromes

G. Visani, P. Tosi, C. Finelli, B. Gamberi, G. Zauli, A. Cenacchi, M. Fogli, R. Colombini, S. Tura

Research output: Contribution to journalArticlepeer-review

Abstract

Background. Recombinant (r) human (h) granulocyte/macrophage colony stimulating factor (rh GM-CSF) has been shown to increase the number of peripheral blood (PB) neutrophils, eosinophils and monocytes in myelodysplastic syndromes (MDS). The aims of this study were: 1) to evaluate the effect of rh GM-CSF therapy on the in vitro growth of granulocyte-erythroid-macrophage-megakaryocyte colonies (CFU-GEMM), erythroid colonies (BFU-E), and granulocyte-macrophage colonies (CFU-GM) in patients with MDS; 2) to assess in these patients, while they are being treated in vivo with rh GM-CSF, the possible additive effect of rh IL-3 and rh G-CSF on the in vitro growth of haematopoietic progenitors. Methods. The in vitro growth of CFU-GEMM, BFU-E and CFU-GM was studied in 10 myelodysplastic (MDS) patients, before and after in vivo administration of rh GM-CSF. Results. After rh GM-CSF administration, the number of CFU-GM increased in all standard risk MDS patients. In 2 out of 5 cases, this effect was more pronounced and persisted up to 30 days after the end of rh GM-CSF treatment. On the other hand, the number of CFU-GEMM and BFU-E was substantially unchanged. When rh GM-CSF, rh G-CSF and rh IL-3 were added in vitro alone or in combination as the source of colony stimulating activity, no significant increase of the CFU-GM colony number was noticed. Conclusions. Rh GM-CSF therapy appears useful for increasing the number of peripheral blood granulocytes and of marrow CFU-GM in standard-risk MDS patients. High-risk MDS patients are far less responsive to rh GM-CSF treatment, probably reflecting a more aggressive and/or advanced disease.

Original languageEnglish
Pages (from-to)142-145
Number of pages4
JournalHaematologica
Volume77
Issue number2
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Hematology

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