Effect of Inositol Hexakisphosphate on the Epr properties of the Nitric Oxide Derivative of Ferrous Dromedary (Camelus Dromedarius) Hemoglobin. Evidence for Two Polyanion Binding Sites

Alessandro Desideri, Paolo Ascenzi, Emilia Chiancone, Gino Amiconi

Research output: Contribution to journalArticle

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Abstract

The effect of inositol hexakisphosphate on the EPR properties of the nitric oxide derivative of ferrous dromedary (Camelus dromedarius) hemoglobin has been investigated at 110 K. In the absence of inositol hexakisphosphate, the nitrosyl derivative of dromedary hemoglobin shows an EPR spectrum with a rhombic shape and a weak hyperfine splitting in the gz region, a feature that is generally taken as characteristic of the high-affinity state of tetrameric hemoproteins. On addition of 1 mole of inositol hexakisphosphate/tetramer, three new hyperfine lines (Az =1.7 mT), centered at gz = 2.01, appear; this type of spectrum is indicative of the low-affinity state of hemoglobins. A further addition of inositol hexakisphosphate, corresponding to a 20-fold molar excess, completely reverses the polyphosphate-dependent transition, giving an EPR spectrum that is exactly superimposable to that observed in the absence of the allosteric effector, i.e., is typical of the high-affinity state of the macromolecule. Both in the absence and presence of inositol hexakisphosphate, the EPR spectra are virtually independent of pH in the range explored (from 5.5 to 7.5). These results, taken together with the behavior of the nitric oxide derivative of human hemoglobin, provide further evidence for the existance in dromedary hemoglobin of two polyanion binding sites that affect in an opposite way the conformational equilibrium of the macromolecule.

Original languageEnglish
Pages (from-to)131-135
Number of pages5
JournalJournal of Inorganic Biochemistry
Volume29
Issue number2
DOIs
Publication statusPublished - 1987

Fingerprint

Camelus
Phytic Acid
Nitric Oxide
Hemoglobins
Binding Sites
Paramagnetic resonance
Derivatives
Macromolecules
Polyphosphates
polyanions

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

Effect of Inositol Hexakisphosphate on the Epr properties of the Nitric Oxide Derivative of Ferrous Dromedary (Camelus Dromedarius) Hemoglobin. Evidence for Two Polyanion Binding Sites. / Desideri, Alessandro; Ascenzi, Paolo; Chiancone, Emilia; Amiconi, Gino.

In: Journal of Inorganic Biochemistry, Vol. 29, No. 2, 1987, p. 131-135.

Research output: Contribution to journalArticle

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